Complex behavioural changes after odour exposure in Drosophila larvae

A variety of odorants attract Drosophila larvae, although this behaviour can be modulated by experience. For instance, larvae pre-exposed to an attractive odorant may subsequently display less attraction towards the same compound. In previous reports, this phenomenon has been interpreted as a drop in olfactory sensitivity, caused by sensory adaptation. We tried to elucidate the basis of this behavioural modification by pre-exposing larvae to various odours. After multiple pre-exposure cycles larvae were repulsed by initially attractive odours, and pre-exposure did not change the threshold concentration driving a behavioural response. We therefore believe that sensitivity to the odorant was only slightly affected in our protocol. Our results thus do not support the previous interpretation and rather suggest that olfactory pre-exposure induces a change in the hedonic value of the odour. Although we did not succeed in elucidating the exact nature of the underlying mechanism, we can reject an association of the odour with the absence of food as an interpretation of the observed behavioural changes; this is because addition of food did not abolish the repulsion to the pre-exposed odour. In addition to ruling out previous interpretations of odour pre-exposure effects, this study stresses the complexity of Drosophila larval behaviour

Energy analysis of organic crop systems. Impact of intensification level.

This study reveals a wide variability in the major parameters of the energy analysis applied to 44 four-year-long organic crop sequences. The intensification level of the systems in terms of the use or nonuse of organic fertilisation on cereals and of irrigation on summer crops (mainly pulses) is largely responsible for this variability. Average energy consumption ranges from 5 000 to 12 270 MJ/ha/year depending on the intensification level. Energy production varies from 35 500 to 43 950 MJ/ha/year. Since energy consumption and production both vary in the same direction with the degree of intensification, the energy gain is stable at an average value of 29300 MJ/ha/year. Energy efficiency decreases from 7.1 MJ/MJ for unfertilised and unirrigated crop sequences, to 3.5 MJ/MJ for fertilised and irrigated sequences, where soy and faba bean are among the major crops

Galectin-3 interacts with the cell surface glycoprotein CD146 (MCAM, MUC18) and induces secretion of metastasis-promoting cytokines from vascular endothelial cells

The galactoside-binding protein galectin-3 is increasingly recognized as an important player in cancer development, progression, and metastasis via its interactions with various galactoside-terminated glycans. We have shown previously that circulating galectin-3, which is increased up to 30-fold in cancer patients, promotes blood-borne metastasis in an animal cancer model. This effect is partly attributable to the interaction of galectin-3 with unknown receptor(s) on vascular endothelial cells and causes endothelial secretion of several metastasis-promoting cytokines. Here we sought to identify the galectin-3-binding molecule(s) on the endothelial cell surface responsible for the galectin-3-mediated cytokine secretion. Using two different galectin-3 affinity purification processes, we extracted four cell membrane glycoproteins, CD146/melanoma cell adhesion molecule (MCAM)/MUC18, CD31/platelet endothelial cell adhesion molecule-1 (PECAM-1), CD144/VE-cadherin, and CD106/Endoglin, from vascular endothelial cells. CD146 was the major galectin-3-binding ligand and strongly co-localized with galectin-3 on endothelial cell surfaces treated with exogenous galectin-3. Moreover, galectin-3 bound to N-linked glycans on CD146 and induced CD146 dimerization and subsequent activation of AKT signaling. siRNA-mediated suppression of CD146 expression completely abolished the galectin-3-induced secretion of IL-6 and G-CSF cytokines from the endothelial cells. Thus, CD146/MCAM is the functional galectin-3-binding ligand on endothelial cell surfaces responsible for galectin-3-induced secretion of metastasis-promoting cytokines. We conclude that CD146/MCAM interactions with circulating galectin-3 may have an important influence on cancer progression and metastasis

Gobernanza urbana y retos de la regulación del alquiler vacacional y de las plataformas digitales en las grandes ciudades europeas

El desarrollo exponencial del alquiler vacacional, o de corto plazo, facilitado por plataformas digitales como Airbnb, se ha convertido en los últimos años en uno de los temas más polémicos en las grandes ciudades europeas. A partir de un estudio sobre la regulación del alquiler vacacional en una decena de grandes ciudades europeas, se analizan los procesos de politización de este fenómeno, las medidas de regulación y de control que se han impulsado, su aplicación y la difícil gobernanza de las nuevas plataformas de la economía digital. En el contexto europeo, las respuestas de los gobiernos municipales han sido muy diversas. Algunos han tomado fuertes medidas regulatorias para prohibir o frenar el crecimiento del alquiler vacacional. Otros han tratado de buscar un punto medio entre la protección del uso residencial, la atracción turística de la ciudad y el “derecho a compartir” una residencia principal. Algunas ciudades han optado por una regulación mínima, y otras ciudades todavía no han adoptado medidas específicas. Esta diversidad de modos de regulación se puede explicar por tres factores (Aguilera et al., 2019): el tipo de actores que politizaron el tema y lo enmarcaron dentro de un sector de políticas públicas específico; los instrumentos preexistentes utilizados en ese sector; y la distribución de competencias entre el nivel municipal y niveles de gobierno de rango superior. Sin embargo, la aplicación de las nuevas regulaciones permanece inacabada e inefectiva, en gran parte debido a la falta de cooperación de las plataformas digitales en facilitar el acceso a los datos de los anunciantes. La efectividad futura de la regulación depende del éxito de las presiones puestas por los actores públicos sobre las plataformas para conseguir el acceso a los datos y el cumplimiento normativo, una batalla que en Europa se juega ahora a nivel de las instituciones de la UE

