Genotypic differences in systemic root responses to mechanical obstacles

As roots grow through the soil to forage for water and nutrients, they encounter mechanical obstacles such as patches of dense soil and stones that locally impede root growth. Here, we investigated hitherto poorly understood systemic responses of roots to localised root impedance. Seedlings of two wheat genotypes were grown in hydroponics and exposed to impenetrable obstacles constraining the vertical growth of the primary or a single seminal root. We deployed high-resolution in vivo imaging to quantify temporal dynamics of root elongation rate, helical root movement, and root growth direction. The two genotypes exhibited distinctly different patterns of systemic responses to localised root impedance, suggesting different strategies to cope with obstacles, namely stress avoidance and stress tolerance. Shallower growth of unconstrained seminal roots and more pronounced helical movement of unconstrained primary and seminal roots upon localised root impedance characterised the avoidance strategy shown by one genotype. Stress tolerance to localised root impedance, as exhibited by the other genotype, was indicated by relatively fast elongation of primary roots and steeper seminal root growth. These different strategies highlight that the effects of mechanical obstacles on spatiotemporal root growth patterns can differ within species, which may have major implications for resource acquisition and whole-plant growth

Genetic Diversity under Soil Compaction in Wheat: Root Number as a Promising Trait for Early Plant Vigor

Soil compaction of arable land, caused by heavy machinery constitutes a major threat to agricultural soils in industrialized countries. The degradation of soil structure due to compaction leads to decreased (macro-) porosity resulting in increased mechanical impedance, which adversely affects root growth and crop productivity. New crop cultivars, with root systems that are adapted to conditions of increased soil strength, are needed to overcome the limiting effects of soil compaction on plant growth. This study aimed (i) to quantify the genetic diversity of early root system development in wheat and to relate this to shoot development under different soil bulk densities and (ii) to test whether root numbers are suitable traits to assess the genotypic tolerance to soil compaction. Fourteen wheat genotypes were grown for 3 weeks in a growth chamber under low (1.3 g cm-3), moderate (1.45 g cm-3), and high soil bulk density (1.6 g cm-3). Using X-ray computed tomography root system development was quantified in weekly intervals, which was complemented by weekly measurements of plant height. The development of the root system, quantified via the number of axial and lateral roots was strongly correlated (0.78 < r < 0.88, p < 0.01) to the development of plant height. Furthermore, significant effects (p < 0.01) of the genotype on root system development and plant vigor traits were observed. Under moderate soil strength final axial and lateral root numbers were significantly correlated (0.57 < r < 0.84, p < 0.05) to shoot dry weight. Furthermore, broad-sense heritability of axial and lateral root number was higher than 50% and comparable to values calculated for shoot traits. Our results showed that there is genetic diversity in wheat with respect to root system responses to increased soil strength and that root numbers are suitable indicators to explain the responses and the tolerance to such conditions. Since root numbers are heritable and can be assessed at high throughput rates under laboratory and field conditions, root number is considered a promising trait for screening toward compaction tolerant varieties.ISSN:1664-462

Beyond growth: The significance of non-growth anabolism for microbial carbon-use efficiency in the light of soil carbon stabilisation

Microbial carbon-use efficiency (CUE) in soils captures carbon (C) partitioning between anabolic biosynthesis of microbial metabolites and catabolic C emissions (i.e. respiratory C waste). The use of C for biosynthesis provides a potential for the accumulation of microbial metabolic residues in soil. Recognised as a crucial control in C cycling, microbial CUE is implemented in the majority of soil C models. Due to the models' high sensitivity to CUE, reliable soil C projections demand accurate CUE quantifications. Current measurements of CUE neglect microbial non-growth metabolites, such as extracellular polymeric substances (EPS) or exoenzymes, although they remain in soil and could be quantitatively important. Here, we highlight that disregarding non-growth anabolism can lead to severe underestimations of CUE. Based on two case studies, we demonstrate that neglecting exoenzyme and EPS production underestimates CUE by more than 100% and up to 30%, respectively. By incorporating these case-specific values in model simulations, we observed that the model projects up to 34% larger SOC stocks over a period of 64 years when non-growth metabolites are considered for estimating CUE, highlighting the crucial importance of accurate CUE quantification. Our considerations outlined here challenge the current ways how CUE is measured and we suggest improvements concerning the quantification of nongrowth metabolites. Research efforts should focus on (i) advancing CUE estimations by capturing the multitude of microbial C uses, (ii) improving techniques to quantify non-growth metabolic products in soil, and (iii) providing an understanding of dynamic metabolic C uses under different environmental conditions and over time. In the light of current discussion on soil C stabilisation mechanisms, we call for efforts to open the 'black box' of microbial physiology in soil and to incorporate all quantitative important C uses in CUE measurements

