Activation of locus coeruleus heme oxygenase-carbon monoxide pathway promoted an anxiolytic-like effect in rats

The heme oxygenase-carbon monoxide pathway has been shown to play an important role in many physiological processes and is capable of altering nociception modulation in the nervous system by stimulating soluble guanylate cyclase (sGC). In the central nervous system, the locus coeruleus (LC) is known to be a region that expresses the heme oxygenase enzyme (HO), which catalyzes the metabolism of heme to carbon monoxide (CO). Additionally, several lines of evidence have suggested that the LC can be involved in the modulation of emotional states such as fear and anxiety. The purpose of this investigation was to evaluate the activation of the heme oxygenase-carbon monoxide pathway in the LC in the modulation of anxiety by using the elevated plus maze test (EPM) and light-dark box test (LDB) in rats. Experiments were performed on adult male Wistar rats weighing 250-300 g (n=182). The results showed that the intra-LC microinjection of heme-lysinate (600 nmol), a substrate for the enzyme HO, increased the number of entries into the open arms and the percentage of time spent in open arms in the elevated plus maze test, indicating a decrease in anxiety. Additionally, in the LDB test, intra-LC administration of heme-lysinate promoted an increase on time spent in the light compartment of the box. The intracerebroventricular microinjection of guanylate cyclase, an sGC inhibitor followed by the intra-LC microinjection of the heme-lysinate blocked the anxiolytic-like reaction on the EPM test and LDB test. It can therefore be concluded that CO in the LC produced by the HO pathway and acting via cGMP plays an anxiolytic-like role in the LC of rats

Acute stress-induced antinociception is cGMP-dependent but heme oxygenase-independent

Endogenous carbon monoxide (CO), which is produced by the enzyme heme oxygenase (HO), participates as a neuromodulator in physiological processes such as thermoregulation and nociception by stimulating the formation of 3′,5′-cyclic guanosine monophosphate (cGMP). In particular, the acute physical restraint-induced fever of rats can be blocked by inhibiting the enzyme HO. A previous study reported that the HO-CO-cGMP pathway plays a key phasic antinociceptive role in modulating noninflammatory acute pain. Thus, this study evaluated the involvement of the HO-CO-cGMP pathway in antinociception induced by acute stress in male Wistar rats (250-300 g; n=8/group) using the analgesia index (AI) in the tail flick test. The results showed that antinociception induced by acute stress was not dependent on the HO-CO-cGMP pathway, as neither treatment with the HO inhibitor ZnDBPG nor heme-lysinate altered the AI. However, antinociception was dependent on cGMP activity because pretreatment with the guanylate cyclase inhibitor 1H-[1,2,4] oxadiazolo [4,3-a] quinoxaline-1-one (ODQ) blocked the increase in the AI induced by acute stress

Evolution of the thyroid hormone-binding protein, transthyretin

Transthyretin (TTR) belongs to a group of proteins, which includes thyroxine-binding globulin and albumin, that bind to and transport thyroid hormones in the blood. TTR is also indirectly implicated in the carriage of vitamin A through the mediation of retinol-binding protein (RBP). It was first identified in 1942 in human serum and cerebrospinal fluid and was formerly called prealbumin for its ability to migrate faster than serum albumin on electrophoresis of whole plasma

Variações florística e estrutural e relações fitogeográficas de um fragmento de floresta decídua no Rio Grande do Norte, Brasil Floristic and structural variations, and the phytogeographical relationships of a deciduous forest fragment in Rio Grande do Norte State, Brazil

O trabalho foi realizado num fragmento de floresta decídua com cerca de 270ha centrado em 5°53'S e 35°23'W e que se estende por dois ambientes edáficos distintos (Moda 1 e Moda 2). Os objetivos do trabalho foram caracterizar florística e estruturalmente o componente arbóreo nos dois ambientes, compará-los entre si e avaliar suas relações fitogeográficas. Todos os indivíduos vivos e mortos em pé com perímetro à altura do peito >10cm foram considerados como árvore e amostrados pelo método dos quadrantes. Para Moda 1 e Moda 2 foram estimados, respectivamente: densidade total de 1.587 e de 1.924 indivíduos.ha-1, área basal total de 15,88 e de 15,86m².ha-¹, freqüências modais das alturas entre 5 e 5,9m e entre 6 e 6,9m e dos diâmetros de caule entre 5,0 e 9,9cm e entre 3,2 e 4,9cm, índice de diversidade de Shannon de 3,19 e de 3,26 e índice de eqüabilidade de Pielou de 0,79 e de 0,86. Os estratos arbóreos nos dois ambientes foram considerados estruturalmente semelhantes. Foram observadas no total 66 espécies de árvores, sendo 56 em Moda 1 e 45 em Moda 2. Piptadenia moniliformis Benth. é a espécie com maior valor de importância nas duas áreas, seguidas por Tabebuia impetiginosa (Mart. ex DC.) Standl. em Moda 1 e por Chamaecrista ensiformis (Vell.) H.S. Irwin & Barneby em Moda 2. As áreas apresentam grande similaridade florística (S S = 0,69 e S C = 0,53), sendo que as espécies características de matas decíduas são mais abundantes em ou exclusivas de Moda 1, e as espécies características das Caatingas, em Moda 2. As pequenas diferenças florística e estrutural entre os dois setores da mata podem ser atribuídas às condições edáficas. Em termos florísticos, fisionômicos e ambientais, a mata foi classificada como um tipo de vegetação de transição entre as províncias fitogeográficas Atlântica e das Caatingas, com a presença destacada de espécies com ampla distribuição nas matas secas neotropicais e nos Cerrados.<br>This work was undertaken in a deciduous forest fragment (~270 ha, centered on 5°53'S and 35°23'W) spanning two distinct edaphic environments (Areas 1and 2), and sought to floristically and structurally characterize the tree layers in both environments, as well as to assess their phytogeographical relationships. All living and standing dead trees with CBH >10 cm were sampled using the point-centered quarter method. The results for areas 1and 2 were, respectively: total density, 1.587 and 1.924 individual.ha-1; total basal area, 15.88 and 15.86m².ha-1; most frequent height classes of living trees, 5-5.9m and 6-6.9m; most frequent stem diameters of living trees, 5.09.9cm and 3.2-4.9cm; Shannon diversity index 3.19 and 3.26; and Pielou evenness index, 0.79 and 0.86. The tree layers in both areas 1 and 2 were considered structurally similar. A total of 66 tree species were observed (56 in area 1 and 45 in area 2). Piptadenia moniliformis Benth. showed the greatest importance value in both areas, followed by Tabebuia impetiginosa (Mart. ex DC.) Standl. in area 1, and Chamaecrista ensiformis (Vell.) H.S. Irwin & Barneby in area 2. Both edaphic environments showed a high floristic similarity (S S = 0.69 and S C = 0.53).Species typical of deciduous forests were more abundant in, or exclusive to, area 1. Species more typical of Caatingas environments were more abundant in, or exclusive to, area 2. The small floristic and structural differences noted between the two areas are apparently attributable to soil conditions. This forest fragment contains many species showing wide distributions in both Neotropical dry forests and Cerrados, and was considered a transitional vegetation type between the Atlantic and Caatingas phytogeographical provinces, in terms of species composition and physiognomy, as well as local environment conditions