Habilidades personales y el rendimiento laboral en la unidad de gestión educativa local, Apurímac, 2022

La investigación tiene como objetivo principal determinar la relación entre las habilidades personales y el rendimiento laboral en la UGEL de Apurímac. La investigación fue de tipo aplicada, con un diseño no experimental de corte transversal, con un nivel descriptivo correlacional bajo un enfoque cuantitativo. La muestra del estudio fue de un total de 110 trabajadores, se usaron dos cuestionarios que corresponden a las dos variables de investigación;las cuales sirvieron para recopilar la información necesaria y proceder a realizar elprocesamiento de los datos, aplicando la estadística descriptiva e inferencial. El resultado de la Rho de Spearman fue de 0.753, esto indica que, existe una correlación positiva alta entre las variables de investigación. Se concluyó que una adecuada gestión de habilidades personales de parte de los líderes de la organización se reflejará de manera directa en el rendimiento laboral de ellos mismos y de los trabajadores

Experiences in Teaching Veterinary Public Health across Latin-America and Europe: the SAPUVETNET III Project

Experiences in Teaching Veterinary Public Health across Latin-America and Europe: the SAPUVETNET III Project SAPUVETNET III (n. DCI-LA/2008/75) is the third phase of a series of projects, co-financed under the EU ALFA programme, aimed to support a VPH network constituted by Faculties of Veterinary Medicine of 12 Latin-american and 6 European countries in addition to various collaborating institutions/organizations both at national and international level (http://www.sapuvetnet.org). The project envisages the development and the implementation of a common VPH curriculum, through the use of innovative teaching methods, mainly based on problem solving approach. The authors present here some teaching material developed by the project as an example of new strategies/approach for teaching VPH: case studies, videos and self-learning programme on meat inspection/food hygiene, an Interactive Manual on VPH, as well as e-conferences on upcoming VPH issues. Project partners use a mail-list and distance learning platforms (e.g. Moodle, Colibri) to organize teaching activities. A Journal, “Una Salud/One Health/Uma Saúde”, is also published and distributed both as hard copy or .pdf through the web. Didactic tools produced by the SAPUVETNET projects have been and/or are being tested and used by the partner faculties and other teaching institutions, both for under and post-graduate courses. Teaching material by SAPUVETNET is distributed according to Creative Common criteria and policy (http://creativecommons.org/). It can be freely circulated and distributed, it can be used for distance learning and can be modified/adapted to the local context at each country/geographical area, even outside Latin-America and Europe. Teaching products produced under the SAPUVETNET projects are available at the URL http://www.sapuvetnet.org, or can be obtained from the project co-ordinator(s) and/or the contact persons at the partner Faculties/Universities. The Authors would like to acknowledge all participants/collaborators/partners of the current and previous SAPUVETNET projects who greatly contributed –in different moments and at different levels- to the development of the teaching tools herein described

Emergence of pyridoxal phosphorylation through a promiscuous ancestor during the evolution of hydroxymethyl pyrimidine kinases

Artículo de publicación ISIIn the family of ATP-dependent vitamin kinases, several bifunctional enzymes that phosphorylate hydroxymethyl pyrimidine (HMP) and pyridoxal (PL) have been described besides enzymes specific towards HMP. To determine how bifunctionality emerged, we reconstructed the sequence of three ancestors of HMP kinases, experimentally resurrected, and assayed the enzymatic activity of their last common ancestor. The latter has 8-fold higher specificity for HMP due to a glutamine residue (Gln44) that is a key determinant of the specificity towards HMP, although it is capable of phosphorylating both substrates. These results show how a specific enzyme with catalytic promiscuity gave rise to current bifunctional enzymes.This work was supported by Fondo Nacional de Desarrollo Cientifico y Tecnologico (Fondecyt, Chile) Grant 1110137

Regulatory T cells and IgE expression in duodenal mucosa of Strongyloides stercoralis and human T lymphotropic virus type 1 co-infected patients.