Developing strategies to recover crop productivity after soil compaction — a plant eco-physiological perspective

Soil compaction constitutes a major threat to the fertility of arable soils and food security. The aim of this paperis to highlight the need and opportunities for plant eco-physiological approaches to identify strategies to recovercrop yields after soil compaction. Reduced productivity on compacted soil primarily results from decreased rootelongation rates and thus limited accessibility to water and nutrients. Hence, strategies to recover productivityafter compaction must address plant eco-physiological phenomena that underlie low root system expansionrates. In compacted soil, root growth is decreased due to high soil penetration resistance and due to low oxygenconcentration in soil air caused by reducedfluid transport capability. Thus, plant roots are exposed to a multi-stress environment, which needs to be addressed directly when aiming to recover productivity after compactionin the long-term. Here, we discuss possibilities to increase root growth in order to enhance resource accessibilityand recover crop productivity on compacted soil. Yield recovery can be achieved through breeding of novelcultivars and targeted soil management approaches. On the one hand, the tolerance of plants to the different soilphysical stresses can be enhanced by selecting for specific root traits that facilitate root growth in compactedsoil. Soil management approaches that improve specific physical properties of compacted soil on the other handcan facilitate root growth in compacted soil. Since plant roots are major drivers of soil structure dynamics,increasing root growth in compacted soil may not only mitigate crop productivity losses but also recover soilstructure

Relationships between weather and yield anomalies vary with crop type and latitude in Sweden

CONTEXT: Information on how crop yields are affected by weather variations and extreme weather is needed to develop climate adaptation measures for arable cropping systems. Here, we analysed the effects of weather anomalies and soil texture on crop yield anomalies across Sweden from 1965 to 2020. OBJECTIVE: The aims of this study were to (i) assess the effects of temperature and precipitation anomalies and extreme weather on crop yield anomalies for major field crops across Sweden, (ii) quantify how crop responses to weather anomalies vary along the north-south climate gradient across Sweden, and (iii) elucidate the impacts of soil texture on yield responses to weather anomalies. METHODS: We used daily mean air temperature, daily total precipitation, soil texture and crop yield data from public databases covering all 21 counties in Sweden. Yield data was detrended to account for the effects of agricultural intensification on crop productivity. To assess seasonal weather influences on crop yields, temporal trends of daily average temperature and daily total precipitation were detrended for each season containing a three-month period. We also used a water balance index and a heat wave index to evaluate the impact of extreme weather. RESULTS AND CONCLUSIONS: Our analyses showed that years with extreme weather during summer (i.e. heat waves, drought or water excess) resulted in the largest negative yield anomalies. Spring-sown crops were more negatively affected by extreme weather compared to autumn-sown crops, which we associate with differences in the lengths of the growth period for autumn- and spring-sown crops. Effects of soil texture on yield anomalies were found for spring-sown cereals, where negative effects of drought were exacerbated with increasing sand content. Moreover, we showed that the effects of weather conditions on crop yield anomalies differed between different regions within the country. In northern Sweden, crop yields were more sensitive to excess water, while drought effects were more pronounced in southern Sweden. Similarly, increased summer temperatures favoured crop yields in northern Sweden but had a negative impact on crop yields in the southern part of the country. SIGNIFICANCE: Our study demonstrates that weather impacts on yields vary between crops and locations, and that adaptation to future climate will require crop- and site-specific strategies

No Till and Organic Farming Improve Soil Properties but Reduce Crop Yield Compared to Conventional Farming in a Swiss Farm Network

Soils are of vital importance for sustainable food production. In order to maintain or improve soil quality, it is necessary to develop strategies for a sustainable use of soil. Alternative cropping practices such as reduced tillage and improved crop rotation are more and more adopted with the aim of decreasing the impact of agriculture on the environment. However, their on-the-ground impact in Swiss farming systems still has to be assessed. In this study, we quantified the impact of three farming systems (conventional farming, no-till, and organic farming) on plant and soil chemical, biological and physical properties. Our study included 20 fields for each farming system. All selected fields were cultivated with winter wheat the year of sampling. Soil was sampled at four layers, 0-5 cm, 5-20 cm, 20-25 cm, 25-50 cm. The main variables analysed were grain yield, soil nutrient availability, organic carbon stocks, bulk density, aggregation, porosity and soil biology. This was complemented with a comprehensive survey to collect information about cropping practices at field and farm scale, including organic matter inputs, fertilisation, tillage, phytosanitary treatments, and crop rotation.Our results show a significant influence of cropping practices on plant and soil properties. Wheat yield in no till and organic systems was reduced by 10% and 30% compared to conventional systems. Bulk density was higher in no-till than in ploughed fields in the 5-20 cm layer but similar in the subsoil. A strong stratification with depth of nutrients and soil organic carbon was observed in no-till fields. No-till and organic fields showed larger soil aggregates and higher microbial biomass in the surface layer (0-5 cm). Mycorrhizal colonisation of wheat roots was on average 50% higher in organic fields. However, no differences in carbon stock in the 0-20 cm layer was observed and the ratio organic matter / clay shows a high variability (from poor to good) and was not dependent on the farming system.Our results show that an improvement of soil properties can be achieved with alternative cropping practices such as no-till and organic farming, but also depends on the other practices adopted by the farmers, such as input of organic amendments, crop rotation diversification, residue management

