Identification of genes involved in Cd tolerance in Arabidopsis halleri

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Identification of genes involved in Cd hypertolerance by using crosses between Arabidopsis halleri and Arabidopsis lyrata ssp. petraea

Book of abstractinfo:eu-repo/semantics/publishe

Comparative cDNA-AFLP analysis of roots from crosses between Arabidopsis halleri, a Zn, Cd hyperaccumulator and Arabidopsis petraea, a non-tolerant, non-accumulator relative

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Transcriptome analysis of roots from crosses between Arabidopsis halleri ,a Zn, Cd hyperaccumulator and Arabidopsis petraea, a non-tolerant, non-accumulator relative

Book of Abstractsinfo:eu-repo/semantics/publishe

Identification of genes involved in Cd hypertolerance by using crosses between A. halleri and A. lyrata ssp. petraea

info:eu-repo/semantics/publishe

Hardware-in-the-loop simulation applied to protection devices testing

International audienceCircuit breakers are ubiquitous elements used for ensuring safe operation of low-voltage power systems. Nowadays the loads supplied by the power systems migrate towards exhibiting significant strong nonlinear behavior accompanied by inrush currents or high-order harmonics that can cause a false tripping of circuit breakers. It is thus necessary to study the interaction between these new loads and the protection devices in order to fully assess their compatibility. Following this context, this paper deals with a new test rig dedicated to protection switching devices, which is based on the hardware-in-the-loop real-time simulation concept. The proposed approach is focused on replicating the tripping conditions of a real circuit breaker coupled to a power system emulated by means of a real-time simulator. The software part of this simulator adds flexibility to the test rig as the circuit configurations and the operating scenarios may conveniently be adapted at lowest cost. The control of the interface between the software simulator and the hardware under test, as well as the closed-loop stability issues, has been thoroughly approached. Experiments carried out on this test rig show the effectiveness of this new test prototyping concept in characterizing real circuit breakers behavior when they protect lighting circuits

Protection Devices Testing based on Power-Hardware-in-the-Loop Simulation

International audienc

Combined treatments in hepatocellular carcinoma: Time to put them in the guidelines?

The time for battling cancer has never been more suitable than nowadays and fortunately against hepatocellular carcinoma (HCC) we do have a far-reaching arsenal. Moreover, because liver cancer comprises a plethora of stages-from very early to advanced disease and with many treatment options–from surgery to immunotherapy trials–it leaves the clinician a wide range of options. The scope of our review is to throw light on combination treatments that seem to be beyond guidelines and to highlight these using evidence-based analysis of the most frequently used combination therapies, discussing their advantages and flaws in comparison to the current standard of care. One particular combination therapy seems to be in the forefront: Transarterial chemoembolization plus ablation for medium-size non-resectable HCC (3-5 cm), which is currently at the frontier between Barcelona Clinic Liver Cancer classification A and B. Not only does it improve the outcome in contrast to each individual therapy, but it also seems to have similar results to surgery. Also, the abundance of immune checkpoint inhibitors that have appeared lately in clinical trials are bringing promising results against HCC. Although the path of combination therapies in HCC is still filled with uncertainty and caveats, in the following years the hepatology and oncology fields could witness an HCC guideline revolution

Transcriptome analysis by cDNA-AFLP on Suillus luteus Cd-tolerant and Cd-sensitive isolates

The ectomycorrhizal basidiomycete Suillus luteus (L.Fr.), a typical pioneer species which associates with young pine trees colonizing disturbed sites, is a common root symbiont found at heavy metal contaminated sites. Three Cd-sensitive and three Cd-tolerant isolates of S. luteus, isolated respectively from non-polluted and a heavy metal-polluted site in Limburg (Belgium), were used for a transcriptomic analysis. We identified differentially expressed genes by cDNA-AFLP analysis. The possible roles of some of the encoded proteins in heavy metal (Cd) accumulation and tolerance are discussed. Despite the high conservation of coding sequences in S. luteus, a large intraspecific variation in the transcript profiles was observed. This variation was as large in Cd-tolerant as in sensitive isolates and may help this pioneer species to adapt to novel environments.info:eu-repo/semantics/publishe

Comparative cDNA-AFLP analysis of Cd-tolerant and -sensitive genotypes derived from crosses between the Cd hyperaccumulator Arabidopsis halleri and Arabidopsis lyrata ssp. petraea.

Cadmium (Cd) tolerance seems to be a constitutive species-level trait in Arabidopsis halleri. In order to identify genes potentially implicated in Cd tolerance, a backcross (BC1) segregating population was produced from crosses between A. halleri ssp. halleri and its closest non-tolerant relative A. lyrata ssp. petraea. The most sensitive and tolerant genotypes of the BC1 were analysed on a transcriptome-wide scale by cDNA-amplified fragment length polymorphism (AFLP). A hundred and thirty-four genes expressed more in the root of tolerant genotypes than in sensitive genotypes were identified. Most of the identified genes showed no regulation in their expression when exposed to Cd in a hydroponic culture medium and belonged to diverse functional classes, including reactive oxygen species (ROS) detoxification, cellular repair, metal sequestration, water transport, signal transduction, transcription regulation, and protein degradation, which are discussed.Comparative StudyJournal ArticleResearch Support, Non-U.S. Gov'tinfo:eu-repo/semantics/publishe