Analysis on the technical detection capacity of radioactive substances in food in China

To analyze the detection capacity of radioactive substances in food in China， and improve the radioactive contamination monitoring system. By studying the distribution of certified institutions and testing items and the results of proficiency assessment， the current situation and deficiencies of the detection capacities were analyzed， and corresponding countermeasures were put forward. The capacity of radioactive material detection in China can better support the operation of the monitoring system， however， the effectiveness and sustainability of testing capacity， the layout of capacity network and the construction of food radioactive pollution monitoring system need to be further improved and strengthened， so as to meet the needs of normal circumstances and rapid response in case of nuclear or radiological emergencies in China

Comparing ATPase activity of ATP-binding cassette subfamily C member 4, lamprey CFTR, and human CFTR using an antimony-phosphomolybdate assay

Introduction: ATP-binding cassette (ABC) transporters use the hydrolysis of ATP to power the active transport of molecules, but paradoxically the cystic fibrosis transmembrane regulator (CFTR, ABCC7) forms an ion channel. We previously showed that ATP-binding cassette subfamily C member 4 (ABCC4) is the closest mammalian paralog to CFTR, compared to other ABC transporters. In addition, Lamprey CFTR (Lp-CFTR) is the oldest known CFTR ortholog and has unique structural and functional features compared to human CFTR (hCFTR). The availability of these evolutionarily distant orthologs gives us the opportunity to study the changes in ATPase activity that may be related to their disparate functions.Methods: We utilized the baculovirus expression system with Sf9 insect cells and made use of the highly sensitive antimony-phosphomolybdate assay for testing the ATPase activity of human ABCC4 (hABCC4), Lp-CFTR, and hCFTR under similar experimental conditions. This assay measures the production of inorganic phosphate (Pi) in the nanomolar range.Results: Crude plasma membranes were purified, and protein concentration, determined semi-quantitatively, of hABCC4, Lp-CFTR, and hCFTR ranged from 0.01 to 0.36 μg/μL. No significant difference in expression level was found although hABCC4 trended toward the highest level. hABCC4 was activated by ATP with the equilibrium constant (Kd) 0.55 ± 0.28 mM (n = 8). Estimated maximum ATPase rate (Vmax) for hABCC4 was about 0.2 nmol/μg/min when the protein was activated with 1 mM ATP at 37°C (n = 7). Estimated maximum ATPase rate for PKA-phosphorylated Lp-CFTR reached about half of hCFTR levels in the same conditions. Vmax for both Lp-CFTR and hCFTR were significantly increased in high PKA conditions compared to low PKA conditions. Maximum intrinsic ATPase rate of hABCC4 in the absence of substrate was twice that of hCFTR when activated in 1 mM ATP.Conclusion: The findings here suggest that while both ABCC4 and hCFTR bear one consensus and one degenerate ATPase site, the hCFTR exhibited a reduced intrinsic ATPase activity. In addition, ATPase activity in the CFTR lineage increased from Lp-CFTR to hCFTR. Finally, the studies pave the way to purify hABCC4, Lp-CFTR, and hCFTR from Sf9 cells for their structural investigation, including by cryo-EM, and for studies of evolution in the ABC transporter superfamily

The Impact of the Low-Carbon Energy Concept and Green Transition on Corporate Behaviour—A Perspective Based on a Contagion Model

With the globalisation of the economy and the increasing interconnectedness of individuals in the financial markets, companies implementing high energy consumption strategies have become more widespread due to the “herding effect” as they become more closely linked for development. In the context of carbon neutrality, the issue of how to reduce the spread of high energy consumption strategies and the issue surrounding the governance of corporate emissions have become a focus of research. This paper uses the improved SEIJRS infectious disease model to investigate the phenomenon of corporate high energy strategy infection, combined with optimal control theory, to provide a reference for governments and regulators to develop reasonable optimal prevention and control strategies

The Impact of the Low-Carbon Energy Concept and Green Transition on Corporate Behaviour—A Perspective Based on a Contagion Model

With the globalisation of the economy and the increasing interconnectedness of individuals in the financial markets, companies implementing high energy consumption strategies have become more widespread due to the “herding effect” as they become more closely linked for development. In the context of carbon neutrality, the issue of how to reduce the spread of high energy consumption strategies and the issue surrounding the governance of corporate emissions have become a focus of research. This paper uses the improved SEIJRS infectious disease model to investigate the phenomenon of corporate high energy strategy infection, combined with optimal control theory, to provide a reference for governments and regulators to develop reasonable optimal prevention and control strategies