Lipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary cells in the presence of adenovirus

Endotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin fibroblasts, primary human melanoma cells) in the presence of entry-competent adenovirus particles. Toxicity can be observed with as little as 100 ng/ml free LPS or 100 pg/ml LPS when the LPS is assembled into polylysine/adenovirus complexes. Simple and effective methods of removing the contaminating LPS using either a polymyxin B resin or Triton X-114 extraction are described. Treatment of DNA samples to remove LPS eliminates the toxicity to primary cells

Could Stannern-trend eucrites be crustal-contaminated melts?

In this paper, we show that the composition of Stannern trend eucrites can be satisfactorily explained by contamination of normal main group eucrites by a crustal partial melt

Infectious diseases epidemic threats and mass gatherings: refocusing global attention on the continuing spread of the Middle East Respiratory syndrome coronavirus (MERS-CoV)

Media and World Health Organization (WHO) attention on Zika virus transmission at the 2016 Rio Olympic Games and the 2015 Ebola virus outbreak in West Africa diverted the attention of global public health authorities from other lethal infectious diseases with epidemic potential. Mass gatherings such as the annual Hajj pilgrimage hosted by Kingdom of Saudi Arabia attract huge crowds from all continents, creating high-risk conditions for the rapid global spread of infectious diseases. The highly lethal Middle Eastern respiratory syndrome coronavirus (MERS-CoV) remains in the WHO list of top emerging diseases likely to cause major epidemics. The 2015 MERS-CoV outbreak in South Korea, in which 184 MERS cases including 33 deaths occurred in 2 months, that was imported from the Middle East by a South Korean businessman was a wake-up call for the global community to refocus attention on MERS-CoV and other emerging and re-emerging infectious diseases with epidemic potential. The international donor community and Middle Eastern countries should make available resources for, and make a serious commitment to, taking forward a “One Health” global network for proactive surveillance, rapid detection, and prevention of MERS-CoV and other epidemic infectious diseases threats

The Diderot meteorite: The second chassignite

The Diderot meteorite is a dunite discovered in Sahara. The martian origin is unambiguous and Diderot shares strong petrographical similarities with Chassigny

Infectious diseases epidemic threats and mass gatherings: Refocusing global attention on the continuing spread of the Middle East Respiratory syndrome coronavirus (MERS-CoV)

Media and World Health Organization (WHO) attention on Zika virus transmission at the 2016 Rio Olympic Games and the 2015 Ebola virus outbreak in West Africa diverted the attention of global public health authorities from other lethal infectious diseases with epidemic potential. Mass gatherings such as the annual Hajj pilgrimage hosted by Kingdom of Saudi Arabia attract huge crowds from all continents, creating high-risk conditions for the rapid global spread of infectious diseases. The highly lethal Middle Eastern respiratory syndrome coronavirus (MERS-CoV) remains in the WHO list of top emerging diseases likely to cause major epidemics. The 2015 MERS-CoV outbreak in South Korea, in which 184 MERS cases including 33 deaths occurred in 2 months, that was imported from the Middle East by a South Korean businessman was a wake-up call for the global community to refocus attention on MERS-CoV and other emerging and re-emerging infectious diseases with epidemic potential. The international donor community and Middle Eastern countries should make available resources for, and make a serious commitment to, taking forward a "One Health" global network for proactive surveillance, rapid detection, and prevention of MERS-CoV and other epidemic infectious diseases threats

Examining Aspects of Digital Inclusion Among National Samples of US Older Adults

We live in a world where we are constantly connected to devices (e.g., smartphones, computers, tablets) and are encouraged to go online to find information about most things in society. This constant digital connection provides the means whereby many individuals communicate and exchange social support. For most demographic groups, this results in being online and connected to devices multiple times each day. Older adults have been slower to adopt and use emerging information and communication technologies (ICTs). Their digital divide in comparison to other age groups may not be an accurate representation of their technology use and the reasons for this use. This descriptive study examines this view of digital inclusion by focusing on older adults and their uses of technology. We provide an overview of technology usage by different older adult age groups in the United States using existing national‐level data. We utilize life course and aging theoretical perspectives to help articulate how older adults use a wide variety of ICTs and whether they are constantly connected, and we note that while a constant connection to devices may be normative for younger age groups, this may not, and perhaps should not, be the case for older adults. The article concludes with a discussion of the social construction of digital inclusion and emphasizes the significant variation that exists in this construct, measurement of technology use in large‐scale datasets, and variation in technology use across older adult life course groups