Agrobacterium-mediated transformation of Mycosphaerella fijiensis, the devastating Black Sigatoka pathogen of bananas

Mycosphaerella fijiensis, M. musicola en M. eumusae veroorzaken de Sigatoka-ziekte in banaan. Op dit moment is de toepassing van fungiciden de enige optie om deze ziekte te bestrijden. Het PRPB (Pesticide Reduction Program for Banana) investeert in de ontwikkeling van technieken voor de genotype- en fenotypebepaling van M. fijiensis. Hierbij wordt gebruikt gemaakt van ATMT (Agrobacterium tumefaciens-mediated transformation)

The merger history of massive spheroids since z~1 is size independent

Using a compilation of 379 massive (stellar mass M > 10^{11} M_Sun) spheroid-like galaxies from the near-infrared Palomar/DEEP-2 survey, we have probed, up to z~1, whether the presence of companions depends on the size of the host galaxies. We have explored the presence of companions with mass ratios down to 1:10 and 1:100, with respect to the central massive galaxy, and within a projected distance of 30, 50 and 100 kpc of these objects. We find evidence for these companions being equally distributed around both compact and extended massive spheroids. This finding suggests that, at least since z~1, the merger activity in these objects is rather homogeneous across the whole population and its merger history is not affected for the size of the host galaxy. Our result could indicate that both compact and extended massive spheroid-like galaxies are growing in size at the same rate.Comment: 9 pages, 6 figures, accepted for publication in MNRA

Conflictos sociales y mediación universitaria en el Espacio Europeo de Educación Superior

Este artículo expone los principales datos y conclusiones obtenidas en el Workshop realizado en el año 2016 entre la Universidad de Málaga y Leuven (Bélgica). La líneas de investigación se centra en los conflictos sociales que se producen en el ámbito universitario.El presente trabajo, trata de analizar los conflictos que se producen en el ámbito universitario relacionados con la implementación del Espacio Europeo de Educación Superior y la nueva metodología docente derivada del mismo, que provocan disyuntivas sociales específicas. Estos conflictos, se hacen plausibles en la conciliación, el aprendizaje y el desarrollo del proceso educativo. Por un lado, con la asistencia obligatoria y la dedicación de tiempo que requiere al estudiante en la Universidad y de trabajo en casa. Por otro, por la situación devenida de los recortes en becas universitarias y la necesidad que impone esta reforma educativa de acreditación del B1 como lengua extranjera para la obtención del Título de Graduado. Estas circunstancias, construyen realidades relativas a los significados que el ámbito universitario tiene para las personas, e inciden en las oportunidades del alumnado en condiciones de igualdad en función de su situación social y personal. En el estudio, se analizan las causas y se definen los tipos de conflictos, así como su relación con la citada reforma

Respuesta agronómica de diferentes cultivares tintos de vid en zona templada

Se presenta la respuesta varietal de 6 cultivares tintos de vid, con riego deficitario controlado, en zona cálida: Barbera, Graciano, Mencía, Pinot noir, Petit verdot y Tempranillo. Teniendo en cuenta que en zona cálida hay que escoger cultivares de ciclo largo, recolección tardía, capaces de madurar manteniendo alta la acidez real y total, se concluye que los más idóneos para su cultivo en zona cálida son Barbera, por su alta acidez y Petit verdot y Graciano por su larga maduración y acidez equilibrada. Tempranillo, si bien en este trabajo presenta una respuesta adecuada, para equivalente maduración sacarimétrica a las anteriores posee menor acidez e IPT. Mencía madura mejor en situaciones frescas que en zona cálid

Developing tools for Mycosphaerella fijiensis studies.

Mycosphaerella fijiensis is the causal agent of the black leaf streak disease, or Black Sigatoka, the most devasting fungal disease of banana around the world.Edição do Fast Firward 40 jaar Gewasbescherming, 2009, Wageningen

Luminous Satellites of Early-Type Galaxies I: Spatial Distribution

We study the spatial distribution of faint satellites of intermediate redshift (0.1<z<0.8), early-type galaxies, selected from the GOODS fields. We combine high resolution HST images and state-of-the-art host subtraction techniques to detect satellites of unprecedented faintness and proximity to intermediate redshift host galaxies (up to 5.5 magnitudes fainter and as close as 0."5/2.5 kpc to the host centers). We model the spatial distribution of objects near the hosts as a combination of an isotropic, homogenous background/foreground population and a satellite population with a power law radial profile and an elliptical angular distribution. We detect a significant population of satellites, Ns =1.7 (+0.9,-0.8) that is comparable to the number of Milky Way satellites with similar host-satellite contrast.The average projected radial profile of the satellite distribution is isothermal, gamma_p= -1.0(+0.3,-0.4), which is consistent with the observed central mass density profile of massive early-type galaxies. Furthermore, the satellite distribution is highly anisotropic (isotropy is ruled out at a >99.99% confidence level). Defining phi to be the offset between the major axis of the satellite spatial distribution and the major axis of the host light profile, we find a maximum posterior probability of phi = 0 and |phi| less than 42 degrees at the 68% confidence level. The alignment of the satellite distribution with the light of the host is consistent with simulations, assuming that light traces mass for the host galaxy as observed for lens galaxies. The anisotropy of the satellite population enhances its ability to produce the flux ratio anomalies observed in gravitationally lensed quasars.Comment: 21 pages, 16 figures, Accepted for publication in Ap

Thorough Characterization of ETHQB3.5, a QTL Involved in Melon Fruit Climacteric Behavior and Aroma Volatile Composition

