Effects of bacterial lipopolysaccharide exposure on immune responsiveness in a rodent model of Parkinson’s disease

The effects of lipopolysaccharide (LPS) on immune modulation in rats subjected to a right-unilateral lesion of the substantia nigra neurons by means of 6-hydroxydopamine (6-OHDA), were investigated. LPS administration (250 μg) significantly decreases the total number of leukocytes and erythrocytes, as well as the hemoglobin level in the 6-OHDAlesioned rats. In addition, LPS administration was also associated with an increase, relative to control, in the erythrocyte indexes and the phagocytosis by neutrophils, and in blastic transformation of T lymphocytes. The obtained data indicated that LPS exposure might represent a risk factor for the development of the immunological changes associated with Parkinson’s disease

Hematological changes in multiple myeloma

This paper presents the results of some hematological investigations which were done in the laboratory of the Municipal Emergency Hospital, Barlad on a number of 10 patients diagnosed with multiple myeloma. This type of blood cancer, also called plasmocytoma or Kahler’s disease, is characterized by a malignant proliferation of the plasmocytes in the bone marrow with serious hematopoietic perturbations, dramatic increase of ESR, bones pain, hypercalcemia, renal affection, etc. Our results confirm both the grouping of the red cells into scrolls and the presence of the big myeloma plasmocytes, grouped into nests ( foci), and sometimes can take unusual shapes (dumb bells). The hemoglobin and hematocryte decrease untill 63-65% compared to the normal physiological limit, the number of red cells reaches up to 20-23% from the normal limit, both for men and women, and the ESR amplifies compared to the normal as it follows: up to 12 times for men ( 65-158 mm/h) and up to 13,2 times for women ( 33-156 mm/h). The white cells and thrombocytes present some restricted numerical changes compared to the red series

CHARACTERIZATION OF ANEMIA IN MULTIPLE MYELOMA WITH THE HELP OF ERYTHROCYTE CONSTANTS

This paper intends to shed extra light on different types of anemia as caused by multiple myeloma in patients registered at “ Elena Beldiman” Municipal Emergency Hospital from Barlad, between 2001-1012. In order to complete this characterization, the values of derived erythrocyte constants have been used: the average volume of erythrocytes (VEM); mean erythrocyte hemoglobin (HEM) and the average concentration of erythrocyte hemoglobin (CHEM). Moreover, this paper aims to compare the different types of anemia in patients with multiple myeloma, according to their gender and age. The 34 identified patients were divided into four study groups, men and women aged under 60, respectively over 60 years.         It has been noticed that the incidence of macrocytic anemia (increased VEM) coexists with hypochromia (decreased CHEM), and the values from the four study groups are comparable, both types of anemia having mollified levels of severity, both in male patients and female patients

Blood glucose, serum proteins levels and superoxide dismutase activity in Cyprinus carpio, Hypophthalmichthys molitrix and Aristichthys nobilis following thermal stress

The study develops a comparative analysis of the mean levels of blood glucose and of total serum proteins, as well as of the activity of superoxide dismutase in one summer-old common carp (Common carp), silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis), both prior to and following the wild wintering conditions of the 2006 - 2007 season. Such parameters represent important biochemical markers in stress evaluation, once known that a constantly increasing stress results in higher values of glycemy and in superoxide dismutase activity, although decreasing proteinemy. In the one summer-old fry taken into study, significantly higher post-hibernal values of glycemy - with 84.9% in bighead carp, 139.1% in common carp and 198.1% in silver carp -were recorded. Thermal stress reduces proteinemy with 11% in bighead carp and with 14% in silver carp while, in the case of common carp, spring viremia reduces proteinemy with 13%, comparatively with the mean pre-hibernal values

Correlations between the proteinemy and glycemy of some cyprinidsand the antiparasitary treatments applied

The paper analyzes the modifications produced in some biochemical indices (proteinemy and glycemy), determined in the blood of certain one year-old culture cyprinids, namely: common carp (Cyprinus carpio), silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis), subjected to some prophylactic antiparasitary treatments. The experiment was performed between April 2007 and April 2008, in 0.5 ha ponds, each basin being populated with 79% common carp (245 g/piece), 11% silver carp (475 g/piece) and 10% bighead carp (425 g/piece). In the reference pond, no treatments were applied, while the experimental variant was prophylactically treated both in the moment of ponds filling (April 2007) and during the growing period, with trichlorfon, applied in preventive doses of 0.1 mg/L, in two steps, and calcium chloride, 2 kg/ha, twice a week, respectively. The concentration values of the biochemical indices were determined one year after the experiment (March-April 2008). The results obtained attest that the preventive anti-ectoparasitary treatment applied to the three fish species has positive effects on their physiological condition, generally, on proteinemy and glycemy - especially. In the treated silver carp, spring proteinemy is 23% lower, while glycemy is 35% lower - comparatively with the reference. In the treated common carp, the two biochemical indices show an increasing tendency, with 19% in proteinemy and 27% in glycemy - respectively

