Inverse expression of P k and Luke blood group antigens on human RBCs

Luke (LKE) is a high-frequency RBC antigen, related to the P blood group system. A LKE-negative phenotype is found in 1 to 2 percent of donors and may be associated with increased P k . Because P k and similar glycolipids are receptors for shiga toxin on cell membranes, a LKE-negative phenotype could have implications for infections by Shigella dysenteriae and enterohemorrhagic Escherichia coli . STUDY DESIGN AND METHODS: Volunteer donors (n = 257) were serologically typed for LKE with a LKE MoAb, MC813-70. LKE-strong-positive, LKE-weak-positive and LKE-negative RBCs were analyzed for P k , P, LKE, and shiga toxin binding by immunofluorescence flow cytometry, high-performance thin-layer chromatography, scanning densitometry, and high-performance thin-layer chromatography immunostaining. RESULTS: Among Iowa donors, 78.6 percent were LKE-strong-positive, 20.2 percent were LKE-weak-positive, and 1.2 percent were LKE-negative. There was an inverse expression of P k and LKE on RBCs. P k expression was increased on LKE-negative RBCs and was associated with increased shiga toxin binding. A LKE-active glycolipid was identified in the ganglioside fraction of LKE-strong-positive RBCs. CONCLUSION: A LKE-negative phenotype is associated with increased expression of P k on RBCs. Differences in P k and LKE expression may play a role in host susceptibility to infection with S. dysenteriae and E. coli .Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73915/1/j.1537-2995.2001.41070898.x.pd

Red blood cell transfusion in palliative care: what are we doing and why are we doing it?

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141052/1/trf14437.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/141052/2/trf14437_am.pd

An evaluation of storage time for dithiothreitol‐treated reagent cells

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/138313/1/trf14244.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/138313/2/trf14244_am.pd

Acquired hemoglobin C secondary to transfusion with antigen‐matched red blood cells

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/107569/1/jca21306.pd

Anti-A and anti-B titers in pooled group O platelets are comparable to apheresis platelets

Although uncommon, acute hemolytic transfusion reactions (AHTRs) have been reported after transfusion of group O single-donor apheresis platelets (SDPs) to group A, B, and AB recipients. Current methods for identifying “high-titer” SDPs include tube and gel methods. The risk of a high-titer unit is considered low with group O, poststorage, pooled platelet concentrates (PPLTs); however, data regarding anti-A and anti-B titers in PPLTs are lacking. STUDY DESIGN AND METHODS: Anti-A and anti-B titers were determined in 185 PPLTs by direct agglutination using manual gel and tube methods. PPLTs tested included 124 group O PPLTs, 25 group A PPLTs, 26 group B PPLTs, and 10 PPLTs containing a mix of either groups O plus A or groups O plus B (mixed PPLTs). The reciprocal of the highest dilution giving macroscopic agglutination was considered the agglutinin titer. RESULTS: Mean anti-A and anti-B titers in group O PPLTs were, respectively, 16 and 8 by tube and 64 and 32 by gel (p < 0.0001). Gel titers were one to two dilutions higher than tube and sensitive to reagent red cell lots. With the use of at least 64 as a critical titer, 60 percent of group O PPLTs tested by gel would be considered high-titer. In mixed PPLTs, the addition of one non-group O PLT significantly decreased or neutralized the corresponding anti-A or anti-B (p < 0.0001). CONCLUSION: Anti-A and anti-B titers in group O PPLTs are comparable to those reported in group O SDPs and significantly lower than titers reported in AHTR. A critical direct agglutinin titer of 64 for identifying high-titer units by gel is too low and should be increased to 128 or higher.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73545/1/j.1537-2995.2008.01814.x.pd

Kids, colds, and complement: paroxysmal cold hemoglobinuria

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/137341/1/trf14128.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/137341/2/trf14128_am.pd