The effect of the QTL involved in climacteric ripening ETHQB3.5 on the fruit VOC composition was studied using a set of Near-Isogenic Lines (NILs) containing overlapping introgressions from the Korean accession PI 16375 on the chromosome 3 in the climacteric ‘Piel de Sapo’ (PS) genetic background. ETHQB3.5 was mapped in an interval of 1.24 Mb that contained a NAC transcription factor. NIL fruits also showed differences in VOC composition belonging to acetate esters, non-acetate esters, and sulfur-derived families. Cosegregation of VOC composition (23 out of 48 total QTLs were mapped) and climacteric ripening was observed, suggesting a pleiotropic effect of ETHQB3.5. On the other hand, other VOCs (mainly alkanes, aldehydes, and ketones) showed a pattern of variation independent of ETHQB3.5 effects, indicating the presence of other genes controlling non-climacteric ripening VOCs. Network correlation analysis and hierarchical clustering found groups of highly correlated compounds and confirmed the involvement of the climacteric differences in compound classes and VOC differences. The modification of melon VOCs may be achieved with or without interfering with its physiological behavior, but it is likely that high relative concentrations of some type of ethylene-dependent esters could be achieved in climacteric cultivars.Publishe

Biological activity of 6,7-Dehydroxyroyleanone and derivatives obtained from Plectranthus aliciae (Codd) A.J.Paton

The Plectranthus genus (Lamiaceae) is known to be rich in abietane diterpenes. The bioactive 6,7-dehydroxyroyleanone (DHR, 1) was previously isolated from Plectranthus madagascariensis var. madagascariensis and var. aliciae. This study aimed to explore the occurrence of DHR, 1, in P. aliciae and the potential bioactivities of new semisynthetic derivatives from DHR, 1. Several extraction methods were evaluated, and the hydrodistillation, using a Clevenger apparatus, afforded the highest yield (77.8 mg/g of 1 in the essential oil). Three new acyl derivatives (2-4) were successfully prepared from 1 (yields of 86-95%). Compounds 1-4 showed antioxidant activity, antibacterial effects, potent cytotoxic activity against several cell lines, and enhanced anti-inflammatory activity that surpassed dexamethasone (positive control). These findings encourage further exploration of derivatives 2-4 for potential mechanisms of antitumoral, antioxidant, and anti-inflammatory capabilities, studying both safety and efficacy.This work was supported by the funding received by Fundação paraaCien̂ciaeTecnologia(FCT,Portugal)(projects DOI 10.54499/UIDP/04567/2020 and DOI 10.54499/UIDB/04567/2020 attributed to CBIOS, CIMO (UIDB/00690/2020 and UIDP/00690/2020), CESAM (UIDP/50017/2020, UIDB/50017/2020, LA/P/0094/2020), and SusTEC (LA/P/0007/2020) and a Ph.D. grant for F.M. (SFRH/BD/ 146614/2019). National funding was provided by FCT, P.I., through the institutional scientific employment program contracts (L.B.). E.M.D.-M. gratefully acknowledges being the recipient of a FPU-UAH 2019 contract and for the grant “Ayudas a la Movilidad del Personal Investigador en Formación 2022” from the University of Alcalá de Henares (UAH). The authors thank the laboratory CIMO-ESTIG for permission to use the balance ABT-100 ESM Kern (Balingen, Germany) and Escola Superior Agrária laboratory for the use of the mill IKA-WERKE M20.info:eu-repo/semantics/publishedVersio

Fossil groups origins II. Unveiling the formation of the brightest group galaxies through their scaling relations

(Abridged) Fossil systems are galaxy associations dominated by a relatively isolated, bright elliptical galaxy, surrounded by a group of smaller galaxies lacking L* objects. We analyzed the near-infrared photometric and structural properties of a sample of 20 BGGs present in FGs in order to better understand their formation mechanisms. Their surface-brightness distribution was fitted to a Sersic profile using the GASP2D algorithm. Then, the standard scaling relations were derived for the first time for these galaxies and compared with those of normal ellipticals and brightest cluster galaxies in non-fossil systems. The BGGs presented in this study represent a subset of the most massive galaxies in the Universe. We found that their ellipticity profiles are continuously increasing with the galactocentric radius. Our fossil BCGs follow closely the fundamental plane described by normal ellipticals. However, they depart from both the log \sigma_0 vs. log L_{K_{s}} and log r_{\rm e} vs. log L_{K_{s}} relations described by intermediate mass ellipticals. This occurs in the sense that our BGGs have larger effective radii and smaller velocity dispersions than those predicted by these relations. We also found that more elliptical galaxies systematically deviate from the previous relations while more rounder object do not. No similar correlation was found with the Sersic index. The derived scaling relations can be interpreted in terms of the formation scenario of the BGGs. Because our BGGs follow the fundamental plane tilt but they have larger effective radii than expected for intermediate mass ellipticals, we suggest that they only went through dissipational mergers in a early stage of their evolution and then assembled the bulk of their mass through subsequent dry mergers, contrary to previous claims that BGGs in FGs were formed mainly by the merging of gas-rich galaxies.Comment: 16 pages, 10 figures, accepted for publication at A&

Animal and Cellular Models of Alzheimer’s Disease: Progress, Promise, and Future Approaches

Alzheimer’s disease (AD) is an incurable neurodegenerative disease affecting over 45 million people worldwide. Transgenic mouse models have made remarkable contributions toward clarifying the pathophysiological mechanisms behind the clinical manifestations of AD. However, the limited ability of these in vivo models to accurately replicate the biology of the human disease have precluded the translation of promising preclinical therapies to the clinic. In this review, we highlight several major pathogenic mechanisms of AD that were discovered using transgenic mouse models. Moreover, we discuss the shortcomings of current animal models and the need to develop reliable models for the sporadic form of the disease, which accounts for the majority of AD cases, as well as human cellular models to improve success in translating results into human treatments.Peer reviewe