Isolamento de proteínas Cyt de Bacillus thuringiensis subsp. israelensis por gradiente de sacarose.

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L’utilisation combinée des scores GRACE et CRUSADE pour la stratification du risque d’infarctus du myocarde

BACKGROUND: Risk assessment is fundamental in the management of acute coronary syndromes (ACS), enabling estimation of prognosis. AIMS: To evaluate whether the combined use of GRACE and CRUSADE risk stratification schemes in patients with myocardial infarction outperforms each of the scores individually in terms of mortality and haemorrhagic risk prediction. METHODS: Observational retrospective single-centre cohort study including 566 consecutive patients admitted for non-ST-segment elevation myocardial infarction. The CRUSADE model increased GRACE discriminatory performance in predicting all-cause mortality, ascertained by Cox regression, demonstrating CRUSADE independent and additive predictive value, which was sustained throughout follow-up. The cohort was divided into four different subgroups: G1 (GRACE<141; CRUSADE<41); G2 (GRACE<141; CRUSADE≥41); G3 (GRACE≥141; CRUSADE<41); G4 (GRACE≥141; CRUSADE≥41). RESULTS: Outcomes and variables estimating clinical severity, such as admission Killip-Kimbal class and left ventricular systolic dysfunction, deteriorated progressively throughout the subgroups (G1 to G4). Survival analysis differentiated three risk strata (G1, lowest risk; G2 and G3, intermediate risk; G4, highest risk). The GRACE+CRUSADE model revealed higher prognostic performance (area under the curve [AUC] 0.76) than GRACE alone (AUC 0.70) for mortality prediction, further confirmed by the integrated discrimination improvement index. Moreover, GRACE+CRUSADE combined risk assessment seemed to be valuable in delineating bleeding risk in this setting, identifying G4 as a very high-risk subgroup (hazard ratio 3.5; P<0.001). CONCLUSIONS: Combined risk stratification with GRACE and CRUSADE scores can improve the individual discriminatory power of GRACE and CRUSADE models in the prediction of all-cause mortality and bleeding. This combined assessment is a practical approach that is potentially advantageous in treatment decision-making

Building with sugar and corn

Two alternative and highly sustainable building techniques are presented and described. The techniques are sugar reinforcement of earth based material and corncob based thermal isolation material. Some experimental results highlight the advantages and disadvantages of these two proposed techniques whose study is still beginning. The water resistance of earth based materials may be increased by adding a certain amount of sugar. On the other hand, corn cob material may have a similar thermal behavior as the common thermal insulation materials currently used in the building industry

Comparação entre variedades de mangueira em relação a características do fruto nas condições do Submédio do São Francisco.

Este trabalho objetivou avaliar atributos do fruto de 14 variedades nas condições do Submédio do Vale do São Francisc

Castanha na roça: sinergia entre agricultura e extrativismo em uma unidade de conservação de uso sustentável - RESEX Cajari.

Este trabalho busca evidenciar a sinergia entre agricultura e extrativismo, demonstrando que existe um potencial produtivo de castanha da amazônia em áreas de roças e capoeiras provenientes da agricultura itinerante, que podem ser conduzidas como sistemas agroflorestais (SAFs). A partir do levantamento realizado em 14 comunidades da RESEX, foram inventariadas 90 áreas provenientes da agricultura com diferentes períodos de pousio. Dessas, 42 são capoeiras "abandonadas" e 48 são áreas que foram selecionadas, em 2012, para a agricultura. Nessas áreas foram encontradas 1.445 castanheiras (cast), a maior parte jovens, gerando uma densidade aproximada de 13 cast ha-1. A densidade nas capoeiras abandonadas foi de 17 cast ha-1 e nas capoeiras em cultivo foi de 8 cast ha1, confirmando que os castanheiros optam por colocar a roça em áreas com menor regeneração de castanheiras. Do total de castanheiras inventariadas, 7,4% são comprovadamente produtivas e muitas ainda não produzem frutos, mas já apresentam floração. Em algumas dessas áreas de capoeiras, os castanheiros já fazem a coleta dos ouriços. A interação, bem como a troca de conhecimento com os agroextrativistas, proporcionou uma compreensão melhor da realidade local e facilitou a realização do trabalho. A maior densidade de regenerações de castanheiras e maior crescimento nas áreas usadas para agricultura itinerante, do que dentro na floresta madura, justifica o manejo dessas áreas como se fossem SAFs, com a manutenção das castanheiras jovens no meio do roçado para formação de futuros castanhais

