Early Second-Trimester Serum MiRNA Profiling Predicts Gestational Diabetes Mellitus

BACKGROUND: Gestational diabetes mellitus (GDM) is one type of diabetes that presents during pregnancy and significantly increases the risk of a number of adverse consequences for the fetus and mother. The microRNAs (miRNA) have recently been demonstrated to abundantly and stably exist in serum and to be potentially disease-specific. However, no reported study investigates the associations between serum miRNA and GDM. METHODOLOGY/PRINCIPAL FINDINGS: We systematically used the TaqMan Low Density Array followed by individual quantitative reverse transcription polymerase chain reaction assays to screen miRNAs in serum collected at 16-19 gestational weeks. The expression levels of three miRNAs (miR-132, miR-29a and miR-222) were significantly decreased in GDM women with respect to the controls in similar gestational weeks in our discovery evaluation and internal validation, and two miRNAs (miR-29a and miR-222) were also consistently validated in two-centric external validation sample sets. In addition, the knockdown of miR-29a could increase Insulin-induced gene 1 (Insig1) expression level and subsequently the level of Phosphoenolpyruvate Carboxy Kinase2 (PCK2) in HepG2 cell lines. CONCLUSIONS/SIGNIFICANCE: Serum miRNAs are differentially expressed between GDM women and controls and could be candidate biomarkers for predicting GDM. The utility of miR-29a, miR-222 and miR-132 as serum-based non-invasive biomarkers warrants further evaluation and optimization

Body Mass Index and Diabetes in Asia: A Cross-Sectional Pooled Analysis of 900,000 Individuals in the Asia Cohort Consortium

10.1371/journal.pone.0019930PLoS ONE66

Improvement to the Huff Curve for Design Storms and Urban Flooding Simulations in Guangzhou, China

The storm hyetograph is critical in drainage design since it determines the peak flooding volume in a catchment and the corresponding drainage capacity demand for a return period. This study firstly compares the common design storms such as the Chicago, Huff, and Triangular curves employed to represent the storm hyetographs in the metropolitan area of Guangzhou using minute-interval rainfall data during 2008–2012. These common design storms cannot satisfactorily represent the storm hyetographs in sub-tropic areas of Guangzhou. The normalized time of peak rainfall is at 33 ± 5% for all storms in the Tianhe and Panyu districts, and most storms (84%) are in the 1st and 2nd quartiles. The Huff curves are further improved by separately describing the rising and falling limbs instead of classifying all storms into four quartiles. The optimal time intervals are 1–5 min for deriving a practical urban design storm, especially for short-duration and intense storms in Guangzhou. Compared to the 71 observed storm hyetographs, the Improved Huff curves have smaller RMSE and higher NSE values (6.43, 0.66) than those of the original Huff (6.62, 0.63), Triangular (7.38, 0.55), and Chicago (7.57, 0.54) curves. The mean relative difference of peak flooding volume simulated with SWMM using the Improved Huff curve as the input is only 2%, −6%, and 8% of those simulated by observed rainfall at the three catchments, respectively. In contrast, those simulated by the original Huff (−12%, −43%, −16%), Triangular (−22%, −62%, −38%), and Chicago curves (−17%, −19%, −21%) are much smaller and greatly underestimate the peak flooding volume. The Improved Huff curve has great potential in storm water management such as flooding risk mapping and drainage facility design, after further validation

