31 research outputs found
Consequences of negative energy balance on follicular development and oocyte quality in primiparous sows
Metabolic demands of modern hybrid sows have increased over the years, which increases the chance that sows enter a substantial negative energy balance (NEB) during lactation. This NEB can influence the development of follicles and oocytes that will give rise to the next litter. To study effects of a lactational NEB on follicular development, we used 36 primiparous sows of which 18 were subjected to feed restriction (3.25 kg/day) and 18 were full-fed (6.5 kg/day) during the last two weeks of a 24.1 ± 0.3 day lactation. Feed restriction resulted in a 70% larger lactational body weight loss and 76% higher longissimus dorsi depth loss, but similar amounts of backfat loss compared to the full fed sows. These changes were accompanied by lower plasma insulin-like growth factor 1 (IGF1) and higher plasma creatinine levels in the restricted sows from the last week of lactation onwards. Ovaries were collected 48 h after weaning. Restricted sows had a lower average size of the 15 largest follicles (−26%) and cumulus-oocyte complexes showed less expansion after 22 h in vitro maturation (−26%). Less zygotes of restricted sows reached the metaphase stage 24 h after in vitro fertilization and showed a higher incidence of polyspermy (+89%). This shows that feed restriction had severe consequences on oocyte developmental competence. Follicular fluid of restricted sows had lower IGF1 (−56%) and steroids levels (e.g. β-estradiol, progestins and androgens), which indicated that follicles of restricted sows were less competent to produce steroids and growth factors needed for oocytes to obtain full developmental competence
Local Environmental Knowledge of School Students
Place, it is acknowledged by many, is a social construction, imbued with meaning and located in both time and space by the people that interact with that space (Vanclay, 2008, p. 17). Our understanding of this and how people create a meaningful world and understand their place has been a central theme in geography typified by the foundational work of Yi-Fu Tuan (1974, 1977) and Edward Relph (1976). © 2015 Sense Publishers. All rights reserved