Atom Interferometric Imaging of Differential Potentials Using an Atom Laser

Interferometry is a prime technique for modern precision measurements. Atoms, unlike light, have significant interactions with electric, magnetic, and gravitational fields, making their use in interferometric applications particularly versatile. Here, we demonstrate atom interferometry to image optical and magnetic potential landscapes over an area exceeding $240 \mu m \times 600 \mu m$. The differential potentials employed in our experiments generate phase imprints in an atom laser that are made visible through a Ramsey pulse sequence. We further demonstrate how advanced pulse sequences can enhance desired imaging features, e.g. to image steep potential gradients. A theoretical discussion is presented that provides a semiclassical analysis and matching numerics.Comment: Updated to match published version. 13 pages, 9 figure

Surface Brightness Profiles of Composite Images of Compact Galaxies at z~4-6 in the HUDF

The Hubble Ultra Deep Field (HUDF) contains a significant number of B, V and i'-band dropout objects, many of which were recently confirmed to be young star-forming galaxies at z~4-6. These galaxies are too faint individually to accurately measure their radial surface brightness profiles. Their average light profiles are potentially of great interest, since they may contain clues to the time since the onset of significant galaxy assembly. We separately co-add V, i' and z'-band HUDF images of sets of z~4,5 and 6 objects, pre-selected to have nearly identical compact sizes and the roundest shapes. From these stacked images, we are able to study the averaged radial structure of these objects at much higher signal-to-noise ratio than possible for an individual faint object. Here we explore the reliability and usefulness of a stacking technique of compact objects at z~4-6 in the HUDF. Our results are: (1) image stacking provides reliable and reproducible average surface brightness profiles; (2) the shape of the average surface brightness profiles show that even the faintest z~4-6 objects are resolved; and (3) if late-type galaxies dominate the population of galaxies at z~4-6, as previous HST studies have shown, then limits to dynamical age estimates for these galaxies from their profile shapes are comparable with the SED ages obtained from the broadband colors. We also present accurate measurements of the sky-background in the HUDF and its associated 1-sigma uncertainties.Comment: 10 pages, 9 figures, 2 tables, emulateapj; Accepted for publication in The Astronomical Journa

Planning for climate change impacts: coastal tourism destination resilience policies

Coastal tourism is a vital part of the world economy bit also facilitates access to “blue spaces” – this offers significant health benefits to populations and shapes our relationship with the coast. At present, climate change impacts on the coast, such as storm surges and rising sea levels, are an inevitability and, in some regions, they are already damaging coastal tourism economies. It is clear, therefore, that our relationship with the coast will change. Given the importance of coastal tourism, this new relationship needs to incorporate adaptive measures; including a blue space and wellbeing focus, education and awareness of challenges facing the natural environment. We make this case by re-evaluating the literature on coastal tourism and climate adaptation policies. Using recent examples, we also offer an indication of new policy directions for coastal destinations. At present coastal tourism plans and policies largely ignore how destinations might respond and adapt to climate change impacts. We critique this and suggest that the human–environment relationship should be placed directly at the centre of all thinking on this topic

‘We had the most wonderful times’: seaside nostalgia at a British resort

Morecambe is a traditional British seaside resort that experienced a dynamic ebb and flow of visitors. It still attracts visitors, many of whom are from the North of England and in the second half of their lives. The experiences of such traditional seaside markets have not been examined as carefully by academics in recent years as one might assume. All too often this subject falls between the gap between serious academic study and popular culture, which supports narratives focussing on the apparent decline of an idealised seaside. Instead this paper attempts to gain an understanding of this seaside experience, and is based around ten semi-structured interviews with 55-74 year old repeat North of England visitors to Morecambe. It considers their nostalgic connection and reaction to the resort, which emerged as a significant element of visitor experience. The seaside is considered timeless by these visitors and facilitates a reverie through which one can temporarily revisit a past which is populated by childhood memories of family members. The resort allows visitors to fleetingly transcend time, through immersion in the unchanging resort with its timeless seacape. This reconnection with the past highlights a dissatisfaction with the present which hinges on the loss of childhood. Yet nostalgia also allowed for a positive re-telling of the past which underpinned family narratives and contributed to the cross-generational appeal of the beach

Pan-Cancer Analysis of lncRNA Regulation Supports Their Targeting of Cancer Genes in Each Tumor Context

