Optimization of Glycosaminoglycan Extraction on Patinopecten Yessoensis Waste

AbstractOn the basis of single factor experiment, the pH of enzymatic hydrolysis, enzymolysis temperature, enzymolysis time and solid-liquid ratio as independent variable, extraction rate as response value, extraction technology of glycosaminoglycan from Patinopecten yessoensis waste were optimized using response surface methodology. The order affecting glycosaminoglycan extraction rate was determined: the enzymatic pH > solid-liquid ratio > enzymatic time > enzymatic temperature. The optimal conditions of extraction were: the pH of enzymatic hydrolysis was 8.0, enzymolysis temperature was 40°C, enzymolysis time was 3.5h and solid-liquid ratio was 1:2. Click here and insert your abstract text

Combined transepithelial phototherapeutic keratectomy and corneal collagen cross-linking for corneal ectasia after small-incision lenticule extraction—preoperative and 3-year postoperative results: a case report

Abstract Background Corneal ectasia after small-incision lenticule extraction (SMILE) is uncommon. To our knowledge, this is the first report of 3-year results of combined phototherapeutic keratectomy (PTK) and corneal collagen cross-linking (CXL) for corneal ectasia after SMILE. Case presentation Herein, we describe a case of prominent corneal ectasia after SMILE treated with PTK combined with CXL 3 years ago. After surgery, maximum corneal keratometry, mean corneal keratometry, spherical equivalent and uncorrected distance visual acuity were significantly improved at follow-up intervals. Conclusions Transepithelial PTK combined with CXL for corneal ectasia after SMILE may be an effective and safe treatment in the long term