PDlim2 Selectively Interacts with the PDZ Binding Motif of Highly Pathogenic Avian H5N1 Influenza A Virus NS1

The multi-functional NS1 protein of influenza A virus is a viral virulence determining factor. The last four residues at the C-terminus of NS1 constitute a type I PDZ domain binding motif (PBM). Avian influenza viruses currently in circulation carry an NS1 PBM with consensus sequence ESEV, whereas human influenza viruses bear an NS1 PBM with consensus sequence RSKV or RSEV. The PBM sequence of the influenza A virus NS1 is reported to contribute to high viral pathogenicity in animal studies. Here, we report the identification of PDlim2 as a novel binding target of the highly pathogenic avian influenza virus H5N1 strain with an NS1 PBM of ESEV (A/Chicken/Henan/12/2004/H5N1, HN12-NS1) by yeast two-hybrid screening. The interaction was confirmed by in vitro GST pull-down assays, as well as by in vivo mammalian two-hybrid assays and bimolecular fluorescence complementation assays. The binding was also confirmed to be mediated by the interaction of the PDlim2 PDZ domain with the NS1 PBM motif. Interestingly, our assays showed that PDlim2 bound specifically with HN12-NS1, but exhibited no binding to NS1 from a human influenza H1N1 virus bearing an RSEV PBM (A/Puerto Rico/8/34/H1N1, PR8-NS1). A crystal structure of the PDlim2 PDZ domain fused with the C-terminal hexapeptide from HN12-NS1, together with GST pull-down assays on PDlim2 mutants, reveals that residues Arg16 and Lys31 of PDlim2 are critical for the binding between PDlim2 and HN12-NS1. The identification of a selective binding target of HN12-NS1 (ESEV), but not PR8-NS1 (RSEV), enables us to propose a structural mechanism for the interaction between NS1 PBM and PDlim2 or other PDZ-containing proteins

An Adaptive Pose Fusion Method for Indoor Map Construction

The vision-based robot pose estimation and mapping system has the disadvantage of low pose estimation accuracy and poor local detail mapping effects, while the modeling environment has poor features, high dynamics, weak light, and multiple shadows, among others. To address these issues, we propose an adaptive pose fusion (APF) method to fuse the robot’s pose and use the optimized pose to construct an indoor map. Firstly, the proposed method calculates the robot’s pose by the camera and inertial measurement unit (IMU), respectively. Then, the pose fusion method is adaptively selected according to the motion state of the robot. When the robot is in a static state, the proposed method directly uses the extended Kalman filter (EKF) method to fuse camera and IMU data. When the robot is in a motive state, the weighted coefficient is determined according to the matching success rate of the feature points, and the weighted pose fusion (WPF) method is used to fuse camera and IMU data. According to the different states, a series of new poses of the robot are obtained. Secondly, the fusion optimized pose is used to correct the distance and azimuth angle of the laser points obtained by LiDAR, and a Gauss–Newton iterative matching process is used to match the corresponding laser points to construct an indoor map. Finally, a pose fusion experiment is designed, and the EuRoc data and the measured data are used to verify the effectiveness of this method. The experimental results confirm that this method provides higher pose estimation accuracy compared with the robust visual inertial odometry (ROVIO) and visual-inertial ORB-SLAM (VI ORB-SLAM) algorithms. Compared with the Cartographer algorithm, this method provides higher two-dimensional map modeling accuracy and modeling performance

Localization and analysis of synthetic gene clusters of <i>Bacillus velenzensis</i> S3-1 in surfactin,iturin and fengycin

In this study,the GenBank database was used to search for Bacillus which could produce surfactin,iturin or fengycin and its synthetic genes were annotated in the genome.The location of the synthetic gene clusters of these three lipopeptide antibiotics were determined by BLAST alignment.Then antiSMASH was used to determine the final location of the synthetic gene clusters.Further,we predicted and analyzed the structure and related synthetase domains of the surfactin,iturin and fengycin

Treatment of pediatric H-type rectovestibular fistula with tension-free repair of rectal seromuscular layer

Objective: This study aimed to assess the outcomes of a 7-year experience with tension-free repair of the rectal seromuscular layer for H-type rectovestibular fistula in female children with a normal anus. Methods: Between May 2016 and January 2023, 86 patients with H-type rectovestibular fistula underwent a standardized surgical procedure conducted by the same surgical team. The procedure involved: (1) Identifying and locating the vestibular orifice. (2) Dissecting the fistula. (3) Dissecting the anterior rectal wall. (4) Repairing the fistula. (5) Closing the internal opening of the fistula. During the dissection of the rectovaginal septum, care was taken to avoid any harm to the posterior wall of the vagina while exposing the anterior rectal wall by 10–25 mm. Results: Follow-up, conducted via telephone or outpatient visits, ranged from 3 months to 6 years and 11 months (median, 3.5 years). In 82 cases (95.35% of 86), primary healing was achieved, resulting in satisfactory perineal appearance, smooth stool passage, and regular defecation. In 4 cases (4.65% of 86), fistula recurrence occurred within 5 to 10 days post-surgery. One case healed within 3 weeks with 3% boric acid sitz baths. The other 2 cases underwent debridement 7 days after the initial operation, leading to successful recovery. The final case experienced a recurrence 1 year after surgery following resection and repair of the perineal fistula, and no further surgical intervention was pursued. Conclusion: Tension-free repair of the rectal seromuscular layer represents a straightforward, safe, and effective surgical approach for managing H-type rectovestibular fistula with a normal anus in female children