Evidence for magnesium-phosphorus synergism and co-limitation of grain yield in wheat agriculture

Modern crop production is characterized by high nitrogen (N) application rates, which can influence the co-limitation of harvested yield by other nutrients. Using a multidimensional niche volume concept and scaling exponents frequently applied in plant ecological research, we report that increased N and phosphorus (P) uptake in a growing wheat crop along with enhanced grain biomass is associated with more than proportional increase of other nutrients. Furthermore, N conversion efficiency and grain yield are strongly affected by the magnesium (Mg) to P ratio in the growing crop. We analyzed a field trial in Central Sweden including nine wheat varieties grown during two years with contrasting weather, and found evidence for Mg co-limitation at lower grain yields and P co-limitation at higher yields. We argue that critical concentrations of single nutrients, which are often applied in agronomy, should be replaced by nutrient ratios. In addition, links between plant P and Mg contents and root traits were found; high root number enhanced the P:N ratio, whilst steep root angle, indicating deep roots, increased the Mg:N ratio. The results have significant implications on the management and breeding targets of agriculturally grown wheat, which is one of the most important food crops worldwide

Limited resilience of the soil microbiome to mechanical compaction within four growing seasons of agricultural management

Soil compaction affects many soil functions, but we have little information on the resistance and resilience of soil microorganisms to this disturbance. Here, we present data on the response of soil microbial diversity to a single compaction event and its temporal evolution under different agricultural management systems during four growing seasons. Crop yield was reduced (up to −90%) in the first two seasons after compaction, but mostly recovered in subsequent seasons. Soil compaction increased soil bulk density (+15%), and decreased air permeability (−94%) and gas diffusion (−59%), and those properties did not fully recover within four growing seasons. Soil compaction induced cropping system-dependent shifts in microbial community structures with little resilience over the four growing seasons. Microbial taxa sensitive to soil compaction were detected in all major phyla. Overall, anaerobic prokaryotes and saprotrophic fungi increased in compacted soils, whereas aerobic prokaryotes and plant-associated fungi were mostly negatively affected. Most measured properties showed large spatial variability across the replicated blocks, demonstrating the dependence of compaction effects on initial conditions. This study demonstrates that soil compaction is a disturbance that can have long-lasting effects on soil properties and soil microorganisms, but those effects are not necessarily aligned with changes in crop yield

A time-lapse imaging platform for quantification of soil crack development due to simulated root water uptake

Plants are major drivers of soil structure dynamics. Root growth creates new macropores and provides essential carbon to soil, while root water uptake may induce crack formation around roots. Cracks can facilitate root growth as they provide pathways of least resistance and improve water infiltration and soil aeration. Due to the lack of suitable quantification methods, knowledge on the effects of root water uptake on soil crack formation remains limited. In the current study, we developed a time-lapse imaging platform that allows i) simulating root water uptake through localized soil drying and ii) quantifying the development of two-dimensional crack networks. Customized soil boxes that were 50 mm wide, 55 mm high and 5 mm deep were designed. Artificial roots made of dialysis tubes were inserted into the soil boxes and polyethylene glycol solution was circulated through the tubes. This induced a gradient in osmotic potential at the contact area (150 mm(2)) between the soil and the dialysis tubes, resulting in controlled soil drying. Drying intensity was varied by using different polyethylene glycol concentrations. Experiments were conducted with three soils that were subjected to three drying intensities for 6.5 days. We developed a time-lapse imaging system to record soil crack formation at two-minute intervals in twelve samples simultaneously. Resulting crack networks were quantified with an automated image analysis pipeline. Across soils and drying intensities, crack network development slowed down after 24-48 h of soil drying. The extent and complexity of crack networks increased with drying intensity and crack networks were larger and more complex in the clay and clay loam soil than in the silt loam soil. Smaller and less complex crack networks were better connected than larger and more complex networks. These results demonstrate that the platform developed in this study is suitable to quantify crack network development in soil due to simulated root water uptake at high temporal resolution and high throughput. Thereby, it can provide information needed to improve our understanding on how plants modify soil structure