714 research outputs found
Creating tissue on chip constructs in microtitre plates for drug discovery
We report upon a novel coplanar dielectrophoresis (DEP) based cell patterning system for generating transferrable hepatic cell constructs, resembling a liver-lobule, in culture. The use of paper reinforced gel substrates provided sufficient strength to enable these constructs to be transfered into 96-well plates for long term functional studies, including in the future, drug development studies. Experimental results showed that hepatic cells formed DEP field-induced structures corresponding to an array of lobule-mimetic patterns. Hepatic viability was observed over a period of 3 days by the use of a fluorescent cell staining technique, whilst the liver specific functionality of albumin secretion showed a significant enhancement due to the layer patterning of cell lines (HepG2/C3A), compared to 2D patterned cells and un-patterned control. This âbuild and transferâ concept could, in future, also be adapted for the layer-by-layer construction of organs-on-chip in microtitre formats
Vibration hotspots reveal longitudinal funneling of sound-evoked motion in the mammalian cochlea
The micromechanical mechanisms that underpin tuning and dynamic range compression in the mammalian inner ear are fundamental to hearing, but poorly understood. Here, we present new, high-resolution optical measurements that directly map sound-evoked vibrations on to anatomical structures in the intact, living gerbil cochlea. The largest vibrations occur in a tightly delineated hotspot centering near the interface between the Deitersâ and outer hair cells. Hotspot vibrations are less sharply tuned, but more nonlinear, than basilar membrane vibrations, and behave non-monotonically (exhibiting hyper-compression) near their characteristic frequency. Amplitude and phase differences between hotspot and basilar membrane responses depend on both frequency and measurement angle, and indicate that hotspot vibrations involve longitudinal motion. We hypothesize that structural coupling between the Deitersâ and outer hair cells funnels sound-evoked motion into the hotspot region, under the control of the outer hair cells, to optimize cochlear tuning and compression
Quantum field dynamics of the slow rollover in the linear delta expansion
We show how the linear delta expansion, as applied to the slow-roll
transition in quantum mechanics, can be recast in the closed time-path
formalism. This results in simpler, explicit expressions than were obtained in
the Schr\"odinger formulation and allows for a straightforward generalization
to higher dimensions. Motivated by the success of the method in the
quantum-mechanical problem, where it has been shown to give more accurate
results for longer than existing alternatives, we apply the linear delta
expansion to four-dimensional field theory.
At small times all methods agree. At later times, the first-order linear
delta expansion is consistently higher that Hartree-Fock, but does not show any
sign of a turnover. A turnover emerges in second-order of the method, but the
value of at the
turnover. In subsequent applications of the method we hope to implement the
calculation in the context of an expanding universe, following the line of
earlier calculations by Boyanovsky {\sl et al.}, who used the Hartree-Fock and
large-N methods. It seems clear, however, that the method will become
unreliable as the system enters the reheating stage.Comment: 17 pages, 9 figures, revised version with extra section 4.2 including
second order calculatio
Signatures of Electronic Nematic Phase at Isotropic-Nematic Phase Transition
The electronic nematic phase occurs when the point-group symmetry of the
lattice structure is broken, due to electron-electron interactions. We study a
model for the nematic phase on a square lattice with emphasis on the phase
transition between isotropic and nematic phases within mean field theory. We
find the transition to be first order, with dramatic changes in the Fermi
surface topology accompanying the transition. Furthermore, we study the
conductivity tensor and Hall constant as probes of the nematic phase and its
transition. The relevance of our findings to Hall resistivity experiments in
the high- cuprates is discussed.Comment: 5 pages, 3 figure
The frequency limit of outer hair cell motility measured in vivo
Outer hair cells (OHCs) in the mammalian ear exhibit electromotility, electrically driven somatic length changes that are thought to mechanically amplify sound-evoked vibrations. For this amplification to work, OHCs must respond to sounds on a cycle-by-cycle basis even at frequencies that exceed the low-pass corner frequency of their cell membranes. Using in vivo optical vibrometry we tested this theory by measuring sound-evoked motility in the 13-25 kHz region of the gerbil cochlea. OHC vibrations were strongly rectified, and motility exhibited first-order low-pass characteristics with corner frequencies around 3 kHz- more than 2.5 octaves below the frequencies the OHCs are expected to amplify. These observations lead us to suggest that the OHCs operate more like the envelope detectors in a classical gain-control scheme than like high-frequency sound amplifiers. These findings call for a fundamental reconsideration of the role of the OHCs in cochlear function and the causes of cochlear hearing loss
