13 research outputs found

    Chokwe! Art and Initiation among Chokwe and Related Peoples

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    New Acquisitions of African Art at the Cleveland Museum of Art

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    Bwadi Bwa Chikwanga: A Ram Mask of the Bakwa Luntu

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    What Does It Mean to Keep Kissing-Close to the Evidence, and Why Might It Matter?

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    African art specialists often lack detailed information to assess the original meanings, uses, and contexts of so-called historical or traditional arts of Africa, and they rely on indirect evidence to interpret the works. Thus, claims about African arts often reflect speculation rather than irrefutable details. When specific documentation for an object does exist, the circumstances of its creation require careful evaluation as well. The assessment of the quality and reliability of any claim is of particular importance in attempts to determine an object’s place of origin in the ongoing debates about restitution

    Exploring Parametric and Mechanistic Differences between Expi293F<sup>TM</sup> and ExpiCHO-S<sup>TM</sup> Cells for Transient Antibody Production Optimization

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    Rapidly producing drug-like antibody therapeutics for lead molecule discovery and candidate optimization is typically accomplished by large-scale transient gene expression technologies (TGE) with cultivated mammalian cells. The TGE methodologies have been extensively developed over the past three decades, yet produce significantly lower yields than the stable cell line approach, facing the technical challenge of achieving universal high expression titers for a broad range of antibodies and therapeutics modalities. In this study, we explored various parameters for antibody production in the TGE cell host Expi293FTM and ExpiCHO-STM with the transfection reagents ExpiFectamineTM and polyethylenimine. We discovered that there are significant differences between Expi293FTM and ExpiCHO-STM cells with regards to DNA complex formation time and ratio, complex formation buffers, DNA complex uptake trafficking routes, responses to dimethyl sulfoxide and cell cycle inhibitors, as well as light-chain isotype expression preferences. This investigation mechanistically dissected the TGE processes and provided a new direction for future transient antibody production optimization
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