BRS1 Function in Facilitating Lateral Root Emergence in Arabidopsis

The BRS1 (BRI1 Suppressor 1) gene encodes a serine carboxypeptidase that plays a critical role in the brassinosteroid signaling pathway. However, its specific biological function remains unclear. In this study, the developmental role of BRS1 was investigated in Arabidopsis thaliana. We found that overexpressing BRS1 resulted in significantly more lateral roots in different Arabidopsis ecotypes (WS2 and Col-0) and in brassinosteroid mutants (bri1-5 and det2-28). Further research showed that BRS1 facilitates the process whereby lateral root primordia break through the endodermis, cortex, and epidermis. Consistent with this, BRS1 was found to be highly expressed in the root endodermis and accumulated in the extracellular space around the dome of the lateral root primordia. Taken together, these results highlight the role of BRS1 in the process of lateral root emergence and provide new insight into the role of serine carboxypeptidases in plant root development

Serum Metabolomics Analysis of Asthma in Different Inflammatory Phenotypes: A Cross-Sectional Study in Northeast China

Background and Objective. Asthma as a chronic heterogeneous disease seriously affects the quality of life. Incorrect identification for its clinical phenotypes lead to a huge waste of medical resources. Metabolomic technique as a novel approach to explore the pathogenesis of diseases have not been used to study asthma based on their clear defined inflammatory phenotypes. This study is aimed to distinguish the divergent metabolic profile in different asthma phenotypes and clarify the pathogenesis of them. Methods. Participants including eosinophilic asthmatics (EA, n=13), noneosinophilic asthmatics (NEA, n=16), and healthy controls (HC, n=15) were enrolled. A global profile of untargeted serum metabolomics was identified with Ultra Performance Liquid Chromatography–Mass Spectrometry technique. Results. Multivariate analysis was performed and showed a clear distinction between EA, NEA, and HC. A total of 18 different metabolites were recognized between the three groups based on OPLS-DA model and involved in 10 perturbed metabolic pathways. Glycerophospholipid metabolism, retinol metabolism, and sphingolipid metabolism were identified as the most significant changed three pathways (impact > 0.1 and -log(P) > 4) between the phenotypes. Conclusions. We showed that the different inflammatory phenotypes of asthma involve the immune regulation, energy, and nutrients metabolism. The clarified metabolic profile contributes to understanding the pathophysiology of asthma phenotypes and optimizing the therapeutic strategy against asthma heterogeneity

Screening of chlorpyrifos degrading bacteria CD7 and its combined application with PGPR JD37

We screened a chlorpyrifos degrading bacteria,Burkholderiasp. CD7.Joint with plant growth-promoting rhizobacteria(PGPR) JD37 to produce a compositesoil amendment,which could restorethe pesticides polluted soil and promote plant growth.Results showed that CD7 and JD37 (at the volume ratio of 1:1) can promote the growth of plants,and within 25 days degrade about 66.43% chlorpyrifos in the soil.Further research found that under the same conditions of carrier dosage,vermicompost can adsorbed more bacteria than talcum powder;after a month preservation at room temperature,the number of living bacterium still maintained about 4.81&#215;107 CFU/g.Carrier and soil,at the mass ratio of 1:1,could optimally promote plant growth,improve soil enzyme activities and increase the number of microorganisms in soil

Coixendide efficacy in combination with temozolomide in glioblastoma and transcriptome analysis of the mechanism

Abstract The purpose of this study was to explore the role of coixendide (Coix) combine with temozolomide (TMZ) in the treatment of Glioblastoma (GBM) and explore its possible mechanism. CCK-8 was used to determine the inhibitory rate of Coix group, TMZ group and drug combination group on GBM cells, and the combination index (CI) was calculated to determine whether they had synergistic effect. Then RNA was extracted from each group, transcriptome sequencing was performed, and differentially expressed genes (DEGs) were identified. The possible mechanism was analyzed by GO enrichment analysis and KEGG enrichment analysis. The CI of Coix and TMZ indicating a synergistic effect when TMZ concentration is 0.1 mg/ml and Coix concentration is 2 mg/ml. Transcriptome sequencing analysis showed that interferon (IFN) related genes were down-regulated by Coix and up-regulated by TMZ and combined drugs, however, the up-regulation induced by combined drugs was less than that of TMZ. Besides IFN related genes, cholesterol metabolism pathway were also been regulated. Coix and TMZ have synergistic effects in the treatment of GBM at certain doses. RNA-Seq results suggested that the abnormal on genetic materials caused by DNA damage induced by TMZ treatment can be sensed by IFN related genes and activates antiviral IFN signaling, causing the activation of repairing mechanism and drug resistance. Coix inhibits IFN related genes, thereby inhibits drug resistance of TMZ. In addition, the activation of ferroptosis and the regulation of DEGs in cholesterol metabolism pathway were also contributed to the synergistic effects of Coix and TMZ