Validation of a comercially available fluorescence-based instrument to evaluate stallion spermatozoal concentration and comparison to photometric systems

Accurate measurement of stallion spermatozoal concentration is important to equine breeding operations. The hemacytometer is considered the standard for measuring spermatozoal concentration but is time consuming and may be imprecise. The flow cytometer is considered precise and accurate, but only practical for research purposes due to sample preparation time and high cost. Photometric systems are commonly used but can be inaccurate outside a relatively narrow concentration range and can be rendered inaccurate in the presence of contaminants. A new instrument, the NucleoCounter SP-100 is reported to enumerate spermatozoa at wider concentration ranges and can identify spermatozoa in opaque semen extenders. Epididymal, neat (raw) ejaculates, and ejaculates diluted in various semen extenders were analyzed with the NucleoCounter, the Densimeter, the Spermacue, flow cytometric and hemacytometric methods. Results were compared statistically by: 1) regression analysis, 2) the agreement of two instruments, whereby the difference in values between two instruments was plotted on the y-axis against the mean of those values on the x-axis [26] and 3) a modified method that measured the percentage deviation, whereby the percentage (of the difference in values between two instruments divided by the mean) of the same two values was plotted on the y-axis against the mean value of the two instruments on the x-axis. The NucleoCounter showed more agreement with both the flow cytometer and hemacytometer for epididymal, neat ejaculated and extended spermatozoa over a range of concentrations than the Densimeter or the Spermacue. The NucleoCounter showed more agreement with the flow cytometer for epididymal and neat ejaculated spermatozoa and more agreement with the hemacytometer for spermatozoa diluted in semen extenders. The Spermacue showed the least agreement with both standards for all spermatozoal comparisons. All coefficients of variation for the flow cytometer, hemacytometer and NucleoCounter were >10% for all spermatozoal comparisons. This study indicates that the NucleoCounter shows more agreement with the flow cytometer and hemacytometer than photometric systems when evaluated with epididymal, neat ejaculated and extended spermatozoa. The instrument is also more repeatable than either photometric system, but may be cost-prohibitive for some operations

Extended X-ray Emission From a Quasar-Driven Superbubble

We present observations of extended, 20-kpc scale soft X-ray gas around a luminous obscured quasar hosted by an ultra-luminous infrared galaxy caught in the midst of a major merger. The extended X-ray emission is well fit as a thermal gas with a temperature of kT ~ 280 eV and a luminosity of L_X ~ 10^42 erg/s and is spatially coincident with a known ionized gas outflow. Based on the X-ray luminosity, a factor of ~10 fainter than the [OIII] emission, we conclude that the X-ray emission is either dominated by photoionization, or by shocked emission from cloud surfaces in a hot quasar-driven wind.Comment: Accepted for publication in ApJ, 6 pages, 2 figure

Linking Environmental Sustainability and Healthcare: The Effects of an Energy Saving Intervention in Two Hospitals

Set in a real organisational setting, this study examines the challenges of implementing environmentally sustainable behaviour in healthcare. It evaluates the success of a real energy saving behaviour change intervention, based on social marketing principles, which targeted the employees of two National Health Service (NHS) hospitals. It also explores the intervention benefits for three key stakeholders: the organisation/hospitals, hospital employees and patients. A rich secondary dataset containing actual workplace behaviour measures (collected via observations) and self-reported data from employee interviews and patient questionnaires is used for this purpose. The intervention encouraged three employee energy saving actions (called TLC actions): (1) Turn off machines, (2) Lights out when not needed, and (3) Close doors when possible; which led to energy savings and carbon reduction for the two hospitals. Hospital employees reported a greater level of work efficiency as a result of engaging in TLC actions, which increased the 'quiet time' periods in both hospitals. Indirectly, employees' TLC actions also improved patients' quality of sleep (which in turn is positively associated with greater patient hospital experience satisfaction). These findings shed light on the benefits of social marketing interventions targeting energy saving behaviour change for multiple stakeholders in healthcare organisations. They also illustrate connections between environmental sustainability and social and political pillars of corporate social responsibility. Additionally, organisational culture was highlighted as a key challenge in changing practices. To encourage long-term sustainable behaviour, this study recommends a pre-intervention assessment of infrastructure and equipment, the communication of expected benefits to motivate higher involvement of employees, the need for internal green champions and the dissemination of post-intervention feedback on various energy saving and patient indicators

A Search for Binary Active Galactic Nuclei: Double-Peaked [OIII] AGN in the Sloan Digital Sky Survey

We present AGN from the Sloan Digital Sky Survey (SDSS) having double-peaked profiles of [OIII] 5007,4959 and other narrow emission-lines, motivated by the prospect of finding candidate binary AGN. These objects were identified by means of a visual examination of 21,592 quasars at z < 0.7 in SDSS Data Release 7 (DR7). Of the spectra with adequate signal-to-noise, 148 spectra exhibit a double-peaked [OIII] profile. Of these, 86 are Type 1 AGN and 62 are Type 2 AGN. Only two give the appearance of possibly being optically resolved double AGN in the SDSS images, but many show close companions or signs of recent interaction. Radio-detected quasars are three times more likely to exhibit a double-peaked [OIII] profile than quasars with no detected radio flux, suggesting a role for jet interactions in producing the double-peaked profiles. Of the 66 broad line (Type 1) AGN that are undetected in the FIRST survey, 0.9% show double peaked [OIII] profiles. We discuss statistical tests of the nature of the double-peaked objects. Further study is needed to determine which of them are binary AGN rather than disturbed narrow line regions, and how many additional binaries may remain undetected because of insufficient line-of-sight velocity splitting. Previous studies indicate that 0.1% of SDSS quasars are spatially resolved binaries, with typical spacings of ~10 to 100 kpc. If a substantial fraction of the double-peaked objects are indeed binaries, then our results imply that binaries occur more frequently at smaller separations (< 10 kpc). This suggests that simultaneous fueling of both black holes is more common as the binary orbit decays through these spacings.Comment: 33 pages, 5 figures, LaTeX. Major revisions. Accepted for publication in ApJ

Morphologies of Radio, X-Ray, and Mid-Infrared Selected AGN

We investigate the optical morphologies of candidate active galaxies identified at radio, X-ray, and mid-infrared wavelengths. We use the Advanced Camera for Surveys General Catalog (ACS-GC) to identify 372, 1360, and 1238 AGN host galaxies from the VLA, XMM-Newton and Spitzer Space Telescope observations of the COSMOS field, respectively. We investigate both quantitative (GALFIT) and qualitative (visual) morphologies of these AGN host galaxies, split by brightness in their selection band. We find that the radio-selected AGN are most distinct, with a very low incidence of having unresolved optical morphologies and a high incidence of being hosted by early-type galaxies. In comparison to X-ray selected AGN, mid-IR selected AGN have a slightly higher incidence of being hosted by disk galaxies. These morphological results conform with the results of Hickox et al. 2009 who studied the colors and large-scale clustering of AGN, and found a general association of radio-selected AGN with ``red sequence'' galaxies, mid-IR selected AGN with ``blue cloud'' galaxies, and X-ray selected AGN straddling these samples in the ``green valley.'' In the general scenario where AGN activity marks and regulates the transition from late-type disk galaxies into massive elliptical galaxies, this work suggests that the earlier stages are most evident as mid-IR selected AGNs. Mid-IR emission is less susceptible to absorption than the relatively soft X-rays probed by XMM-Newton, which are seen at later stages in the transition. Radio-selected AGN are then typically associated with minor bursts of activity in the most massive galaxies.Comment: 28 page