Congenital Anomalies of Urinary Tract and Anomalies of Fetal Genitalia

Congenital anomalies of the kidney, urinary tract and genitalia anomalies are among the most frequent types of congenital malformations. Many can be diagnosed by means of ultrasound examination during pregnancy. Some will be discovered after birth. Kidney and urinary malformations represent 20% of all birth defects, appearing in 3–7 cases at 1000 live births. Environmental factors (maternal diabetes or intrauterine exposure to angiotensin-converting enzyme inhibitors) and genetic factors (inherited types of diseases) seem to be among causes that lead to the disturbance of normal nephrogenesis and generate anomalies of the reno-urinary tract. It is very important to diagnose and differentiate between the abnormalities incompatible with life and those that are asymptomatic in the newborn. The former requires interruption of pregnancy, whereas the latter could lead to saving the renal function if diagnosed antenatally. In many cases, the congenital anomalies of the urinary and genital tract may remain asymptomatic for a long time, even up until adulthood, and can be at times the only manifestation of a complex systemic disease. Some can manifest in more than one member in the family. This is the reason why the accurate genetic characterization is needed; it can help give not only the patient but also her family the appropriate genetic counseling, and also, in some cases, the management may prevent severe complications

A Retrospective Narrative Mini-Review Regarding the Seminal Microbiota in Infertile Male

Background: Infertility is a global burden that affects both sexes with the male component remaining as an explored yet crucial research field that might offer novel evidence. Material and Methods: The present narrative mini-review aims to summarize all existing literature regarding the composition of the seminal microflora in infertile men. We performed searches in PubMed/Medline, ISI Web of Knowledge, Scopus, and ScienceDirect between 2018 and 2022 using a combination of keywords. Results: A total of n = 33 studies met the eligibility criteria and were further considered. From this, n = 14 were conducted on human patients, n = 3 on zebrafish (Danio rerio), n = 5 on rats, and n = 11 on mice. In twenty-five out of thirty-three papers, the authors sequenced the 16S rRNA; situations occurred where researchers focused on standard laboratory protocols. Lactobacillus and Bifidobacterium are widely recognized as putative beneficial lactic bacteria. These two entities are capable of restoring the host’s eubiosis to some extent, blocking pathogens’ proliferation and endotoxins, and even alleviating specific patterns encountered in disease(s) (e.g., obesity, type 1 diabetes) due to prolonged exposure to toxicants in adults or from a developmental stage. Over the years, distinct approaches have been perfected, such as the transfer of feces between two species or conventional rudimentary products with proven efficiency. Conclusions: The seminal microflora is decisive and able to modulate psychological and physiological responses. Each individual possesses a personalized microbial profile further shaped by exogenous factors, regardless of sex and species

ABOUT THREE-DIMENSIONAL MODELS OF OSTEOSYNTHESIS SYSTEMS

Implantology is based on the failure of orthopedic treatment, or cases where orthopedic treatment is incapable of reducing or maintaining, for example, fracture of the femoral neck with movement. The advantage of centromedular fixation is that it allows the bone to physically load as much as possible, minimizing the risk of implant failure. The drawbacks of conventional nails have been eliminated with the introduction of the centromedular stem locking system. The purpose of this study was to obtain several virtual biomechanical systems on which to study several types of tibial fractures and several osteosynthesis systems. Starting from the tomographic images of the tibia, a virtual model of this bone component was developed. This model was "finalized" and modified using certain Geomagic techniques and then imported into SolidWorks. The osteosynthesis element used was the rigid classical nail with orthopedic screws which was modeled using the direct observation and measurement method. It is intended that these virtual components to be used to make orthopedic virtual osteosynthesis systems that are then analyzed in Ansys with the finite element method

THE MODEL OF A PROSTHETIC SYSTEM FOR HUMAN KNEE JOINT

Obesity, sedentarism, and other disadvantages of the modern age have led to the emergence of unknown pathological situations a few centuries before. At the same time, the development of medicine, orthopaedic surgery has led to finding ways to solve these situations. The present paper proves that the use of high-tech methods used in engineering can generate solutions in the field of orthopaedics both for obtaining new prothesis models, but also by creating virtual environments for tests. Several CAD techniques and techniques have been explored, coupled with medical imaging, can provide exciting solutions for obtaining virtual environments that can allow "in vitro" analysis of various normal, pathological or prosthetic joints. In this paper we have detailed how to obtain a prosthetic knee joint in a parameterized environment. This joint was tested virtually. In the final of the paper, main conclusions were made

