Maternal metabolic syndrome affects the progeny's redox balance and increases neuroinflammation with neurodevelopmental and metabolic adverse consequences

Various lifestyle factors, including diet, can impact on redox balance and brain health. Consumption of fructose-sweetened beverages has drastically increased in the last decades and is widely associated with metabolic disease, systemic proinflammatory status and adverse transgenerational effects. To date, the impact of maternal fructose intake in brain redox balance and function of the offspring is less explored. We investigated whether the progeny of mothers with Metabolic Syndrome (MetS), induced by ad libitum consumption of a 20% fructose solution, present any redox alteration in the brain as a consequence of being gestated in a metabolic altered intrauterine environment. Wistar rats were randomly separated into two groups with access to water or fructose (20% w/v in water) for 10 weeks. After MetS was confirmed, dams were mated with control males and continued drinking water or fructose solution during gestation. At postnatal day (PN) 1, a subgroup of offspring of each sex was sacrificed and brains were dissected for oxidative stress and inflammatory status analysis. The developmental milestones and behavioral test were also evaluated (PN3-PN100) in another subgroup of offspring to identify any long-term consequence to being gestated by a dam with MetS. Maternal MetS affects the redox balance and increases neuroinflammation in female offspring at birth. Sexually dimorphic effects were also found on the progeny's acquisition of neurodevelopmental milestones and in their psychiatric, cognitive and metabolic state. Although direct extrapolation of our findings cannot be made to humans, the results presented herein reinforce the necessity of considering the potentially negative effects of fructose-induced MetS prior to, and during pregnancy in offspring’s brain and metabolic physiologyFil: Prado Spalm, Facundo Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Cuervo Sánchez, Marié Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaWorshop on Redox Nutrition and ToxicologyArgentinaUniversidad de Buenos Aires. Facultad de Farmacia y Bioquímic

Rat maternal insulin resistance is associated with abnormal neurobehavioral response in offspring

It is well established that maternal diet and metabolic state during pregnancy contributes to the risk of metabolic disease in offspring. Furthermore, recent epidemiological evidence suggests that gestational factors such as increased maternal obesity and impaired glucose metabolism can likewise affect offspring neurodevelopment, increasing the risk of neuropsychiatric disorders. We aimed to investigate the influence of long-term maternal fructose intake during preconception, gestation and lactation periods on neurobehavioral development of rat offspring. Wistar rats received either 10 % fructose enriched water or regular tap water for 20 weeks before and during gestation and through lactation. On P21, all littermates were separated and housed with ad libitum access to standard food and tap water. Control and fructose-fed mother’s blood samples were collected for biochemical analysis. Offspring behavior was evaluated using open field, social interaction, marble burying and T-Maze task performance tests. Data analyses were carried independently on male and female rats. Dams fed with a 10 % (w/v) beverage containing fructose showed a moderated body weight gain and significant increments in fasting glucose level, oral glucose tolerance test (OGTT) and Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) index. Behavioral evaluation of the offspring revealed that females exposed to maternal fructose intake were prone to have increased number of marble buried and reduced learning performance in a T-maze compared to control-diet offspring. Our findings indicate that chronic maternal metabolic stress induced by a fructose-rich diet during pre- gestational, gestational and lactational periods showed a gender- specific increase in stereotyped repetitive behavior and working memory tasks in the offspring.Fil: Cuervo Sánchez, Marié Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Prado Spalm, Facundo Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Bonaventura, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaReunión anual de Sociedades de Biociencia 2019; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL)Mar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de NanomedicinasAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioThe Histochemical Societ