Analytical aspects of diterpene alkaloid poisoning with monkshood.

A sensitive and specific method for aconitine extraction from biological samples was developed. Aconitine, the main toxic alkaloid from plants belonging to Aconitum species (family Ranunculaceae), was determined in plant material by an external standard method, and by a standard addition calibration method in biological fluids. Described here is one fatal case and five intoxications of accidental aconitine poisoning following the ingestion of aconite mistaken for an edible grass, Aruncus dioicus (Walt.) Fernald, "mountain asparagus", and Cicerbita alpina (L.) Wallroth. The aconitine content in urine was in the range 2.94 μg/mL (dead patient) – 0.20 μg/mL (surviving patients), which was almost two to four times higher than that in plasma

From Citywide to Neighborhood-Based: Two Decades of Learning, Prioritization, and Strategic Action to Build the Skillman Foundation’s Youth-Development Systems

· This article explores the Skillman Foundation’s shift in its approach to fulfilling its mission to improve the lives of children and youth and to making grants – moving from a traditional grantmaker to a place-based investor and change-maker. · Three aspects of Skillman’s approach have directly shaped the evolution of its youth-development investments: recognizing Detroit’s economic, social, political, and environmental challenges; articulating overarching goals to provide direction and setting priorities for the scope and focus of its programmatic work; and using rapid learning to inform strategic decisions and social-innovation practices designed to tackle deeply entrenched problems. · This article reflects on the foundation’s evolution over two decades of learning, prioritization, and strategic action in its efforts to build and sustain outcome-focused youth-development systems

Scaling in a post-growth era: Learning from Social Agricultural Cooperatives

It has become normative in organization and management studies literature to consider scaling as a synonym for organizational growth. Scaling is typically understood as scaling-up. This article demonstrates that, in the context of post-growth organizations, scaling involves a more complex set of dynamics. Directing scholarly attention to scaling in the context of Italian Social Agricultural Cooperatives (i.e. organizations that hold a different rationale and modus operandi from the capitalist enterprise), this research contributes to the literature on scaling the impact of post-growth organizations by identifying nine different scaling routes: organizational growth (vertical and horizontal); organizational downscaling; impact on policies; multiplication; impact on organizational culture; impact on societal culture; aggregation; and diffusion. This article demonstrates that post-growth scaling: (1) requires the synergistic interaction of different strategies; (2) focuses on impacting societal culture; (3) does not necessarily require organizational growth; and (4) is a relational process, embedded in socio-ecological systems. The typology presented in this article empowers post-growth organizations to become more aware of different available scaling routes, unlocking their transformative potential and supporting the transition towards a post-growth future, in which the goal of economics is the pursuit of human and ecological flourishing

Refining virtual cross-national research collaboration : drivers, affordances and constraints

Publisher Copyright: © 2022, Irina A. Lokhtina, Laura Colombo, Citra Amelia, Erika Löfström, Anu Tammeleht, Anna Sala-Bubare, Marian Jazvac-Martek, Montserrat Castelló and Lynn McAlpine.Purpose: The study aims to explore and explain the affordances and constraints of two-mode virtual collaboration as experienced by a newly forming international research team. Design/methodology/approach: This is self-reflective and action-oriented research on the affordances and constraints of two-mode virtual collaboration. In the spirit of professional development, the authors (nine researchers at different career stages and from various counties) engaged in a joint endeavour to evaluate the affordances and constraints of virtual collaborations in light of the recent literature while also researching the authors' own virtual collaboration during this evaluative task (mid-January–April 2021). The authors used two modes: synchronous (Zoom) and asynchronous (emails) to communicate on the literature exploration and recorded reactions and emotional responses towards existing affordances and constraints through a collective journal. Findings: The results suggest both affordances in terms of communication being negotiable and evolving and constraints, particularly in forming new relations given tools that may not be equally accessible to all. Journaling during collaborations could be a valuable tool, especially for virtual collective work, because it can be used to structure the team supported negotiation and discussion processes, especially often hidden processes. It is evident that the role of a leader can contribute to an alignment in the assumptions and experiences of trust and consequently foster greater mutual understanding of the circumstances for productive team collaborations. Originality/value: The findings of this study can inform academics and practitioners on how to create and facilitate better opportunities for collaboration in virtual teams as a rapidly emerging form of technology-supported working.Peer reviewe

Refining virtual cross-national research collaboration : drivers, affordances and constraints