Influence of the antiectoparasitary treatment on cellular respiration of some cultured cyprinid species

: The study discusses the influence of a preventive treatment against ectoparasites on the intensity of cellular respiration in three, 3 summer-old cultured cyprinid species, namely: common carp (Cyprinus carpio), silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis). The cellular consumption of oxygen (μLO2/g fresh tissue) in muscles and gills was determined by the Warburg microrespirometric method, on two batches of fish from each species, namely: a reference (untreated) batch and a batch treated against ectoparasites. The results obtained show that the intensity of the cellular consumption of oxygen differs - as a function of the treatment applied, fish species, cellular type and duration of recordings. Therefore, generally speaking, cellular respiration is more intense in gills than in muscles, in both types of batches: treated and untreated. Lower values are registered in common carp, followed, in increasing order, by those recorded in silver carp and bighead carp. The antiectoparasitary treatment has positive effects, intensifying cellular respiration, along with metabolism stimulation and an improved general physiological condition of fi

COMPARATIVE INVESTIGATIONS ON THE ACTIVITY OF SOME INTESTINAL AMINOTRANSFERASES, IN THE ARISTICHTHYS NOBILIS SPECIES, IN VARIOUS STAGES OF DEVELOPMENT

The paper is devoted, on one side, to a comparative study of the length of the digestive tube versus both the total and the standard length of the body, on the other, to the activity of aspartate- and alanine-aminotransferasic activity manifested in the median segment of the digestive tube, in one, and, respectively, four summer-old individuals of Aristichthys nobili

Hematological profile variation in the european bison (Bison bonasus l., 1758) as a function of age, sex and health condition

The paper analyzes some new hematological data on the European bison from the Natural Park of Vanatori Neamt, a comparison being made both between the values recorded in animals of different ages (2-8 years) and sexes, and between the values of the European bison and those registered in some kindred ruminant mammals, such as the deer and the cow. More than that, a comparison is made between the data taken over from healthy bisons and from an animal affected by severe anemia and multiple parasitary indices. Hematological prosperity increases with the age in female bisons, while a decrease is reported in males, especially in the case of suffering animals, versus the healthy ones. Comparatively with other big mammals, the hematological profile of the bison is much more similar to that of domestic ruminants, being wholly different from that of the wild deer

Modification of some biochemical parameters and of the concentration of some serum ions in Cyprinus carpio L. species grown under different sanitary-veterinary conditions

The paper discusses the modifications produced in some biochemical indices as well as in the concentration of serum ions, in fry carp, during the period of active feeding, in two experimental ponds of the Iasi district, providing different conditions of ichtyo-pathological prevention. The average values of glucose and alkaline phosphatase from the blood, as well as those of cholesterol, ureic nitrogen, Na+, Ca2+ and K+ from the serum, have been determined after 120 days of experiment (June - September), for 11 representatives of each basin. For the two experimental variants, different preventive treatments - based on calcium chloride administered in three different moments of the growing cycle and on concentrated, drug-containing food for assuring antimicrobial prevention - have been applied. The values recorded in the variant under analysis are lower than those of the reference, with 12.2% for glycemy, 9.7% for total cholesterol, 17.5% for the total ureic nitrogen and 10.98%, respectively, for alkaline phosphatase. The levels of the determined ions show higher values in the experimental variant, with 23.3% for K+ and 4.1%, respectively, for Ca2+, while the average values for Na+ are lower in the fish from variant B, the Na+/K+, Na+/Ca2+ and (Na++K+)/Ca2+ ratios showing inferior values - with 4 - 21% - than the reference ones

THE EFFECTS OF SOME PESTICIDES ON THE CARDIAC ACTIVITY IN FROG (Rana ridibunda Pall.)

The paper analyses the experimental results on the effects of various doses of Oleokalus insecticide (0.15, 0.75and 1.5 mg%), Oltisan herbicid (0.25, 1 and 2 mg%) and Tilt fungicide (0.25, 1 and 3 mg%) upon the isolated heart offrog (Rana ridibunda Pall.) perfused with a Ringer physiological solution. The doses, similar to those applied inagricultural practice, are considered as non-toxic for the environment (Baicu, 1979). The mechano-cardiogramsperformed showed that all pesticides under analysis cause depression in the cardiac activity of frog, its intensity andpersistence depending on the substance and dose applied. The maximum remanent cardio-inhibiting effects are inducedby fungicide Tilt