GHRS and ORFEUS-II Observations of the Highly Ionized Interstellar Medium Toward ESO141-055

We present Goddard High Resolution Spectrograph and ORFEUS-II measurements of Si IV, CIV, N V, and O VI absorption in the interstellar medium of the Galactic disk and halo toward the nucleus of the Seyfert galaxy ESO141-055. The high ionization absorption is strong, with line strengths consistent with the spectral signature expected for hot (log T = 5-6) collisionally ionized gas in either a ``Galactic fountain'' or an inhomogeneous medium containing a mixture of conductive interfaces and turbulent mixing layers. The total O VI column density of log N ~ 15 suggests that the scale height of O VI is large (>3 kpc) in this direction. Comparison of the high ion column densities with measurements for other sight lines indicates that the highly ionized gas distribution is patchy. The amount of O VI perpendicular to the Galactic plane varies by at least a factor of ~4 among the complete halo sight lines thus far studied. In addition to the high ion absorption, lines of low ionization species are also present in the spectra. With the possible exception of Ar I, which may have a lower than expected abundance resulting from partial photoionization of gas along the sight line, the absorption strengths are typical of those expected for the warm, neutral interstellar medium. The sight line intercepts a cold molecular cloud with log N(H2) ~ 19. The cloud has an identifiable counterpart in IRAS 100-micron emission maps of this region of the sky. We detect a Ly-alpha absorber associated with ESO141-055 at z = 0.03492. This study presents an enticing glimpse into the interstellar and intergalactic absorption patterns that will be observed at high spectral resolution by the Far Ultraviolet Spectroscopic Explorer.Comment: 24 pages + 8 figures, uses aaspp4.sty. Accepted for publication in Ap

A radio continuum survey of the southern sky at 1420 MHz. Observations and data reduction

We describe the equipment, observational method and reduction procedure of an absolutely calibrated radio continuum survey of the South Celestial Hemisphere at a frequency of 1420 MHz. These observations cover the area 0h < R.A. < 24h for declinations less than -10 degree. The sensitivity is about 50 mK T_B (full beam brightness) and the angular resolution (HPBW) is 35.4', which matches the existing northern sky survey at the same frequency.Comment: 9 pages with 9 figures, A&A, in pres

Imaging Gold Nanoparticles in Living Cells Environments using Heterodyne Digital Holographic Microscopy

This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environment. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40 nm gold nanoparticles, and the biological specimen is imaged in a total internal reflection configuration with holographic microscopy. Due to a higher scattering efficiency of the gold nanoparticles versus that of cellular structures, accurate localization of a gold marker is obtained within a 3D mapping of the entire sample's scattered field, with a lateral precision of 5 nm and 100 nm in the x,y and in the z directions respectively, demonstrating the ability of holographic microscopy to locate nanoparticles in living cells environments

Advancing national greenhouse gas inventories for agriculture in developing countries : improving activity data, emission factors and software technology

Peer reviewedPublisher PD

Seasonal variations in aerosol particle composition at the puy-de-Dôme research station in France

Detailed investigations of the chemical and microphysical properties of atmospheric aerosol particles were performed at the puy-de-Dôme (pdD) research station (1465 m) in autumn (September and October 2008), winter (February and March 2009), and summer (June 2010) using a compact Time-of-Flight Aerosol Mass Spectrometer (cToF-AMS). Over the three campaigns, the average mass concentrations of the non-refractory submicron particles ranged from 10 μg m&lt;sup&gt;&amp;minus;3&lt;/sup&gt; up to 27 μg m&lt;sup&gt;&amp;minus;3&lt;/sup&gt;. Highest nitrate and ammonium mass concentrations were measured during the winter and during periods when marine modified airmasses were arriving at the site, whereas highest concentrations of organic particles were measured during the summer and during periods when continental airmasses arrived at the site. The measurements reported in this paper show that atmospheric particle composition is strongly influenced by both the season and the origin of the airmass. The total organic mass spectra were analysed using positive matrix factorisation to separate individual organic components contributing to the overall organic particle mass concentrations. These organic components include a low volatility oxygenated organic aerosol particle (LV-OOA) and a semi-volatile organic aerosol particle (SV-OOA). Correlations of the LV-OOA components with fragments of &lt;i&gt;m/z&lt;/i&gt; 60 and &lt;i&gt;m/z&lt;/i&gt; 73 (mass spectral markers of wood burning) during the winter campaign suggest that wintertime LV-OOA are related to aged biomass burning emissions, whereas organic aerosol particles measured during the summer are likely linked to biogenic sources. Equivalent potential temperature calculations, gas-phase, and LIDAR measurements define whether the research site is in the planetary boundary layer (PBL) or in the free troposphere (FT)/residual layer (RL). We observe that SV-OOA and nitrate particles are associated with air masses arriving from the PBL where as particle composition measured from RL/FT airmasses contain high mass fractions of sulphate and LV-OOA. This study provides unique insights into the effects of season and airmass variability on regional aerosol particles measured at an elevated site