72 research outputs found
Immunomagnetic t-lymphocyte depletion (ITLD) of rat bone marrow using OX-19 monoclonal antibody
Graft versus host disease (GVHD) may be abrogated and host survival prolonged by in vitro depletion of T lymphocytes from bone marrow (BM) prior to allotransplantation. Using a mouse anti-rat pan T-lymphocyte monoclonal antibody (0×19) bound to monosized, magnetic, polymer beads, T lymphocytes were removed in vitro from normal bone marrow. The removal of the T lymphocytes was confirmed by flow cytometry. Injection of the T-lymphocyte-depleted bone marrow into fully allogeneic rats prevents the induction of GVHD and prolongs host survival. A highly efficient technique of T-lymphocyte depletion using rat bone marrow is described. It involves the binding of OX-19, a MoAb directed against all rat thy-mocytes and mature peripheral T lymphocytes, to monosized, magnetic polymer spheres. Magnetic separation of T lymphocytes after mixing the allogeneic bone marrow with the bead/OX-19 complex provides for a simple, rapid depletion of T lymphocytes from the bone marrow. In vitro studies using flow cytometry and the prevention of GVHD in a fully allogeneic rat bone marrow model have been used to demonstrate the effectiveness of the depletion procedure. © 1989 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted
An accurate nucleon-nucleon potential with charge-independence breaking
We present a new high-quality nucleon-nucleon potential with explicit charge
dependence and charge asymmetry, which we designate Argonne . The model
has a charge-independent part with fourteen operator components that is an
updated version of the Argonne potential. Three additional
charge-dependent and one charge-asymmetric operators are added, along with a
complete electromagnetic interaction. The potential has been fit directly to
the Nijmegen and scattering data base, low-energy scattering
parameters, and deuteron binding energy. With 40 adjustable parameters it gives
a per datum of 1.09 for 4301 and data in the range 0--350
MeV.Comment: 36 pages, PHY-7742-TH-9
Effects of the application of sewage sludge and petrochemical residue in maize culture as source of micro-nutrients on soils of Paraná state
Sequential Sampling Plan based on the Informational Energy for an Exponential Population
GENETIC STUDIES IN INBRED RATS VII. TENTATIVE MODEL FOR THE MAJOR HISTOCOMPATIBILITY COMPLEX
COMPARISON OF THE HAPLOTYPES OF THE MAJOR HISTOCOMPATIBILITY COMPLEX IN THE RAT IV. THE SIX ORIGINAL AG-B HAPLOTYPES
Search for correlations between prolate-shape collective and oblate-shape non collective nuclear rotation: High spin states in Yb
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