Media 1: Axial nano-displacement measurement with high resolution and wide range based on asymmetrical illumination

Originally published in Optics Express on 25 March 2013 (oe-21-6-7528

procedure.mp4

Procedure for virtual modulatio

Ketocoumarin-Based Photoinitiators for High-Sensitivity Two-Photon Lithography

Ketocoumarins are attractive and distinct photosensitizers due to their high molar extinction coefficients, high intersystem crossing coefficients, and high photochemical stability. As a classic commercial ketocoumarin-based two-photon initiator, 7-diethylamino-3-thenoylcoumarin (DETC) was widely used in two-photon lithography. However, the large fluorescence quantum yield and low two-photon absorption cross section value greatly limit its application in high-throughput nanofabrication. In this work, a series of DETC derivatives were developed by extending the length of the alkyl chain and integrating different donor and acceptor groups. These ketocoumarin-based initiators, namely, compounds 1–7, were designed, synthesized, and unambiguously characterized. Compared with DETC, these compounds exhibit higher molar extinction coefficient, lower fluorescence quantum yield, higher two-photon absorption cross section, and improved sensitivity in two-photon lithography. Among these molecules, compound 7 with expanded π-electron systems and structures with enhanced intramolecular charge transfer exhibits the best sensitivity in two-photon lithography. With compound 7-based photoresist, many kinds of complex three-dimensional patterns can be fabricated using two-photon lithography at a writing speed of up to 60 mm s–1. The high two-photon initiation sensitivity makes these compounds promising candidates for commercialization and provides a new design concept for the development of new initiators

Supplementary document for Untrained neural network enables fast structured illumination microscopy - 6877193.pdf

supplement 1. Supplementary material for USRNN, including training details and comparison with other algorithm

Supplementary document for Effects of optical aberrations on localization of MINFLUX super-resolution microscopy - 6151527.pdf

Supplement

Supplement 1: Saturated absorption competition microscopy

Supplement 1 Originally published in Optica on 20 June 2017 (optica-4-6-633

Photon-Weight-Reallocated Stimulated Emission Depletion Nanoscopy

A novel optimization concept called “photon weight reallocation” is proposed to evaluate the significance of individual photons in an image, filtering out useless photons while preserving effective ones. This process aims to minimize background noise and enhance the resolution of the image. By leveraging a honeycomb array detector and fluorescence lifetime detection, photon weight reallocation is implemented in stimulated emission depletion (STED) nanoscopy through the fusion of the spatial and temporal photon weights. This approach yields exceptional results in terms of background and noise suppression and resolution enhancement. The experimental results demonstrate significant improvements in both the signal-to-background ratio and peak signal-to-background ratio within dense sample areas of STED after applying the photon-weight-reallocation process, achieving maximum improvements by factors of 8.9 and 37.3, respectively. Besides, the axial and lateral resolutions of STED are improved by factors of 1.55 and 1.36, respectively, following this process

Visualization 2. Three dimensional high resolution mitochondria fission and fusion dynamics.mp4

Three-dimensional high resolution mitochondria fission and fusion dynamic

Visualization 1: Digital micromirror device-based laser-illumination Fourier ptychographic microscopy

The reconstruction of the sample information in spatial and Fourier domain implemented by running the self-developed software written in Matlab Originally published in Optics Express on 19 October 2015 (oe-23-21-26999