10 research outputs found
Influence of batch or fed-batch growth on Staphylococcus epidermidis biofilm formation
Aims: To make a quantitative evaluation of the differences in biofilm formation by Staphylococcus epidermidis using
batch and fed-batch growth systems and to correlate this with production of the major biofilm polysaccharide,
poly-N-acetyl glucosamine (PNAG).
Methods and Results: Dry weight measurements of biofilms formed in batch and fed-batch conditions were
compared with haemagglutination titres, which measure the amount of PNAG produced. Strains grown in batch
systems developed less biofilm than when grown in fed-batch systems. A good correlation was found between the
amount of biofilm formed in fed-batch systems and the haemagglutination titres.
Conclusions: Differences in biofilm formation and PNAG production by S. epidermidis are dependent on the
availability of nutrients, with higher availability correlating with more biofilm and PNAG production.
Significance of and Impact of the Study: Comparisons of the formation of biofilms by S. epidermidis are
dependent on choosing an appropriate biofilm growth system. Comparability or disparity of conclusions among
different investigations will be strongly influenced by which mode S. epidermidis biofilms are formed.NIH - grant AI 46706.FundaĆ§Ć£o para a CiĆŖncia e a Tecnologia (FCT) ā Programa Operacional āCiĆŖncia, Tecnologia, InovaĆ§Ć£oā (POCTI) - POCTI/ESP/42688/2001, SFRH/BD/8676/2002
A Staphylococcus aureus ypfP mutant with strongly reduced lipoteichoic acid (LTA) content: LTA governs bacterial surface properties and autolysin activity
Many Gram-positive bacteria produce lipoteichoic acid (LTA) polymers whose physiological roles have remained a matter of debate because of the lack of LTA-deficient mutants. The ypfP gene responsible for biosynthesis of a glycolipid found in LTA was deleted in Staphylococcus aureus SA113, causing 87% reduction of the LTA content. Mass spectrometry and nuclear magnetic resonance spectroscopy revealed that the mutant LTA contained a diacylglycerol anchor instead of the glycolipid, whereas the remaining part was similar to the wild-type polymer except that it was shorter. The LTA mutant strain revealed no major changes in patterns of cell wall proteins or autolytic enzymes compared with the parental strain indicating that LTA may be less important in S. aureus protein attachment than previously thought. However, the autolytic activity of the mutant was strongly reduced demonstrating a role of LTA in controlling autolysin activity. Moreover, the hydrophobicity of the LTA mutant was altered and its ability to form biofilms on plastic was completely abrogated indicating a profound impact of LTA on physicochemical properties of bacterial surfaces. We propose to consider LTA and its biosynthetic enzymes as targets for new antibiofilm strategies