2 research outputs found
Application of conventional and real-time fluorescent ITS1 rDNA PCR for detection of Besnoitia besnoiti infections in bovine skin biopsies
Besnoitia besnoiti, an apicomplexan protozoan parasite, is the causative agent of bovine besnoitiosis. This infection may
dramatically affect body condition, and, in males, lead to irreversible infertility. While identification of clinical cases and their
histopathological confirmation is relatively simple to carry out, finding subclinical forms of infection is more difficult, thus a more
sensitive test for the identification of the etiological agent may be an appropriate diagnostic tool. We have developed the ITS1
rDNA-sequence-based conventional and real-time PCR which are highly sensitive and specific for the detection of B. besnoiti
infection in cattle. A recombinant internal positive control was introduced to assess possible sample-related inhibitory effects
during the amplification reaction and, in order to prevent false-positive results, a pre-PCR treatment of potentially contaminating
dU-containing PCR product with uracyl-DNA-glycosylase (UDG) was followed.
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