BackgroundStrongyloides stercoralis is an intestinal nematode unique in its ability to replicate in the human host, allowing ongoing cycles of autoinfection, persisting for decades within the same host. Although usually asymptomatic, overwhelming infections can occur in Strongyloides and HTLV-1 co-infected individuals (SS/HTLV-1). Regulatory T cells (Tregs) are able to blunt specific Th2 responses necessary to control the parasite. We previously reported that peripheral blood Tregs are increased in SS/HTLV-1 and correlate with low Th2 responses. We hypothesized that Tregs are also increased at the site of infection in duodenal mucosa.MethodsParaffin embedded duodenal biopsies were obtained from 10 SS/HTLV-1 patients, 3 controls with non-parasitic chronic duodenitis, and 2 healthy controls. Immunohistochemistry was performed using monoclonal antibodies against human CD3, CD8, IgE and FoxP3. The number of cells were counted using a conventional light microscope. The number of CD3+, CD8+, FoxP3+ and IgE positive cells per 0.35 mm2 was measured using ImagePro Plus software comparing areas adjacent or distant from parasite material.ResultsIn patients with SS/HTLV-1, T lymphocyte counts and CD8+ cells were lower in areas adjacent to the parasite compared to non-adjacent areas (CD3+: adjacent: 6.5 [Interquartile range (IQR: 2.8-12.3)]; non-adjacent: 24.5 [IQR: 20.9-34.4]; Mann-Whitney p = 0.0003; CD8+: adjacent: 4.5 [IQR: 2.3-11.8]; non-adjacent: 21 [IQR: 15.3-42.9]; Mann-Whitney p = 0.0011). Tregs cells in the intestines (FoxP3+ expressing cells) were increased in patients with SS/HTLV-1 compared with patients with chronic duodenitis (SS/HTLV-1: 1.5 [IQR: 0.7-2.3]; duodenitis controls: 0 [range 0-0.7]; healthy controls: 0; Mann-Whitney p = 0.034). There was also a trend towards fewer eosinophils adjacent to the parasites. Among SS/HTLV-1 patients the number of IgE expressing cells was increased for in areas not adjacent to the parasite compared to non-adjacent areas (ANOVA, p = 0.001).ConclusionsOur data shows increased Treg cell numbers localized adjacent to the parasites in the duodenum SS/HTLV-1 patients. In addition, other T lymphocytes and IgE expressing cells were decreased adjacent to the parasites, suggesting an important role for Tregs in down-regulating local parasite effector responses

Inferring directed networks using a rank-based connectivity measure

Inferring the topology of a network using the knowledge of the signals of each of the interacting units is key to understanding real-world systems. One way to address this problem is using data- driven methods like cross-correlation or mutual information. However, these measures lack the ability to distinguish the direction of coupling. Here, we use a rank-based nonlinear interdependence measure originally developed for pairs of signals. This measure not only allows one to measure the strength but also the direction of the coupling. Our results for a system of coupled Lorenz dynamics show that we are able to consistently infer the underlying network for a subrange of the coupling strength and link density. Furthermore, we report that the addition of dynamical noise can benefit the reconstruction. Finally, we show an application to multichannel electroencephalographic recordings from an epilepsy patient.This work was funded by the EU via H2020 Marie SklodowskaCurie project COSMOS, grant no. 642563 (M.G.L., I.M., Z.L., and R.G.A). R.G.A. and C.G.B.M. acknowledge funding from the Spanish Ministry of Econ- omy and Competitiveness (Grant FIS2014-54177-R) and the CERCA Programme of the Generalitat de Catalunya. C.G.B.M. acknowledges the support by the Spanish Ministry of Economy and Competitiveness under the Maria de Maeztu Units of Excellence Programme (MDM-2015-0502). Z.L. acknowledges funding from the Slovenian Re- search Agency via program Complex Networks P1-0383 and project J5-8236

Inferring directed networks using a rank-based connectivity measure

Inferring the topology of a network using the knowledge of the signals of each of the interacting units is key to understanding real-world systems. One way to address this problem is using data- driven methods like cross-correlation or mutual information. However, these measures lack the ability to distinguish the direction of coupling. Here, we use a rank-based nonlinear interdependence measure originally developed for pairs of signals. This measure not only allows one to measure the strength but also the direction of the coupling. Our results for a system of coupled Lorenz dynamics show that we are able to consistently infer the underlying network for a subrange of the coupling strength and link density. Furthermore, we report that the addition of dynamical noise can benefit the reconstruction. Finally, we show an application to multichannel electroencephalographic recordings from an epilepsy patient.This work was funded by the EU via H2020 Marie SklodowskaCurie project COSMOS, grant no. 642563 (M.G.L., I.M., Z.L., and R.G.A). R.G.A. and C.G.B.M. acknowledge funding from the Spanish Ministry of Econ- omy and Competitiveness (Grant FIS2014-54177-R) and the CERCA Programme of the Generalitat de Catalunya. C.G.B.M. acknowledges the support by the Spanish Ministry of Economy and Competitiveness under the Maria de Maeztu Units of Excellence Programme (MDM-2015-0502). Z.L. acknowledges funding from the Slovenian Re- search Agency via program Complex Networks P1-0383 and project J5-8236