Immunogold Localization of Intra- and Extra-Cellular Proteins and Polysaccharides of Plant Cells

This paper illustrates post-embedding immunogold labelling of protein and polysaccharide molecules of plant cells. For EM studies, one is restricted (for most plant cells) to the post-embedding approach because the surrounding cell wall prevents access of antibodies (and secondary gold-tagged markers) to internal sites. The large size of many plant cells also does not lend itself to diffusional entry of antibodies. The molecules localized include seed storage proteins that are large and present in major quantities, a smaller less abundant, water soluble albumin, an oxygen-binding protein, components of the photosynthetic electron transport chain, and complex sugars from the cell wall. A range of preparative procedures and embedding plastics are used

Undergraduate Course in Environmental Design and Manufacturing

This paper is a progress report on the development of an undergraduate course in environmental design and manufacturing. The importance of the topic is clear from National Academy of Engineering statements regarding the need to incorporate content on sustainable technology. The multidisciplinary team includes academic faculty from engineering, science, liberal studies, and business in collaboration with industry partners. The team\u27s approach is to create a case-based course by adapting existing educational modules from Ford Motor Company. The group has received National Science Foundation funding for this project (DUE-0511322), and the project is currently in its beginning phases

Barriers to integrating direct oral anticoagulants into anticoagulation clinic care: A mixedâ methods study

BackgroundOutpatient anticoagulation clinics were initially developed to care for patients taking vitamin K antagonists such as warfarin. There has not been a systematic evaluation of the barriers and facilitators to integrating direct oral anticoagulant (DOAC) care into outpatient anticoagulation clinics.MethodsWe performed a mixed methods study consisting of an online survey of anticoagulation clinic providers and semiâ structured interviews with anticoagulation clinic leaders and managers between March and May of 2017. Interviews were transcribed and coded, exploring for themes around barriers and facilitators to DOAC care within anticoagulation clinics. Survey questions pertaining to the specific themes identified in the interviews were analyzed using summary statistics.ResultsSurvey responses were collected from 159 unique anticoagulation clinics and 20 semiâ structured interviews were conducted. Three primary barriers to DOAC care in the anticoagulation clinic were described by the interviewees: (a) a lack of provider awareness for ongoing monitoring and services provided by the anticoagulation clinic; (b) financial challenges to providing care to DOAC patients in an anticoagulation clinic model; and (c) clinical knowledge versus scope of care by the anticoagulation staff. These themes linked to three key areas of variation, including: (a) the size and hospital affiliation of the anticoagulation clinic; (b) the use of faceâ toâ face versus telephoneâ based care; and (c) the use of nurses or pharmacists in the anticoagulation clinic.ConclusionsAnticoagulation clinics in the United States experience important barriers to integrating DOAC care. These barriers vary based on the clinic size, model for warfarin care, and staff credentials (nursing or pharmacy).Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/147845/1/rth212157.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/147845/2/rth212157_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/147845/3/rth212157-sup-0001-Supinfo.pd

AHRQ series on complex intervention systematic reviews-paper 5: advanced analytic methods.

BACKGROUND AND OBJECTIVE: Advanced analytic methods for synthesizing evidence about complex interventions continue to be developed. In this paper, we emphasize that the specific research question posed in the review should be used as a guide for choosing the appropriate analytic method. METHODS: We present advanced analytic approaches that address four common questions that guide reviews of complex interventions: (1) How effective is the intervention? (2) For whom does the intervention work and in what contexts? (3) What happens when the intervention is implemented? and (4) What decisions are possible given the results of the synthesis? CONCLUSION: The analytic approaches presented in this paper are particularly useful when each primary study differs in components, mechanisms of action, context, implementation, timing, and many other domains

Analysis of genetically driven alternative splicing identifies FBXO38 as a novel COPD susceptibility gene

While many disease-associated single nucleotide polymorphisms (SNPs) are associated with gene expression (expression quantitative trait loci, eQTLs), a large proportion of complex disease genome-wide association study (GWAS) variants are of unknown function. Some of these SNPs may contribute to disease by regulating gene splicing. Here, we investigate whether SNPs that are associated with alternative splicing (splice QTL or sQTL) can identify novel functions for existing GWAS variants or suggest new associated variants in chronic obstructive pulmonary disease (COPD). RNA sequencing was performed on whole blood from 376 subjects from the COPDGene Study. Using linear models, we identified 561,060 unique sQTL SNPs associated with 30,333 splice sites corresponding to 6,419 unique genes. Similarly, 708,928 unique eQTL SNPs involving 15,913 genes were detected at 10% FDR. While there is overlap between sQTLs and eQTLs, 55.3% of sQTLs are not eQTLs. Co-localization analysis revealed that 7 out of 21 loci associated with COPD (p</p

Prospectus, September 23, 1974

ALL SET FOR STUGO ELECTION; Trustees Approve Budget For \u2774-\u2775, Partial Figures On Page 12; 16 Candidates On Ballot For 1st \u2774 Election; PC Counselors Offer More Than Counseling; Attention: Transfer Students; mercy for who?; The Short Circuit; The Kaleidoscope; Letters; Point-Counterpoint: How Do You View America\u27s Future?; Here Are Candidates\u27 Platforms; State House Candidates To Debate Here; Friends; Untitled; Lit. 1.: A Secret Something, Untitled, Rebirth, Everyone\u27s looking for...; \u27DIRT\u27 Comes Off Clean; Really Raunchy Record Review: Eric Clapton, 461 Ocean Boulevard; Meeting For Prospective Debators; Classified Ads; Books: All The President\u27s Men; Republicans To Have Coffee Wednesday; Phi Beta Lambda Meetings Scheduled; APO Chapter Formed Here; In The Dark: That\u27s Entertainment ; Jock Talk; Harriers Perform Well, Abbey Pleased; IM Football Starts Play On 25th; Howell First \u27Fast Freddy\u27; Bowling Bulletin Board; Watching A Counselor At Work; Registration Drive; Cross Country Schedule; Last Chance For Girls IM Football; Fast Freddy\u27s Football Forecast; Callboard; Parkland Events; Summary Of Approved Budget; Vets Elect Officers; A Column By And For Women; Fashion Forecasthttps://spark.parkland.edu/prospectus_1974/1009/thumbnail.jp