Repurposing of Meropenem and Nadifloxacin for Treatment of Burn Patients?

The escalating number of multidrug resistant pathogens has demanded the swift development of new and potent antibiotics (ref. 2). Metallo-[beta]-lactamases (MBLs) continue to evolve, rendering the latest generation of carbapenem antibiotics useless (ref. 8). SPM-1, a recently discovered MBL, was isolated from a juvenile leukemia patient residing in a hospital in San Palo, Brazil just prior to the patient succumbing to septicemia brought on by Pseudomonas aeruginosa expressing SPM-1 (ref. 8). Screening of the Johns Hopkins Compound library of 1,514 FDA or FAD approved drugs (ref. 1) identified a novel SPM-1 inhibitor that is synergistically compatible with meropenem. Using clinically achievable concentrations, meropenem coupled with nadifloxacin inhibits Pseudomonas aeruginosa expressing SPM-1. This shotgun approach to new drug discovery provided a prompt solution to the grave problem of antibiotic resistant pathogens that are thriving in hospitals today

Hexagon Mechanized Ball Bar Fixture

Team nineteen was partnered with Hexagon Manufacturing Intelligence and tasked with the assignment of automating a ball bar fixture used in error mapping of coordinate measurement machines. This design report outlines the processes employed during the design by the team, with the long term goal of creating a functional prototype of the product. This semester work was done in conceptual design and analysis to meet the design specifications developed by Hexagon and Team 19. The culmination of this semesters work is presented in a Proof of concept, with the intent of fabrication and manufacture, along with redesign and optimization being completed in the coming semester. Error mapping is a process employed in the calibration of CMMs. This process is done by placing a Bar Ball in 12 different positions within the measurable volume of the CMM and taking a measurements on the surface of each Ball. The center of each ball is calculated along with the length distance between centers. Analysis is done of the change in measured length of the Bar Ball in each position and an error map is created to offset the known errors. By utilizing this process CMMs can be accurate to less one micron. The problem faced by Hexagon is that currently this error mapping process must be done by hand which leads to a very labor intensive process. The Bar Ball must be moved into placed, then measurements taken manually with the CMM. Hexagon has charged Team 19 with creating a solution to this problem by automating the process. The goal of this project is to create a machine that can placed within the CMM, and after initial set up, can carry out the entire error mapping process without any human interaction. The design solution proposed by Team 19 is a four degree of freedom robotic manipulator. Three linkages with rotation at both the base of the machine and the center of the affixed Bar Ball provide access to all 12 of the required positions. The machine will utilize worm gear driven, DC motors in order to actuate each DOF of the manipulator, and will be controlled with an Arduino Mega AVR development microcontroller. The control software will be integrated into the system and work in tandem with the CMM control software PC-DMIS in order to completely automate the placement and measurement of the Bar Ball in the error mapping process

Impact of Observed and Controlled Water Intake on Reticulorumen Temperature in Lactating Dairy Cattle

Dairy precision technologies helps producers monitor individual animals. Reticulorumen temperature boluses are a way to monitor core body temperature; however, factors such as water intake affects reticulorumen temperature. This research determined the effect of natural water intake and a controlled water drench on reticulorumen temperature (RT) in dairy cattle. In observational study part 1, tie- stall cows (n = 4) with RT transponders were observed for natural water intake (recorded by in line water meters) for 48 h. In experiment part 2, a randomized Latin square design with cows (n = 12) restricted on feed for 4 h, were drenched daily with a water quantity of 6.7 L, 11.4 L or 22.7 L, and at controlled water temperature of 1.7 °C, 7.2 °C, 15.5 °C, or 29.4 °C. Descriptively, observational study 1 had (Mean ± SD 0.27 ± 0.31 L ingested per drinking event (n = 84) and RT decline from baseline was 2.29 ± 1.82 °C. For the experiment, a 48-h specific rolling baseline temperature range (BTR) was calculated for each cow prior to the experiment to determine time required for RT to reach BTR, and time to return to BTR. In part 2 of the experiment, as water quantity increased, RT had a greater maximum degree drop from baseline. Water temperature and water quantity interaction influenced time required for BTR to reestablish. The coldest water temperature at the highest drench quantity affected time for BTR to reestablish the longest (103 min). Results from this study suggest that an algorithm could be designed to predict water intake events for producers using reticulorumen temperature

Excess mortality among 10‐year survivors of classical Hodgkin lymphoma in adolescents and young adults

Adolescents and young adults (AYA) surviving classical Hodgkin lymphoma (cHL) risk long term fatal treatment‐related toxicities. We utilized the Surveillance, Epidemiology and End Results (SEER) program to compare excess mortality rate (EMR‐observed minus expected mortality) for 10‐year survivors of AYA cHL diagnosed in 1973–1992 and 1993–2003 eras. The 15‐year EMR reduced from 4.88% to 2.19% while the 20‐year EMR reduced from 9.46% to 4.07% between eras. Survivors of stages 1–2 had lower EMR than survivors of stages 3–4 cHL in the 1993‐2003 but not in the 1973–1992 era. There was an overall decline in risk of death between 10 and 15 years from diagnosis, driven mostly by second neoplasms and cardiovascular mortality. Despite reduction in fatal second neoplasms and cardiovascular disease with more current therapy, long term survivors of AYA cHL still have a higher risk of death than the general population highlighting the need for safer therapies.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/142133/1/ajh24964_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/142133/2/ajh24964.pd