Publisher Copyright: © 2022, Irina A. Lokhtina, Laura Colombo, Citra Amelia, Erika Löfström, Anu Tammeleht, Anna Sala-Bubare, Marian Jazvac-Martek, Montserrat Castelló and Lynn McAlpine.Purpose: The study aims to explore and explain the affordances and constraints of two-mode virtual collaboration as experienced by a newly forming international research team. Design/methodology/approach: This is self-reflective and action-oriented research on the affordances and constraints of two-mode virtual collaboration. In the spirit of professional development, the authors (nine researchers at different career stages and from various counties) engaged in a joint endeavour to evaluate the affordances and constraints of virtual collaborations in light of the recent literature while also researching the authors' own virtual collaboration during this evaluative task (mid-January–April 2021). The authors used two modes: synchronous (Zoom) and asynchronous (emails) to communicate on the literature exploration and recorded reactions and emotional responses towards existing affordances and constraints through a collective journal. Findings: The results suggest both affordances in terms of communication being negotiable and evolving and constraints, particularly in forming new relations given tools that may not be equally accessible to all. Journaling during collaborations could be a valuable tool, especially for virtual collective work, because it can be used to structure the team supported negotiation and discussion processes, especially often hidden processes. It is evident that the role of a leader can contribute to an alignment in the assumptions and experiences of trust and consequently foster greater mutual understanding of the circumstances for productive team collaborations. Originality/value: The findings of this study can inform academics and practitioners on how to create and facilitate better opportunities for collaboration in virtual teams as a rapidly emerging form of technology-supported working.Peer reviewe

Clinical and genetic characterization of chanarin-dorfman syndrome patients: first report of large deletions in the ABHD5 gene

<p>Abstract</p> <p>Background</p> <p>Chanarin-Dorfman syndrome (CDS) is a rare autosomal recessive disorder characterized by nonbullous congenital ichthyosiform erythroderma (NCIE) and an intracellular accumulation of triacylglycerol (TG) droplets in most tissues. The clinical phenotype involves multiple organs and systems, including liver, eyes, ears, skeletal muscle and central nervous system (CNS). Mutations in ABHD5/CGI58 gene are associated with CDS.</p> <p>Methods</p> <p>Eight CDS patients belonging to six different families from Mediterranean countries were enrolled for genetic study. Molecular analysis of the ABHD5 gene included the sequencing of the 7 coding exons and of the putative 5' regulatory regions, as well as reverse transcript-polymerase chain reaction analysis and sequencing of normal and aberrant ABHD5 cDNAs.</p> <p>Results</p> <p>Five different mutations were identified, four of which were novel, including two splice-site mutations (c.47+1G>A and c.960+5G>A) and two large deletions (c.898_*320del and c.662-1330_773+46del). All the reported mutations are predicted to be pathogenic because they lead to an early stop codon or a frameshift producing a premature termination of translation. While nonsense, missense, frameshift and splice-site mutations have been identified in CDS patients, large genomic deletions have not previously been described.</p> <p>Conclusions</p> <p>These results emphasize the need for an efficient approach for genomic deletion screening to ensure an accurate molecular diagnosis of CDS. Moreover, in spite of intensive molecular screening, no mutations were identified in one patient with a confirmed clinical diagnosis of CDS, appointing to genetic heterogeneity of the syndrome.</p

Correction:Clinical and genetic characterization of chanarin-dorfman syndrome patients: first report of large deletions in the ABHD5 gene

AbstractFollowing the publication of this article [Redaelli C et al, Clinical and genetic characterization of Chanarin-Dorfman Syndrome patients: first report of large deletions in the ABHD5 gene. Orphanet J Rare Dis 2010; 5: 33.], it was clarified that the clinical follow-up of one of CDS family described in the manuscript was performed by Dr. Amalia Sertedaki and Talia Kakourou. The authorship of the article has been changed accordingly. The submitting authors would like to apologise to Amalia Sertedaki and Talia Kakourou for this error and they would like to thank Catherine Dacou-Voutetakis for underlining the problem

Towards a new classification of galaxies: principal component analysis of CALIFA circular velocity curves