New extractants for separation of platinium-group metals from chloride solutions and their application to recycling processes

In this paper some results on the application of four N,N’-tetrasubstituted malonamide derivatives for the extraction and separation of platinum-group metals (PGMs) are presented. The above mentioned extractants were specifically synthesized in order to evaluate their efficiency for the extraction of a specific metal, or group of metals, from aqueous chloride matrices. Different behaviours were obtained towards platinum(IV) and/or palladium(II) extraction, all the N,N’-tetrasubstituted malonamide derivatives allowing the mutual separation of the two metal ions, depending on the HCl concentration. Generally, extraction experiments were carried out involving initial aqueous phases containing 100 mg/L of each metal isolate, in varying 1M to 8M HCl concentration ranges, and 0.05M extractant in 1,2- dichloroethane solutions. From the preliminary results obtained, one can conclude that HCl concentrations and the structure of the malonamide derivative play a crucial role on the efficiency shown for Pt(IV) and/or Pd(II) extraction. The efficiency of the stripping stage of the loaded organic phases is variable, depending on the N,N’-tetrasubstituted malonamide derivative, as well as on the metal ion involved; for Pt(IV), distilled water and 1M HCl were generally successful, whereas for Pd(II), thiourea in HCl gave better results. The overall data already achieved suggest that these extractants may be good candidates for further evaluation in PGMs separations from real solutions resulting from recycling processes

Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda.

Bacillus thuringiensis (Bt) endotoxins (Cyt and Cry) have been extensively explored for biological control of fall armyworm, Spodoptera frugiperda, an important pest in major corn-producing countries of America. Five hundred Bt isolates with different toxicities against S. frugiperda were characterized by PCR for the presence of cyt genes (cyt1, cyt2, cyt1Aa, cyt1Ab, cyt2B and cyt2Ba), and the effect of insecticidal proteins Cry1Ba, Cry1Ca, Cry1Da and Cyt on S. frugiperda larvae were evaluated. Six isolates showed the presence of cyt genes, three isolates harbored two gene families (cyt1 and cyt2), and three isolates harbored only one of the cyt gene families (cyt1 and cyt2). It was not possible to correlate the presence/absence of cyt genes with toxicity against S. frugiperda. In this study, cyt genes were present in toxic and nontoxic isolates to this insect pest. Bioassays against S. frugiperda larvae showed that only Cry1Ca protein had toxicity, with 77.08% of mortality. Synergism among Cry and Cry proteins used in this study against S. frugiperda was not observed

Programa de melhoramento genético da raça Girolando - teste de progênie: sumário de touros 2008.

bitstream/item/89920/1/Doc-126-Girolando.pd

Decrease in Bone Mass in Women After Liver Transplantation: Associated Factors

AbstractBackgroundIn the future, an increasing number of female liver transplant recipients will reach the climacteric with osteoporosis as a common complication. We evaluated the factors associated with decreased bone mass among women after liver transplantation.MethodsA prospective, cross-sectional study of 23 outpatient transplant recipients followed from February 2009 to March 2010 included women of age ≥35 years after liver transplantations ≥1 year prior. We recorded patient histories, liver enzyme levels, as well as bone mineral densities measured at the lumbar spine and femur. Statistical analysis used Fisher's exact test, simple odds ratio (OR), and Spearman's rank correlation coefficient.ResultsThe mean patient age was 52.5 ± 11 years with 30.4% premenopausal, and 69.6% perimenopausal or postmenopausal. Approximately 21% showed osteoporosis and 35%, a low bone mass. Postmenopausal women: OR 69.0 (95% CI 2.89–1647.18; P < .0001), aged ≥49 years: OR 13.33 (95% CI 1.78–100.15; P = .0123) and receiving a transplant after 44 years of age: OR 49.50 (95% CI 3.84–638.43; P < .0001) were associated with a lower bone mass. Having undergone transplantation for more than 5.8 years lowered the risk of bone mass change: OR 0.11 (95% CI 0.02–0.78; P = .0361). Clinical and laboratory variables, including corticosteroid use, were not associated with decreased bone mass.ConclusionUnderstanding the prevalence and factors associated with osteoporosis among female liver transplant recipients is important to enhance the strategies to diagnose and treat these women, seeking to improve their quality of life