Dosaggio densitometrico del 6beta-idrossi-cortisolo nelle urine

4nonenonePiero, Corti; Clizia, Murratzu; Gianfranco, Sciarra; Corbini, GianfrancoPiero, Corti; Clizia, Murratzu; Gianfranco, Sciarra; Corbini, Gianfranc

Determination of the Modified “Affinity Index” of Small Ligands and Macromolecular Receptors from NMR Spin-lattice Relaxation Data

In this Letter we propose a development of a NMR methodology to study ligand–macromolecular receptor interactions, based on the analysis of proton selective spin-lattice relaxation rate enhancements of the ligand, to calculate an affinity index, related to strength of the interaction process. In this work we modified this index by normalization to the relaxation rate of the free ligand, in order to take into account the effects of motional anisotropies and different proton densities. This approach also allowed the calculation of the relaxation rate of the bound ligand as well as the equilibrium constant of the interaction process

Quantitative analysis of Lamotrigine in plasma and tablets by planar chromatography and comparison with liquid

A method using planar chromatography (PC) was developed for determining lamotrigine (LTG; 3,5-diamino-6-[2,3-dichlorophenyl]-1,2,4-triazine) in human plasma and tablets. LTG was extracted with acetonitrile in the presence of sodium carbonate. 3,5-Diamino-6-(2-methoxyphenyl)-1,2,4-triazine was used as internal standard, The detection limit was 0.27 mu g/mL plasma and the recovery from human plasma fortified with various concentrations of LTG was 91.3 +/- 3.4%. Interference from other common antiepileptic agents was checked. Results obtained with the PC method were compared with those obtained by a method using liquid chromatography (LC) for analysis of plasma and with methods using LC and UV for assay of tablets