345 research outputs found

    Detailed study of perforated beams with closely spaced novel web opening.

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    This paper presents a detailed study of the behaviour of perforated steel beams with closely spaced web openings. Seven specimens including two typical cellular beams (i.e. circular web openings) and five perforated beams with novel web opening shapes were tested previously by the authors, to investigate the failure mode and load strength of the web-post between two adjacent web openings. These new novel web opening shapes improve the structural performance of the perforated beams with respect to web-post buckling failure. In addition, the manufacturing procedure of these novel web openings is improved and leads to sustainable design. The effects of web opening spacing/web opening depth of web-posts as well as the web opening depth/web thickness were studied to investigate the stability (slenderness) of the web-post subjected to vertical shear load. In comparison with the conventional cellular beams, significant advantages were obtained

    Re-programming mouse liver-resident invariant natural killer T cells for suppressing hepatic and diabetogenic autoimmunity

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    Invariant NKT (iNKT) cells comprise a heterogeneous group of non-circulating, tissue-resident T lymphocytes that recognize glycolipids, including alpha-galactosylceramide (?GalCer), in the context of CD1d, but whether peripheral iNKT cell subsets are terminally differentiated remains unclear. Here we show that mouse and human liver-resident ?GalCer/CD1d-binding iNKTs largely correspond to a novel Zbtb16+Tbx21+Gata3+MaflowRorc- subset that exhibits profound transcriptional, phenotypic and functional plasticity. Repetitive in vivo encounters of these liver iNKT (LiNKT) cells with intravenously delivered ?GalCer/CD1d-coated nanoparticles (NP) trigger their differentiation into immunoregulatory, IL-10+IL-21-producing Zbtb16highMafhighTbx21+Gata3+Rorc- cells, termed LiNKTR1, expressing a T regulatory type 1 (TR1)-like transcriptional signature. This response is LiNKT-specific, since neither lung nor splenic tissue-resident iNKT cells from ?GalCer/CD1d-NP-treated mice produce IL-10 or IL-21. Additionally, these LiNKTR1 cells suppress autoantigen presentation, and recognize CD1d expressed on conventional B cells to induce IL-10+IL-35-producing regulatory B (Breg) cells, leading to the suppression of liver and pancreas autoimmunity. Our results thus suggest that LiNKT cells are plastic for further functional diversification, with such plasticity potentially targetable for suppressing tissue-specific inflammatory phenomena.© 2022. The Author(s)
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