Vanishing Hall Constant in the Stripe Phase of Cuprates
The Hall constant R_H is considered for the stripe structures. In order to
explain the vanishing of R_H in LNSCO at x = 1/8, we use the relation of R_H to
the Drude weight D as well as direct numerical calculation, to obtain results
within the t-J model, where the stripes are imposed via a charge potential and
a staggered magnetic field. The origin of R_H ~ 0 is related to a maximum in D
and the minimal kinetic energy in stripes with a hole filling ~ 1/2. The same
argument indicates on a possibility of R_H ~ 0 in the whole range of static
stripes for x < 1/8.Comment: RevTeX, 4 pages, 5 figure
Closed-Time Path Integral Formalism and Medium Effects of Non-Equilibrium QCD Matter
We apply the closed-time path integral formalism to study the medium effects
of non-equilibrium gluon matter. We derive the medium modified resummed gluon
propagator to the one loop level in non-equilibrium in the covariant gauge. The
gluon propagator we derive can be used to remove the infrared divergences in
the secondary parton collisions to study thermalization of minijet parton
plasma at RHIC and LHC.Comment: Final version, To appear in Physical Review D, Minor modification,
reference adde
Time capsules of biodiversity: Future research directions for groundwater-dependent ecosystems of the Great Artesian Basin
The Great Artesian Basin of Australia represents one of the largest and deepest basins of freshwater on Earth. Thousands of springs fed by the Basin are scattered across Australiaâs arid zone, often representing the sole sources of freshwater for thousands of kilometers. As âislandsâ in the desert, the springs support endemic fauna and flora that have undergone millions of years of evolution in almost total isolation. Here, we review the current body of knowledge surrounding Great Artesian Basin springs and their significance from ecological, evolutionary, and cultural perspectives using South Australian spring wetlands as a case study. We begin by identifying the status of these springs as critical sources of groundwater, the unique biodiversity they support, and their cultural significance to the Arabana people as Traditional Custodians of the land. We then summarize known threats to the springs and their biota, both exogenous and endogenous, and the potential impacts of such processes. Finally, considering the status of these at-risk habitats as time capsules of biodiversity, we discuss lessons that can be learnt from current conservation and management practices in South Australia. We propose key recommendations for improved biodiversity assessment and monitoring of Great Artesian Basin springs nationwide, including 1) enhanced legal protections for spring biota; 2) increased taxonomic funding and capacity; 3) improved biodiversity monitoring methods, and 4) opportunities for reciprocal knowledge-sharing with Aboriginal peoples when conducting biodiversity research.P. G. Beasley-Hall, N. P. Murphy, R. A. King, N. E. White, B. A. Hedges, S. J. B. Cooper, A. D. Austin, and M. T. Guzi
Anomalous Pseudoscalar-Photon Vertex In and Out of Equilibrium
The anomalous pseudoscalar-photon vertex is studied in real time in and out
of equilibrium in a constituent quark model. The goal is to understand the
in-medium modifications of this vertex, exploring the possibility of enhanced
isospin breaking by electromagnetic effects as well as the formation of neutral
pion condensates in a rapid chiral phase transition in peripheral,
ultrarelativistic heavy-ion collisions. In equilibrium the effective vertex is
afflicted by infrared and collinear singularities that require hard thermal
loop (HTL) and width corrections of the quark propagator. The resummed
effective equilibrium vertex vanishes near the chiral transition in the chiral
limit. In a strongly out of equilibrium chiral phase transition we find that
the chiral condensate drastically modifies the quark propagators and the
effective vertex. The ensuing dynamics for the neutral pion results in a
potential enhancement of isospin breaking and the formation of
condensates. While the anomaly equation and the axial Ward identity are not
modified by the medium in or out of equilibrium, the effective real-time
pseudoscalar-photon vertex is sensitive to low energy physics.Comment: Revised version to appear in Phys. Rev. D. 42 pages, 4 figures, uses
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