Long noncoding RNAs (lncRNAs) are commonly dys-regulated in tumors, but only a handful are known toplay pathophysiological roles in cancer. We inferredlncRNAs that dysregulate cancer pathways, onco-genes, and tumor suppressors (cancer genes) bymodeling their effects on the activity of transcriptionfactors, RNA-binding proteins, and microRNAs in5,185 TCGA tumors and 1,019 ENCODE assays.Our predictions included hundreds of candidateonco- and tumor-suppressor lncRNAs (cancerlncRNAs) whose somatic alterations account for thedysregulation of dozens of cancer genes and path-ways in each of 14 tumor contexts. To demonstrateproof of concept, we showed that perturbations tar-geting OIP5-AS1 (an inferred tumor suppressor) andTUG1 and WT1-AS (inferred onco-lncRNAs) dysre-gulated cancer genes and altered proliferation ofbreast and gynecologic cancer cells. Our analysis in-dicates that, although most lncRNAs are dysregu-lated in a tumor-specific manner, some, includingOIP5-AS1, TUG1, NEAT1, MEG3, and TSIX, synergis-tically dysregulate cancer pathways in multiple tumorcontexts

Genomic, Pathway Network, and Immunologic Features Distinguishing Squamous Carcinomas

This integrated, multiplatform PanCancer Atlas study co-mapped and identified distinguishing molecular features of squamous cell carcinomas (SCCs) from five sites associated with smokin

Pan-cancer Alterations of the MYC Oncogene and Its Proximal Network across the Cancer Genome Atlas

Although theMYConcogene has been implicated incancer, a systematic assessment of alterations ofMYC, related transcription factors, and co-regulatoryproteins, forming the proximal MYC network (PMN),across human cancers is lacking. Using computa-tional approaches, we define genomic and proteo-mic features associated with MYC and the PMNacross the 33 cancers of The Cancer Genome Atlas.Pan-cancer, 28% of all samples had at least one ofthe MYC paralogs amplified. In contrast, the MYCantagonists MGA and MNT were the most frequentlymutated or deleted members, proposing a roleas tumor suppressors.MYCalterations were mutu-ally exclusive withPIK3CA,PTEN,APC,orBRAFalterations, suggesting that MYC is a distinct onco-genic driver. Expression analysis revealed MYC-associated pathways in tumor subtypes, such asimmune response and growth factor signaling; chro-matin, translation, and DNA replication/repair wereconserved pan-cancer. This analysis reveals insightsinto MYC biology and is a reference for biomarkersand therapeutics for cancers with alterations ofMYC or the PMN

Spatial Organization and Molecular Correlation of Tumor-Infiltrating Lymphocytes Using Deep Learning on Pathology Images

Beyond sample curation and basic pathologic characterization, the digitized H&E-stained images of TCGA samples remain underutilized. To highlight this resource, we present mappings of tumorinfiltrating lymphocytes (TILs) based on H&E images from 13 TCGA tumor types. These TIL maps are derived through computational staining using a convolutional neural network trained to classify patches of images. Affinity propagation revealed local spatial structure in TIL patterns and correlation with overall survival. TIL map structural patterns were grouped using standard histopathological parameters. These patterns are enriched in particular T cell subpopulations derived from molecular measures. TIL densities and spatial structure were differentially enriched among tumor types, immune subtypes, and tumor molecular subtypes, implying that spatial infiltrate state could reflect particular tumor cell aberration states. Obtaining spatial lymphocytic patterns linked to the rich genomic characterization of TCGA samples demonstrates one use for the TCGA image archives with insights into the tumor-immune microenvironment

A Practical Guide to Rodent Islet Isolation and Assessment

Pancreatic islets of Langerhans secrete hormones that are vital to the regulation of blood glucose and are, therefore, a key focus of diabetes research. Purifying viable and functional islets from the pancreas for study is an intricate process. This review highlights the key elements involved with mouse and rat islet isolation, including choices of collagenase, the collagenase digestion process, purification of islets using a density gradient, and islet culture conditions. In addition, this paper reviews commonly used techniques for assessing islet viability and function, including visual assessment, fluorescent markers of cell death, glucose-stimulated insulin secretion, and intracellular calcium measurements. A detailed protocol is also included that describes a common method for rodent islet isolation that our laboratory uses to obtain viable and functional mouse islets for in vitro study of islet function, beta-cell physiology, and in vivo rodent islet transplantation. The purpose of this review is to serve as a resource and foundation for successfully procuring and purifying high-quality islets for research purposes