65 research outputs found

    Detection of bovine leucocyte adhesion deficiency [BLAD] carriers using a new PCR test

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    In this report we demonstrate a simple, effective and reliable diagnostic test of BLAD carrier detection based on specific PCR amplification of a 367 bp CD18 gene fragment and RFLP analysis using Taq I restriction enzyme. In a non-random population of 220 animals we found 48 BLAD carriers. Within the amplified PCR fragment an unknown intron sequence of 159 bp was identified

    The PRNP ins/del

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