We present a galaxy classification system for 238 (E1-Sdm) CALIFA (Calar Alto Legacy Integral Field Area) galaxies based on the shapes and amplitudes of their circular velocity curves (CVCs). We infer the CVCs from the de-projected surface brightness of the galaxies, after scaling by a constant mass-to-light ratio based on stellar dynamics - solving axisymmetric Jeans equations via fitting the second velocity moment $V_{\mathrm{rms}}=\sqrt{V^2+\sigma^2}$ of the stellar kinematics. We use principal component analysis (PCA) applied to the CVC shapes to find characteristic features and use a $k$-means classifier to separate circular curves into classes. This objective classification method identifies four different classes, which we name slow-rising (SR), flat (FL), round-peaked (RP) and sharp-peaked (SP) circular curves. SR are typical for low-mass, late-type (Sb-Sdm), young, faint, metal-poor and disc-dominated galaxies. SP are typical for high-mass, early-type (E1-E7), old, bright, metal-rich and bulge-dominated galaxies. FL and RP appear presented by galaxies with intermediate mass, age, luminosity, metallicity, bulge-to-disk ratio and morphologies (E4-S0a, Sa-Sbc). The discrepancy mass factor, $f_d=1-M_{*}/M_{dyn}$, have the largest value for SR and SP classes ($\sim$ 74 per cent and $\sim$ 71 per cent, respectively) in contrast to the FL and RP classes (with $\sim$ 59 per cent and $\sim$ 61 per cent, respectively). Circular curve classification presents an alternative to typical morphological classification and appears more tightly linked to galaxy evolution.Comment: Accepted for publication in MNRAS (Minor changes), 123 pages, 19 figures, 87 Tables (containing the basic properties of the 238 E1-Sdm galaxies; the five main Principal Component Eigenvectors; the five main Principal Components - PC_i; the Multi-Gaussian Expansion models - MGEs; the circular velocity curve models and their uncertainties

Gerstmann-Sträussler-Scheinker disease amyloid protein polymerizes according to the "dock-and-lock" model.

Prion protein (PrP) amyloid formation is a central feature of genetic and acquired prion diseases such as Gerstmann-Sträussler-Scheinker disease (GSS) and variant Creutzfeldt-Jakob disease. Themajor component of GSS amyloid is a PrP fragment spanning residues ∼82-146, which when synthesized as a peptide, readily forms fibrils featuring GSS amyloid. The present study employed surface plasmon resonance (SPR) to characterize the binding events underlying PrP82-146 oligomerization at the first stages of fibrillization, according to evidence suggesting a pathogenic role of prefibrillar oligomers rather than mature amyloid fibrils. We followed in real time the binding reactions occurring during short term (seconds) addition of PrP82-146 small oligomers (1-5-mers, flowing species) onto soluble prefibrillar PrP82-146 aggregates immobilized on the sensor surface. SPR data confirmed very efficient aggregation/elongation, consistent with the hypothesis of nucleation-dependent polymerization process. Much lower binding was observed when PrP82-146 flowed onto the scrambled sequence of PrP82-146 or onto prefibrillar Aβ42 aggregates. As previously found with Aβ40, SPR data could be adequately fitted by equations modeling the "dock-and-lock" mechanism, in which the "locking" step is due to sequential conformational changes, each increasing the affinity of the monomerfor the fibril until a condition of irreversible binding is reached. However, these conformational changes (i.e. the locking steps) appear to be faster and easier with PrP82-146 than with Aβ40. Such differences suggest that PrP82-146 has a greater propensity to polymerize and greater stability of the aggregates. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc

Ácido ursólico: un compuesto de origen natural con actividad antiviral en infecciones in vitro por rotavirus

Rotavirus (RV) es el principal agente causal de gastroenteritis aguda en niños menores de 5 años. En la Argentina las infecciones por RV son responsables de más del 1,2% de las muertes totales en este grupo etario. A partir de 2015 se ha incorporado una vacuna anti-RV como parte del calendario nacional de vacunación; sin embargo, su eficacia y seguridad continúan siendo evaluadas. El Ácido Ursólico (AU) es un triterpeno que se encuentra formando parte de la estructura de saponinas en diversas plantas. Diversos estudios han demostrado que el AU tiene actividad antiviral frente a ciertos virus. Por ello, nuestro grupo de investigación se centró en la evaluación del posible efecto antiviral del compuesto AU frente a RV, a partir de la utilización de un modelo de infección por RV in vitro, trabajando con la línea celular susceptible MA104. Nuestra hipótesis de trabajo es que el AU podría tener acción antiviral en infecciones por RV, afectando a una o más etapas de su ciclo de replicación. En primer lugar, con el fin de determinar las concentraciones de trabajo no citotóxicas del compuest