Características espeleológicas del karst de la región de Gibara

[spa] La región de Gibara, una de las más interesantes del país, no tenía hasta el presente un estudio que ofreciera una visión general de las características de las cavernas que posee. Sólo se habían realizado trabajos puntuales en las distintas cuevas y simas conocidas, los cuales no ofrecían datos acerca del karst en conjunto de la zona. Para realizar este trabajo los autores tuvieron en cuenta toda la información de los distintos grupos espeleológicos e investigadores que han estudiado, de una forma u otra, la región. Posteriormente se analizó toda la información que sobre las cavernas se poseía, la cual fue procesada en valores porcentuales para poder brindar, de una manera más explícita, un panorama de las características espeleológicas de esta interesante región del oriente cubano.[eng] The Región de Gibara, one of Cuba's most interesting, has not been previously studied with the aim of producing an overall view of its karst and caves; only specific studies have been carried out on its known caves and potholes, and these do not give us a general view of this region's karst. In order to carry out this task, the authors have taken into account the work produced by different speleological groups and investigators who have studied, in one way or another, this region. The collected data was then analysed and represented in percentages to afford a more informative way of viewing the speleological characteristics of this eastern Cuban region

Ontogenesis of Gonadotropin-Releasing Hormone Neurons: A Model for Hypothalamic Neuroendocrine Cell Development

The vertebrate hypothalamo–pituitary–gonadal axis is the anatomical framework responsible for reproductive competence and species propagation. Essential to the coordinated actions of this three-tiered biological system is the fact that the regulatory inputs ultimately converge on the gonadotropin-releasing hormone (GnRH) neuronal system, which in rodents primarily resides in the preoptic/hypothalamic region. In this short review we will focus on: (1) the general embryonic temporal and spatial development of the rodent GnRH neuronal system, (2) the origin(s) of GnRH neurons, and (3) which transcription – and growth factors have been found to be critical for GnRH neuronal ontogenesis and cellular fate-specification. Moreover, we ask the question whether the molecular and cellular mechanisms involved in GnRH neuronal development may also play a role in the development of other hypophyseal secreting neuroendocrine cells in the hypothalamus

Longitudinal study of physical activity in college students: Testing self-determination theory based on stages of change

The main purpose of this study was to longitudinally test the relationships of Self Determination Theory variables on physical activity, analyzing the model based on stages of change. The study is a longitudinal and quantitative research. We have developed a longitudinal cross-lagged design for structural equation models of three time points and multi-group analysis. Participants were 772 Spanish college students from the University of Zaragoza (M = 19.74, SD = 2.76) studying several degrees. Participants completed different self-reports, assessing: physical activity, stages of change, motivation and basic psychological needs, at three time points (November, January and March). The analysis indicated that intrinsic motivation positively predicted physical activity at the 3-time points, both directly and indirectly. Intrinsic motivation was negatively predicted by autonomy and positively predicted by competence (from time point 2 to 3). No relationships were found with relatedness for the entire sample. Regarding the stages of change multi-group analysis were run: in the low active group, we found a negative relationship between relatedness and physical activity, and a positive association between competence and intrinsic motivation and physical activity, whereas intrinsic motivation did not predict physical activity. Our findings showed that intrinsic motivation was the most consistent predictor of physical activity. The competence need played an essential role in intrinsic motivation and physical activity for both active and passive subjects. However, the basic psychological needs have not worked as indicated by the theory when it was examined longitudinally. The findings of the study highlight the need for different strategies to improve physical activity levels according to the stages of change

Fractioning of Proanthocyanidins of Uncaria tomentosa. Composition and Structure-Bioactivity Relationship

