92 research outputs found
Mycoplasma hyopneumoniae evades phagocytic uptake by porcine alveolar macrophages in vitro
Mycoplasma hyopneumoniae, the agent of porcine enzootic pneumonia (EP), is able to persist in the lung tissue and evade destruction by the host for several weeks. To understand the mechanism of pathogen survival, phagocytic uptake of M. hyopneumoniae by primary porcine alveolar macrophages was investigated. Intracellular location and survival of the pathogen were explored using gentamicin survival assays, flow cytometry and confocal microscopy of M. hyopneumoniae 232 labelled with green fluorescent protein (GFP). Following 1 h and 16 h of co-incubation, few viable M. hyopneumoniae were recovered from inside macrophages. Flow cytometric analysis of macrophages incubated with M. hyopneumoniae expressing GFP indicated that the mycoplasmas became associated with macrophages, but were shown to be extracellular when actin-dependent phagocytosis was blocked with cytochalasin D. Confocal microscopy detected GFP-labelled M. hyopneumoniae inside macrophages and the numbers increased modestly with time of incubation. Neither the addition of porcine serum complement or convalescent serum from EP-recovered pigs was able to enhance engulfment of M. hyopneumoniae. This investigation suggests that M. hyopneumoniae evades significant uptake by porcine alveolar macrophages and this may be a mechanism of immune escape by M. hyopneumoniae in the porcine respiratory tract
Disclinations, dislocations and continuous defects: a reappraisal
Disclinations, first observed in mesomorphic phases, are relevant to a number
of ill-ordered condensed matter media, with continuous symmetries or frustrated
order. They also appear in polycrystals at the edges of grain boundaries. They
are of limited interest in solid single crystals, where, owing to their large
elastic stresses, they mostly appear in close pairs of opposite signs. The
relaxation mechanisms associated with a disclination in its creation, motion,
change of shape, involve an interplay with continuous or quantized dislocations
and/or continuous disclinations. These are attached to the disclinations or are
akin to Nye's dislocation densities, well suited here. The notion of 'extended
Volterra process' takes these relaxation processes into account and covers
different situations where this interplay takes place. These concepts are
illustrated by applications in amorphous solids, mesomorphic phases and
frustrated media in their curved habit space. The powerful topological theory
of line defects only considers defects stable against relaxation processes
compatible with the structure considered. It can be seen as a simplified case
of the approach considered here, well suited for media of high plasticity
or/and complex structures. Topological stability cannot guarantee energetic
stability and sometimes cannot distinguish finer details of structure of
defects.Comment: 72 pages, 36 figure
Muon Physics: A Pillar of the Standard Model
Since its discovery in the 1930s, the muon has played an important role in
our quest to understand the sub-atomic theory of matter. The muon was the first
second-generation standard-model particle to be discovered, and its decay has
provided information on the (Vector -Axial Vector) structure of the weak
interaction, the strength of the weak interaction, G_F, and the conservation of
lepton number (flavor) in muon decay. The muon's anomalous magnetic moment has
played an important role in restricting theories of physics beyond the standard
standard model, where at present there is a 3.4 standard-deviation difference
between the experiment and standard-model theory. Its capture on the atomic
nucleus has provided valuable information on the modification of the weak
current by the strong interaction which is complementary to that obtained from
nuclear beta decay.Comment: 8 pages, 9 figures. Invited paper for the Journal of Physical Society
in Japan (JPSJ), Special Topics Issue "Frontiers of Elementary Particle
Physics, The Standard Model and beyond
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