Fire dynamics and driving mechanisms on the Eastern Coast of China since the Late Pleistocene: evidence from charcoal records on Shengshan Island

Fires play a significant role in ecosystems, exerting a profound influence on climate, vegetation, and geochemical cycles, while being reciprocally affected by these factors. The reconstruction of past fire events serves as a valuable window into understanding environmental changes over time. To investigate the history of ancient fires on the Eastern Coast of China, we conducted the first charcoal analysis on a loess profile of Shengshan Island (East China Sea). Along with other biological and geochemical proxies, we successfully reconstructed the ancient fire dynamics and elucidated their driving mechanisms in this region since the Late Pleistocene. Our initial findings revealed a peak in charcoal concentration during the 60-50 ka period, but after calibrating for sedimentation rate, the concentration significantly decreased. Fire activities remained weak during 50-30 ka, likely due to the scarcity of combustible materials. Between 30-12 ka, fires were frequent in the early period, while gradually diminishing during the later stage. Dry climate and dense vegetation likely attributed to frequent fires in early period, while some extreme events (e.g., sudden change in temperature) may have decreased the fire frequency in later period. The Holocene (began ~12 ka) evidenced the most frequent fire events as a high charcoal concentration was recorded, likely caused by human activities. After comparing our findings with other paleoecological records from surrounding areas, we confirmed the accuracy of our reconstruction of ancient fires. This reconstruction captures not only local shifts but also broader regional changes. Overall, our study highlights the importance of calibrating sedimentation rate in charcoal profiles, while also contributing to an enhanced understanding of environmental changes along the Eastern Coast of China since the Late Pleistocene

Seismic activities before and after the impoundment of the Xiangjiaba and Xiluodu reservoirs in the lower Jinsha River

The lower Jinsha River basin is located at the junction of Sichuan and Yunnan provinces in Southwest China, a region with intense tectonic movements and frequent moderate to strong seismic activities. Cascade hydropower stations have been constructed along the lower Jinsha River since 2012. However, research on the effect of the impoundment of large-scale cascade reservoirs in a river basin on local seismic activities is currently lacking. Accurately identifying earthquake locations is essential for studying reservoir-induced earthquakes. Analyzing the spatiotemporal migration process of seismic activities based on complete and precise earthquake relocation is fundamental for determining the fluid diffusion coefficient, constructing fault models for reservoir areas, identifying earthquake types, exploring earthquake mechanisms, and evaluating seismic hazards. The seismicity pattern in the Xiangjiaba and Xiluodu reservoir areas, where seismic activities had been weak for a long time, has changed with the successive impoundment of the two reservoirs, showing microseismic events and seismic clusters. We investigated the spatiotemporal characteristics of seismic activities in the Xiangjiaba and Xiluodu reservoir areas using the waveform cross-correlation-based double-difference relocation technique and the b-value analysis method. We discovered that seismic events after the impoundment of these two reservoirs exhibited different characteristics in different regions. The seismic activities at the Xiluodu dam quickly responded to the rising water level, with the seismic intensity decaying rapidly afterward. These events were concentrated in the limestone strata along both sides of the Jinsha River, with a shallow focal depth, generally within 5 km, and a high b-value of approximately 1.2. Such features are close to those of karst-type earthquakes. Microseismic activities frequent occur on the eastern bank of the Yongshan reservoir section downstream of the Xiluodu dam, with two parallel NW-trending earthquake strips visible after precise earthquake relocation. The MS5.2 earthquake near Wuji town on August 17, 2014, had prominent foreshocks and aftershocks distributed in a clear NW-trending 20-km-long strip, perpendicular to the riverbank. These seismic events had a low b-value of approximately 0.7. The orientation of the node plane revealed by the strike-slip focal mechanism of the mainshock is consistent with that of the strip formed by the foreshock-mainshock-aftershock sequence, indicating the existence of a NW-striking concealed fault. Seismic activities near the Yanjin-Mabian fault upstream of the Xiangjiaba reservoir area since 2013 were concentrated in a NW-trending strip, with several near EW-trending seismic clusters on its western side, and with the largest event having a magnitude of ML3.7. So far, the impoundment of the Xiangjiaba and Xiluodu reservoirs has not triggered seismic activities on the large Jinyang-Ebian and Yanjin-Mabian faults nearby

Improved Simulated-Daylight Photodynamic Therapy and Possible Mechanism of Ag-Modified TiO₂ on Melanoma