In a previous study, the detailed low-molecular weight polyphenolic profile of the different plant parts (leaves, stem, bark and wood) of Uncaria tomentosa was reported, the leaves being the plant part with the highest phenolic content and presenting the most heterogenous proanthocyanidin composition. Further, cytotoxicity of leaves extracts in two cancer cell lines was also found to be higher than in the remaining parts of the plant. In the present study, fractioning of U. tomentosa leaves polyphenolic extracts was performed using Diaion® HP-20 resin and a detailed characterization and quantification of fractions (n = 5) was achieved using advanced analytical techniques such as Ultra-Performance Liquid Chromatography coupled with Electrospray Ionization and Triple Quadrupole (TQD) Tandem Mass Spectrometry (UPLC/TQ-ESI-MS) and 13C-NMR. Oxygen Radical Absorbance Capacity (ORAC) and cytotoxicity on gastric adenocarcinoma AGS and colon adenocarcinoma SW20 cell lines were also determined in the different fractions. Results showed selective distribution of 32 non-flavonoid and flavonoid phenolics among the different fractions. ORAC varied between 3.2 and 11.8 µmol TE/mg in the different fractions, whereas IC50 of cytotoxicity on gastric adenocarcinoma AGS and colon adenocarcinoma SW20 cell lines best values were between 71.4 and 75.6 µg/mL. Fractions rich in proanthocyanidins also showed the highest bioactivity. In fact, significant positive correlation was found between total proanthocyanidins (TP) quantified by UPLC-DAD and ORAC (R 2 = 0.970), whereas significant negative correlation was found between TP and cytotoxicity towards AGS (R 2 = 0.820) and SW620 (R 2 = 0.843) adenocarcinoma cell lines. Among proanthocyanidins, propelargonidin dimers were of particular interest, showing significant correlation with cytotoxic selectivity on both gastric AGS (R 2 = 0.848) and colon SW620 (R 2 = 0.883) adenocarcinoma cell lines. These results show further evidence of the bioactivity of U. tomentosa proanthocyanidin extracts and their potential health effects.Agencia Española de Cooperación Internacional para el Desarrollo/[A/023397/09]/AECID/EspañaAgencia Española de Cooperación Internacional para el Desarrollo/[A/030037/10]/AECID/EspañaCosta Rica-USA Foundation/[]/CRUSA/Costa RicaConsejo Superior de Investigaciones Científicas/[CR0024]/CSIC/EspañaUniversidad de Costa Rica/[]/UCR/Costa RicaUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de QuímicaUCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de MedicinaUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologí

Sedimentological and palaeohydrological characterization of Late Pleistocene and Holocene tufa mound palaeolakes using trenching methods in the Spanish Pyrenees

Lakes developed in the inner depressions of tufa mounds are rare geomorphic features and still poorly understood. Sedimentation in this unusual type of endorheic lake with a very restricted catchment area is highly sensitive to environmental and hydrological changes. The Isona tufa mound complex, north-eastern Iberian Peninsula, is associated with the discharge zone of a confined artesian aquifer and comprises 11 tufa mounds consisting of an annular rimstone enclosing a central depression filled with lake deposits. Data gathered from trenches excavated in four palaeolakes located within three different morphostratigraphic units permitted a precise analysis of the geometrical characteristics and stratigraphic relationships of the deposits and provided a sedimentation model for the Late Quaternary infilling of the spring-fed lakes. The work illustrates that trenches allow a precise characterization of the stratigraphic arrangements, lateral facies changes and deformation structures, which are not apparent in studies relying solely on borehole records, and facilitate sampling for dating and geochemical analyses. The five sedimentary facies described represent different evolutionary stages of the lakes, including: (i) carbonate-rich palustrine deposits probably related to periods with strong hydrological seasonality; (ii) massive highly bioturbated organic ooze; (iii) banded organic carbonate-rich facies associated with an increase in the regional effective moisture; (iv) finegrained quartz-rich aeolian/slope-wash sediments; and (v) colluvial facies deposited following the desiccation of the lakes located at higher altitudes. Geochemical and sedimentological analyses of the lacustrine sequences provided information on the palaeohydrological evolution of the Isona tufa mound complex and the palaeoenvironmental conditions of the area over the last 28 ka. Radiometric dating suggests that deposition occurred simultaneously at ca 22 ka in palaeolakes situated at different elevations. A drop in the piezometric level prompted by the opening of springs at lower altitudes probably caused the deactivation of the upper springs and the desiccation of the lakes. Arid conditions prevailed in the area during the Late Glacial and the early Holocene (28·0 to 8·5 ka BP). More humid conditions recorded from 8·5 to 4·2 ka and again since 1·7 ka are in accordance with palaeoenvironmental reconstructions available in the Western Mediterranean since the Last Glacial Maximum

Ground-State Spin Blockade in a Single-Molecule Junction

It is known that the quantum mechanical ground state of a nanoscale junction has a significant impact on its electrical transport properties. This becomes particularly important in transistors consisting of a single molecule. Because of strong electron-electron interactions and the possibility of accessing ground states with high spins, these systems are eligible hosts of a current-blockade phenomenon called a ground-state spin blockade. This effect arises from the inability of a charge carrier to account for the spin difference required to enter the junction, as that process would violate the spin selection rules. Here, we present a direct experimental demonstration of a ground-state spin blockade in a high-spin single-molecule transistor. The measured transport characteristics of this device exhibit a complete suppression of resonant transport due to a ground-state spin difference of 3/2 between subsequent charge states. Strikingly, the blockade can be reversibly lifted by driving the system through a magnetic ground-state transition in one charge state, using the tunability offered by both magnetic and electric fields