Performances agronomiques des amendements a base de biochar en milieu paysan a l’ouest du Burkina Faso

Au Burkina Faso, la baisse de la fertilité des sols représente une contrainte importante pour la durabilité de l’agriculture. Dans cette étude, l’influence du compost, du biochar et du co-compost au biochar sur les propriétés chimiques du sol, le rendement du cotonnier et du maïs a été évaluée sur un lixisol endoplinthique en milieu paysan. Le dispositif était en blocs randomisés comportant trois traitements en quatre répétitions : T= compost + NPK + Urée, T2 = biochar pristine + NPK + urée, et T3=co-compost au biochar + NPK + urée. La dose d’apport des amendements était de 2,5t.ha-1.an-1 pendant deux ans, associée à la dose recommandée d’engrais minéraux (coton : NPK = 150 kg.ha-1 et urée = 50 kg.ha-1 ; maïs : NPK = 200 kg.ha-1 et urée = 100 kg.ha-1). L’adjonction du biochar pendant le compostage a augmenté les teneurs en azote total, magnésium total et phosphore total de l’amendement obtenu, respectivement de 48 %, 64 % et 68 % comparativement au compost. Même si les paramètres physico-chimiques du sol, deux ans après, n’ont pas été améliorés, une augmentation significative du rendement du coton graine de 14 % (biochar) et 19 % (co-compost au biochar) comparativement au compost a été observée la troisième année. Les amendements à base de biochar peuvent être suggérés aux producteurs pour l’amélioration du rendement des cultures à moyen terme.   English title: Agronomic Performance of Biochar-Based Amendments in Farmers’ environment in western Burkina Faso In Burkina Faso, declining soil fertility is a major constraint to agricultural productivity and sustainability. In the present study, compost, biochar and co-composted biochar were applied to endoplinthic lixisol, and the effects on soil physicochemical properties, cotton and maize yield over three  years (i.e., three cropping seasons) were investigated. The trial was a completely randomized block design included three treatments and four  repetitions: T1= compost + NPK + Urea, T2= pristine biochar + NPK + Urea and T3= co-composted biochar + NPK + Urea. The amendments rate were  2.5 t.ha-1 each year (2018 and 2019) combined with the recommended rate of mineral fertilizer (cotton: 150 kg.ha-1 NPK , 50 kg.ha-1 Urea; maize:  200 kg.ha-1 NPK, 100 kg.ha-1 Urea). The addition of biochar during the composting process increased the total nitrogen, total magnesium and total  phosphorus contents of co-composted biochar by 48%, 64% and 68% respectively compared to compost. Although the biochar-based amendments  did not improve the physico-chemical parameters of the soil, two years after their application, a significant increase in cotton yield by 14% (biochar)  and 19% (co-composted biochar) compared to compost in the third year was observed. Biochar-based amendments can be suggested to producers  for medium-term crop yield improvement

[Accepted Manuscript] A call to strengthen the global strategy against schistosomiasis and soil-transmitted helminthiasis: the time is now.

In 2001, the World Health Assembly (WHA) passed the landmark WHA 54.19 resolution for global scale-up of mass administration of anthelmintic drugs for morbidity control of schistosomiasis and soil-transmitted helminthiasis, which affect more than 1·5 billion of the world's poorest people. Since then, more than a decade of research and experience has yielded crucial knowledge on the control and elimination of these helminthiases. However, the global strategy has remained largely unchanged since the original 2001 WHA resolution and associated WHO guidelines on preventive chemotherapy. In this Personal View, we highlight recent advances that, taken together, support a call to revise the global strategy and guidelines for preventive chemotherapy and complementary interventions against schistosomiasis and soil-transmitted helminthiasis. These advances include the development of guidance that is specific to goals of morbidity control and elimination of transmission. We quantify the result of forgoing this opportunity by computing the yearly disease burden, mortality, and lost economic productivity associated with maintaining the status quo. Without change, we estimate that the population of sub-Saharan Africa will probably lose 2·3 million disability-adjusted life-years and US$3·5 billion of economic productivity every year, which is comparable to recent acute epidemics, including the 2014 Ebola and 2015 Zika epidemics. We propose that the time is now to strengthen the global strategy to address the substantial disease burden of schistosomiasis and soil-transmitted helminthiasis

Accuracy of two circulating antigen tests for the diagnosis and surveillance of schistosoma mansoni infection in low-endemicity settings of Co boolean AND te d'Ivoire

