Conformational substates and motions in myoglobin. External influences on structure and dynamics

Myoglobin, a simppe dioxygen-storage protein, is a good laboratory for the investigation of the connection between protein structure, dynamics, and function. Fourier-transform infrared spectroscopy on carbon-monoxymyoglobin (MbCO) shows three major CO bands. These bands are excellent probes for the investigation of the structure-function relationship. They have different CO binding kinetics and their CO dipoles form different angles with respect to the heme normal, implying that MbCO exists in three major conformational substates, A0, A1, and A3. The entropies and enthalpies of these substates depend on temperature above approximately 180 K and are influenced by pH, solvent, and pressure. These results suggest that even a protein as simple as Mb can assume a small number of clearly different structures that perform the same function, but with different rates. Moreover, protein structure and dynamics depend strongly on the interaction of the protein with its environment

Model-based optimization of the operation of the coke calcining kiln

A mathematical model was recently developed to simulate the calcination process of regular petroleum coke suitable for aluminum industry applications. The model is made of 14 ordinary differential equations describing energy and mass conservation in the gas and in the coke bed, and complemented by correlations and algebraic equations. It calculates temperature and concentration profiles in the kiln, and also yields other information important to kiln operation, such as calcined coke recovery factor and coke loss through the generation of dust. In this paper it is demonstrated that the model is an efficient tool for the optimization of kiln operation. The model is used to study the effect of key control variables upon kiln operation and productivity. Further, it is shown that higher kiln productivity may be obtained with optimized kiln control and without loss of satisfactory kiln operating condition

Transcription Profiling of Candida albicans Cells Undergoing the Yeast-to-Hyphal Transition

The ability of the pathogenic fungus Candida albicans to switch from a yeast to a hyphal morphology in response to external signals is implicated in its pathogenicity. We used glass DNA microarrays to investigate the transcription profiles of 6333 predicted ORFs in cells undergoing this transition and their responses to changes in temperature and culture medium. We have identified several genes whose transcriptional profiles are similar to those of known virulence factors that are modulated by the switch to hyphal growth caused by addition of serum and a 37°C growth temperature. Time course analysis of this transition identified transcripts that are induced before germ tube initiation and shut off later in the developmental process. A strain deleted for the Efg1p and Cph1p transcription factors is defective in hyphae formation, and its response to serum and increased temperature is almost identical to the response of a wild-type strain grown at 37°C in the absence of serum. Thus Efg1p and Cph1p are needed for the activation of the transcriptional program that is induced by the presence of serum

Structural brain alterations in major depression: findings from the ENIGMA Major Depressive Disorder Working Group

Stress-related psychiatric disorders across the life spa