Simulated-daylight photodynamic therapy (SD-PDT) may be an efficacious strategy for treating melanoma because it can overcome the severe stinging pain, erythema, and edema experienced during conventional PDT. However, the poor daylight response of existing common photosensitizers leads to unsatisfactory anti-tumor therapeutic effects and limits the development of daylight PDT. Hence, in this study, we utilized Ag nanoparticles to adjust the daylight response of TiO2, acquire efficient photochemical activity, and then enhance the anti-tumor therapeutic effect of SD-PDT on melanoma. The synthesized Ag-doped TiO2 showed an optimal enhanced effect compared to Ag-core TiO2. Doping Ag into TiO2 produced a new shallow acceptor impurity level in the energy band structure, which expanded optical absorption in the range of 400–800 nm, and finally improved the photodamage effect of TiO2 under SD irradiation. Plasmonic near-field distributions were enhanced due to the high refractive index of TiO2 at the Ag-TiO2 interface, and then the amount of light captured by TiO2 was increased to induce the enhanced SD-PDT effect of Ag-core TiO2. Hence, Ag could effectively improve the photochemical activity and SD-PDT effect of TiO2 through the change in the energy band structure. Generally, Ag-doped TiO2 is a promising photosensitizer agent for treating melanoma via SD-PDT

Exogenous tumor necrosis factor-alpha could induce egress of Toxoplasma gondii from human foreskin fibroblast cells

Toxoplasma gondii is an intra-cellular protozoan parasite that can infect almost all nucleated cells, eliciting host immune responses against infection. Host tissue damage is mainly caused by cellular lysis when T. gondii egresses from infected cells. However, the effects of cytokines released by host immune cells on egression of T. gondii remain elusive. This study aimed to investigate the role of tumor necrosis factor-alpha (TNF-α) on the egress of T. gondii from infected human foreskin fibroblast (HFF) cells and to elucidate the underlying mechanisms that regulate TNF-α-induced egress. Using flow cytometry to count tachyzoites of T. gondii released into cell culture medium, we found that egress of T. gondii from infected HFF cells could be induced by 10 ng/mL TNF-α in a time-dependent manner. Pre-treatment of infected HFF cells with BAPTA-AM to chelate intra-parasitic calcium could greatly inhibit TNF-α-induced egress. Similar results were obtained when using cytochalasin D to block parasite motility before the TNF-α-induced egress assay. In addition, blocking host apoptosis by Z-VAD-FMK could decrease TNF-α induced egress, while blocking necroptosis by necrostatin-1 has little impact on TNF-α-induced egress. The egressed tachyzoites displayed a normal growth rate and lost no virulence. Our results suggest that host cytokines could influence the cellular lytic processes of T. gondii, providing new insights into the relationship between host TNF-α and T. gondii pathogenesis

Exogenous tumor necrosis factor-alpha could induce egress of

Toxoplasma gondii is an intra-cellular protozoan parasite that can infect almost all nucleated cells, eliciting host immune responses against infection. Host tissue damage is mainly caused by cellular lysis when T. gondii egresses from infected cells. However, the effects of cytokines released by host immune cells on egression of T. gondii remain elusive. This study aimed to investigate the role of tumor necrosis factor-alpha (TNF-α) on the egress of T. gondii from infected human foreskin fibroblast (HFF) cells and to elucidate the underlying mechanisms that regulate TNF-α-induced egress. Using flow cytometry to count tachyzoites of T. gondii released into cell culture medium, we found that egress of T. gondii from infected HFF cells could be induced by 10 ng/mL TNF-α in a time-dependent manner. Pre-treatment of infected HFF cells with BAPTA-AM to chelate intra-parasitic calcium could greatly inhibit TNF-α-induced egress. Similar results were obtained when using cytochalasin D to block parasite motility before the TNF-α-induced egress assay. In addition, blocking host apoptosis by Z-VAD-FMK could decrease TNF-α induced egress, while blocking necroptosis by necrostatin-1 has little impact on TNF-α-induced egress. The egressed tachyzoites displayed a normal growth rate and lost no virulence. Our results suggest that host cytokines could influence the cellular lytic processes of T. gondii, providing new insights into the relationship between host TNF-α and T. gondii pathogenesis

Influence of Parameters on Photodynamic Therapy of Au@TiO2–HMME Core-Shell Nanostructures

Photodynamic therapy (PDT) is a promising tumor therapy and has been proven to be an effective, safe and minimally invasive technique. Hematoporphyrin monomethyl ether (HMME) mediated PDT has been used in clinical treatment of port wine stain (PWS) due to its single component, high yield of singlet oxygen and short light-sensitive period. However, as an amphiphilic photosensitizer, HMME is easy to aggregate due to the presence of a hydrophobic group, which undesirably reduced its generation of singlet oxygen and bioavailability. In this study, we synthesized the stable conjugate of Au@TiO2 core-shell nanostructure with HMME, and the influence of different factors on PTD efficiency were studied. The results showed that the nanostructure had higher PTD efficiency for KB cells than that of HMME. The irradiation wavelength, gold nanoparticle shape and the shell thickness are all important factors for KB cell PDT