A New Urban Waterlogging Simulation Method Based on Multi-Factor Correlation

Waterlogging simulation is a key technology for solving urban waterlogging problems. The current waterlogging modeling process is relatively complex and requires high basic data, which is not conducive to rapid modeling and popularization. In this study, we evaluated the correlation between rainfall and waterlogging water using the following factors: terrain, evaporation, infiltration, pipe drainage capacity, and river flood water level. By quantifying the influence value of each factor on rainfall, we established a simplified model for fast calculation of waterlogging depth through input rainfall. Waterlogging data was collected from Guangzhou, China to set up the multi-factor correlation model, and verify the simulation results of the model. After the original rainfall is added/deducted, the added/loss value, the relationship between net rainfall, and maximum water depth is better than that between original rainfall and maximum water depth. Establishing a stable multi-factor correlation model for a waterlogging point requires at least three historical waterlogging event data for parameter calibration by sensitivity analysis. Comparing the simulation of four waterlogging points, the multi-factor correlation model (error = −13%) presented the least error in simulating the maximum water volume, followed by the Mike Urban model (error = −19%), and finally the SWMM model (error = 20%). Furthermore, the multi-factor correlation model and SWMM model required the least calculation time (less than 1 s), followed by the Mike Urban model (About half a minute). By analyzing the waterlogging data of Guangzhou, 42 waterlogging points with modeling conditions were screened out to further validate the multi-factor correlation model. Each waterlogging point was modeled based on the historical field, and the last rainstorm was used for model verification. The mean error of the comparison between the simulated maximum waterlogging and the measured maximum waterlogging was 3%, and the R2 value was 0.718. In summary, the multi-factor correlation model requires fewer basic data, has a simple modeling process and wide applicability, and makes it easy to realize the intelligent parameter adjustment, which is more suitable for the urgent requirements of current urban waterlogging prediction. The model results may prove accurate and provide scientific decision support for the prevention and control of urban waterlogging

Characteristics of Heavy Storms and the Scaling Relation with Air Temperature by Event Process-Based Analysis in South China

The negative scaling rate between precipitation extremes and the air temperature in tropic and subtropic regions is still a puzzling issue. This study investigates the scaling rate from two aspects, storm characteristics (types) and event process-based temperature variations. Heavy storms in South China are developed by different weather systems with unique meteorological characteristics each season, such as the warm-front storms (January), cold-front storms (April to mid-May), monsoon storms (late May to June), convective storms, and typhoon storms (July to September). This study analyzes the storm characteristics using the hourly rainfall data from 1990 to 2017; compares the storm hyetographs derived from the one-minute rainfall data during 2008–2017; and investigates the interactions between heavy storms and meteorological factors including air temperature, relative humidity, surface pressure, and wind speed at 42 weather stations in Guangzhou during 2015–2017. Most storms, except for typhoon and warm-front storms, had a short duration (3 h) and intense rates (~13 mm/h) in Guangzhou, South China. Convective storms were dominant (50%) in occurrence and had the strongest intensity (15.8 mm/h). Storms in urban areas had stronger interactions with meteorological factors and showed different hyetographs from suburban areas. Meteorological factors had larger variations with the storms that occurred in the day time than at night. The air temperature could rise 6 °C and drop 4 °C prior to and post-summer storms against the diurnal mean state. The 24-hour mean air temperature prior to the storms produced more reliable scaling rates than the naturally daily mean air temperature. The precipitation extremes showed a peak-like scaling relation with the 24-hour mean air temperature and had a break temperature of 28 °C. Below 28 °C, the relative humidity was 80%–100%, and it showed a positive scaling rate. Above 28 °C, the negative scaling relation was likely caused by a lack of moisture in the atmosphere, where the relative humidity decreased with the air temperature increase

Performance Evaluation of Real-Time Precise Point Positioning with Both BDS-3 and BDS-2 Observations

For time-critical precise applications, one popular technology is the real-time precise point positioning (PPP). In recent years, there has been a rapid development in the BeiDou Navigation Satellite System (BDS), and the constellation of global BDS (BDS-3) has been fully deployed. In addition to the regional BDS (BDS-2) constellation, the real-time stream CLK93 has started to support the BDS-3 constellation, indicating that the real-time PPP processing involving BDS-3 observations is feasible. In this study, the global positioning performance of real-time PPP with BDS-3/BDS-2 observations is initially evaluated using the datasets from 147 stations. In the east, north and upward directions, positioning accuracy of 1.8, 1.2 and 2.5 cm in the static mode, and of 6.7, 5.1 and 10.4 cm in the kinematic mode can be achieved for the BDS-3/BDS-2 real-time PPP, respectively, while the corresponding convergence time with a threshold of 10 cm is 32.9, 23.7 and 32.8 min, and 66.9, 42.9 and 69.1 min in the two modes in the three directions, respectively. To complete this, the availability of BDS-3/BDS-2 constellations, the quality of BDS-3/BDS-2 real-time precise satellite products, and the BDS-3/BDS-2 post-processed PPP solutions are also analyzed. For comparison, the results for the GPS are also presented