THREE-DIMENSIONAL MODEL OF A HIP PROSTHESIS

Serious pathological problems of the hip joint can be solved surgically, in some cases by joint prosthesis. For this reason, it is very important to be able to shape, in a first phase, the components of the prosthesis, which can then be tested virtually. For this purpose, the components of the prosthesis with fixation pins were firstly scanned three-dimensionally. This operation resulted in a model of a "cloud of points" which was subsequently transformed into virtual surfaces, then into virtual solids. These virtually solid components were assembled in a parameterized virtual environment. This prosthesis can be inserted into a biomechanical model of the hip joint and tested using the finite element method. At the end of the paper important conclusions were drawn

MONOAMINE OXIDASE INHIBITION IMPROVES VASCULAR FUNCTION IN MAMMARY ARTERIES FROM NON-DIABETIC AND DIABETIC PATIENTS WITH CORONARY HEART DISEASE

Monoamine oxidases (MAOs) are mitochondrial enzymes with 2 isoforms that have emerged as important contributors to cardiovascular oxidative stress via the constant generation of hydrogen peroxide. The present study was purported to assess whether MAO-derived H2O2 contributes to the endothelial dysfunction in mammary arteries harvested from coronary heart disease patients with/without diabetes mellitus subjected to coronary artery bypass grafting. To this aim the effects of MAO inhibition on vascular contractility to phenylephrine and endothelial-dependent relaxation (EDR) in response to acetylcholine were studied in vascular segments. Clorgyline (irreversible MAO A inhibitor), selegiline (irreversible MAO B inhibitor), and moclobemide (reversible MAO A inhibitor) were applied in the organ bath (10Âľmol/L). MAO expression was assessed by immunohistochemistry. We found a constant impairment of EDR that has been significantly attenuated in the presence of the MAO A and B inhibitors in both groups of coronary heart disease patients. MAO B was the dominant isoform in all human diseased vessels. In conclusion, in vitro inhibition of MAO significantly improved endothelium-dependent relaxation in human mammary arteries, regardless the presence of diabetes. These data suggest that MAO inhibitors might be useful in restoring endothelial response in clinical conditions associated with increased oxidative stress, such as coronary artery disease and diabetes.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Unraveling the Efficacy of Therapeutic Interventions for Short Cervix: Insights from a Retrospective Study for Improved Clinical Management

Background and Objectives: Preterm birth (PTB) is associated with important neonatal mortality and morbidity. The aim of this study was to retrospectively evaluate the average treatment effects on the treated and the efficacity of various therapeutic interventions for PTB in a cohort of patients with singleton pregnancies and short cervical lengths. Materials and Methods: This observational retrospective study included 1146 singleton pregnancies at risk of PTB that were segregated into the following groups: intravaginal progesterone (group 1), Arabin pessary (group 2), McDonald cerclage (group 3), intravaginal progesterone and Arabin pessary (group 4), and intravaginal progesterone and cerclage (group 5). Their treatment effects were evaluated and compared. Results: All evaluated therapeutic interventions significantly reduced the occurrence of late and early preterm births. The risk of late and early PTB was lowered for those pregnant patients who received progesterone and pessaries or progesterone and cerclage in comparison with those who received only progesterone. The extremely PTB risk of occurrence was significantly lowered only by the administration of progesterone in association with cervical cerclage in comparison with progesterone monotherapy. Conclusions: The combined therapeutic interventions had the highest efficacy in preventing preterm birth. An individualized evaluation is needed to establish the best therapeutic approach in particular cases

Pregnancy Outcomes, Immunophenotyping and Immunohistochemical Findings in a Cohort of Pregnant Patients with COVID-19—A Prospective Study

(1) Background: SARS-CoV-2 infection during pregnancy could determine important maternal and fetal complications. We aimed to prospectively assess placental immunohistochemical changes, immunophenotyping alterations, and pregnancy outcomes in a cohort of patients with COVID-19; (2) Methods: 52 pregnant patients admitted to a tertiary maternity center between October 2020 and November 2021 were segregated into two equal groups, depending on the presence of SARS-CoV-2 infection. Blood samples, fragments of umbilical cord, amniotic membranes, and placental along with clinical data were collected. Descriptive statistics and a conditional logistic regression model were used for data analysis; (3) Results: Adverse pregnancy outcomes such as preterm labor and neonatal intensive care unit admission did not significantly differ between groups. The immunophenotyping analysis indicated that patients with moderate–severe forms of COVID-19 had a significantly reduced population of T lymphocytes, CD4+ T cells, CD8+ T cells (only numeric), CD4+/CD8+ index, B lymphocytes, and natural killer (NK) cells. Our immunohistochemistry analysis of tissue samples failed to demonstrate positivity for CD19, CD3, CD4, CD8, and CD56 markers; (4) Conclusions: Immunophenotyping analysis could be useful for risk stratification of pregnant patients, while further studies are needed to determine the extent of immunological decidual response in patients with various forms of COVID-19