In low-endemicity settings, current tools for the diagnosis and surveillance of schistosomiasis are often inaccurate in detecting true infection. We assessed the accuracy of an up-converting phosphor lateral flow circulating anodic antigen (UCP-LF CAA) test and a point-of-care circulating cathodic antigen (POC-CCA) urine cassette test for the diagnosis of Schistosoma mansoni. Our study was conducted in eight schools of western Cote d'Ivoire. Fifty children, aged 9-12 years, were enrolled per school. From each child, a single urine specimen and two stool specimens were collected over consecutive days for diagnostic work-up. Urine samples were subjected to UCP-LF CAA and POC-CCA tests. From each stool sample, triplicate Kato-Katz thick smears were examined. Overall, 378 children had complete data records. The prevalence of S. mansoni, as assessed by six Kato-Katz thick smears, was 4.0%. The UCP-LF CAA and POC-CCA tests revealed S. mansoni prevalence of 25.4% and 30.7%, respectively, when considering trace results as positive, and prevalence of 23.3% and 10.9% when considering trace results as negative. In the latter case, based on a composite "gold" standard, the sensitivity of UCP-LF CAA (80.7%) was considerably higher than that of POC-CCA (37.6%) and six Kato-Katz thick smears (13.8%). The negative predictive value of UCP-LF CAA, POC-CCA, and six Kato-Katz thick smears was 92.8%, 79.8%, and 74.1%, respectively. Our results confirm that UCP-LF CAA is more accurate than Kato-Katz and POC-CCA for the diagnosis of S. mansoni in low-endemicity settings.Cancer Signaling networks and Molecular Therapeutic

REPEATED DOSES OF PRAZIQUANTEL IN SCHISTOSOMIASIS TREATMENT (REPST) - SINGLE VERSUS MULTIPLE PRAZIQUANTEL TREATMENTS IN SCHOOL-AGE CHILDREN IN COTE D'IVOIRE: A STUDY PROTOCOL FOR AN OPEN-LABEL, RANDOMIZED CONTROLLED TRIAL

Host-parasite interactio

REPEATED DOSES OF PRAZIQUANTEL IN SCHISTOSOMIASIS TREATMENT (REPST) - SINGLE VERSUS MULTIPLE PRAZIQUANTEL TREATMENTS IN SCHOOL-AGE CHILDREN IN COTE D'IVOIRE: A STUDY PROTOCOL FOR AN OPEN-LABEL, RANDOMIZED CONTROLLED TRIAL

Host-parasite interactio

Limited efficacy of repeated praziquantel treatment in Schistosoma mansoni infections as revealed by highly accurate diagnostics, PCR and UCP-LF CAA (RePST trial)

BackgroundMost studies assessing praziquantel (PZQ) efficacy have used relatively insensitive diagnostic methods, thereby overestimating cure rate (CR) and intensity reduction rate (IRR). To determine accurately PZQ efficacy, we employed more sensitive DNA and circulating antigen detection methods.MethodologyA sub-analysis was performed based on a previously published trial conducted in children from Cote d'Ivoire with a confirmed Schistosoma mansoni infection, who were randomly assigned to a standard (single dose of PZQ) or intense treatment group (4 repeated doses of PZQ at 2-week intervals). CR and IRR were estimated based on PCR detecting DNA in a single stool sample and the up-converting particle lateral flow (UCP-LF) test detecting circulating anodic antigen (CAA) in a single urine sample, and compared with traditional KatoKatz (KK) and point-of-care circulating cathodic antigen (POC-CCA).Principal findingsIndividuals positive by all diagnostic methods (i.e., KK, POC-CCA, PCR, and UCP-LF CAA) at baseline were included in the statistical analysis (n = 125). PCR showed a CR of 45% (95% confidence interval (CI) 32-59%) in the standard and 78% (95% CI 66-87%) in the intense treatment group, which is lower compared to the KK results (64%, 95% CI 52-75%) and 88%, 95% CI 78-93%). UCP-LF CAA showed a significantly lower CR in both groups, 16% (95% CI 11-24%) and 18% (95% CI 12-26%), even lower than observed by POCCCA (31%, 95% CI 17-35% and 36%, 95% CI 26-47%). A substantial reduction in DNA and CAA-levels was observed after the first treatment, with no further decrease after additional treatment and no significant difference in IRR between treatment groups.Conclusion/SignificanceThe efficacy of (repeated) PZQ treatment was overestimated when using egg-based diagnostics (i.e. KK and PCR). Quantitative worm-based diagnostics (i.e. POC-CCA and UCPLF CAA) revealed that active Schistosoma infections are still present despite multiple treatments. These results stress the need for using accurate diagnostic tools to monitor different PZQ treatment strategies, in particular when moving toward elimination of schistosomiasis.Author summaryEfficacy of praziquantel (PZQ) for the treatment of schistosomiasis is usually assessed by classical microscopic detection of parasite eggs in stool or urine. Due to low sensitivity, especially in case of low-intensity infections, the prevalence of infection is underestimated leading to an overestimated cure rate (CR) when using these methods. In a repeated treatment trial, the efficacy of one versus four repeated PZQ treatments, given at 2-week intervals, was investigated in school-aged children from Cote d'Ivoire by applying a range of diagnostic methods, including traditional microscopy as well as more sensitive DNA and circulating antigen detection methods. Our results demonstrate that PZQ efficacy measurements vary based on the diagnostic method used: while egg-based diagnostics (stool microscopy and DNA detection methods) show an improved CR after repeated treatment, the CR determined by worm-based diagnostics (urine circulating antigen detection methods) remained poor over time. Although all four diagnostic methods showed a significant reduction in intensity of infection already after a single treatment, more accurate antigen diagnostics revealed that, in most cases, worms remain present even after multiple treatments. Hence, using accurate diagnostic tools is essential to determine the true infection status and to monitor and evaluate treatment programs