Epidemiology and molecular typing of multidrug-resistant bacteria in day care centres in Flanders, Belgium

The global prevalence and spread of multidrug-resistant organisms (MDROs) represent an emerging public health threat. Day care centre (DCC) attendance is a risk factor for MDRO carriage in children and their environment. This study aimed to map the epidemiology of carriage and potential transmission of these organisms within 18 Flemish DDCs (Belgium). An MDRO prevalence survey was organised between November 2018 and February 2019 among children attending the centres. Selective chromogenic culture media were used for the detection of extended-spectrum beta-lactamase-producing Enterobacterales (ESBL-E), carbapenemase-producing Enterobacterales (CPE), and vancomycin-resistant Enterococci (VRE) in faecal swabs obtained from diapers or jars (n = 448). All isolated MDROs were subjected to resistance gene sequencing. A total of 71 of 448 samples (15.8%) yielded isolates of ESBL-E with a predominance of Escherichia coli (92.2% of ESBL-E) and ESBL resistance gene blaCTX-M-15 (50.7% of ESBL coding genes in E. coli). ESBL-E prevalence varied between DCCs, ranging from 0 to 50%. Transmission, based on the clonal relatedness of ESBL-E strains, was observed. CPE was identified in only one child carrying an E. coli with an OXA-244 gene. VRE was absent from all samples. The observed prevalence of ESBL-E in Flemish DCCs is high compared with previous studies, and our findings re-emphasise the need for rigorous hygiene measures within such centres to control the further spread of MDROs in the community

Epidemiology and molecular typing of multidrug-resistant bacteria in tertiary hospitals and nursing homes in Flanders, Belgium

This study aimed to map MDRO carriage and potential transmission within and between three Flemish tertiary care hospitals and their neighbouring nursing homes. A cross-sectional MDRO prevalence survey was organized between October 2017 and February 2019. Perianal swabs were cultured for detection of MDRO. Determination of clonal relatedness based on wgMLST allelic profiles was performed. The prevalence of MDRO in Belgian hospitals and NHs is on the rise, compared to previous studies, and transmission in and between institutions is observed. These results re-emphasize the need for a healthcare network-wide infection prevention strategy in which WGS of MDRO strains can be supportive

Assessing the effect of a classroom IEQ on student satisfaction, engagement and performance

Inappropriate indoor environmental quality (IEQ) conditions are shown to reduce occupants’ satisfaction, wellbeing and performance. Therefore, it is crucial to provide an excellent classroom IEQ in order to minimize learning loss among students. This study determines the effect of different thermal and indoor air quality (IAQ) conditions in a classroom on students’ satisfaction, study engagement and cognitive performance. Three data collection campaigns were performed in a university classroom. Data collection consisted of (1) continuous IEQ monitoring (i.e., temperature, relative humidity, CO2, …), (2) frequent assessments of students’ satisfaction with IEQ and study engagement via here-and-now surveys, and (3) evaluations of students’ memory and processing speed. The IEQ conditions were varied by adapting (1) a room temperature setpoint between 18°C to 26°C and (2) a CO2-setpoint between 500 ppm and 1000 ppm. A mixed-effects regression analysis on the collected data showed statistically significant relationships between students’ study engagement and the room temperature trend, air enthalpy, relative humidity and TVOC-concentrations in the classroom. The addition of data on students’ satisfaction with IEQ only improved a minority of model fits. A statistically significant difference in students’ memory was found between test conditions. However, a randomized experimental design is needed to determine the relationship between the classroom IEQ and students’ cognitive performance

Expertsysteem voor het ontwerpen van spuitgietmatrijzen met standaardcomponenten

SIGLEKULeuven Campusbibliotheek Exacte Wetenschappen / UCL - Université Catholique de LouvainBEBelgiu