Genome adaptation to chemical stress: clues from comparative transcriptomics in Saccharomyces cerevisiae and Candida glabrata

Comparative transcriptomics of Saccharomyces cerevisiae and Candida glabrata revealed a remarkable conservation of response to drug-induced stress, despite underlying differences in the regulatory networks

Jean Kuntzmann ou les voyages extraordinaires au pays des mathématiques inconnues

bulletin de l'APMEP et non APMED comme incorrectement orthographié dans la base de données HALNational audienceHommage à Jean Kuntzman

Toward Defining the Threshold Between Low and High Glucose Variability in Diabetes

International audienceOBJECTIVE:To define the threshold for excess glucose variability (GV), one of the main features of dysglycemia in diabetes.RESEARCH DESIGN AND METHODS:A total of 376 persons with diabetes investigated at the University Hospital of Montpellier (Montpellier, France) underwent continuous glucose monitoring. Participants with type 2 diabetes were divided into several groups-groups 1, 2a, 2b, and 3 (n = 82, 28, 65, and 79, respectively)-according to treatment: 1) diet and/or insulin sensitizers alone; 2) oral therapy including an insulinotropic agent, dipeptidyl peptidase 4 inhibitors (group 2a) or sulfonylureas (group 2b); or 3) insulin. Group 4 included 122 persons with type 1 diabetes. Percentage coefficient of variation for glucose (%CV = [(SD of glucose)/(mean glucose)] × 100) and frequencies of hypoglycemia (interstitial glucose 36% were compared with those with %CV ≤36%.CONCLUSIONS:A %CV of 36% appears to be a suitable threshold to distinguish between stable and unstable glycemia in diabetes because beyond this limit, the frequency of hypoglycemia is significantly increased, especially in insulin-treated subjects

Actes du Congrès Collèges célébrations 92

Également disponible en version papierTitre de l'écran-titre (visionné le 12 août 2009)Notes (part. bibliogr.): p.

Simulation of an experimental fire in an underground limestone quarry for the study of Paleolithic fires

International audienceNumerous fire marks occur on the walls of the Chauvet-Pont d’Arc cave. Dating indicated that some of the fires were contemporary to the Aurignacian. Violent thermal shocks were observed in surprisingly narrow areas of the cave. This raises numerous archaeological questions about the function of the fires; the answers depend on the location of the hearths, and the intensity of the fires. Numerical simulation was used here to provide information about the behaviour of fires in such confined spaces. An underground non-archaeological site, in a limestone quarry, was equipped to monitor fires in an environment similar to that of the Megaceros gallery of the Chauvet-Pont d’Arc cave. The fire and the movement of heat and smoke in the quarry were simulated by the open source code “Fire Dynamics Simulator (FDS)”. Results were validated on wall temperatures recorded behind and above the fire. The thermo-mechanical impact of the fire on the rock was simulated with CAST3M software, providing the most probable zones for limestone spalling due to thermal gradients. The validated approach will, in a forthcoming study, be applied to the Chauvet-Pont d’Arc cave, in which coupled simulations in the air and in the rock should indicate the location of the hearths and the intensity of the fires that generated the marks

Un outil pour connaître de minute en minute l’expérience d’un visiteur adulte

A description of an instrument to assess the adult visitor’s experience as he lives it. It could reveal not only what he learns, but also the other benefits that he derives from strolling through an exhibition. For instance, it enables to know in details what the visitor feels and imagines, or to study phenomena like fascination and immersion. Description d’un instrument qui permet d’accéder à l’expérience d’un visiteur adulte à mesure qu’il la vit.  Cet instrument peut révéler non seulement ce qu’un visiteur apprend, mais également les autres bénéfices qu’il retire de son passage dans des salles d’exposition.  Il permet aussi, par exemple, d’étudier en détail ce qu’un visiteur ressent et imagine, ou des phénomènes importants comme sa fascination ou son immersion dans un environnement  muséalA description of an instrument to assess the adult visitor’s experience as he lives it. It could reveal not only what he learns, but also the other benefits that he derives from strolling through an exhibition. For instance, it enables to know in details what the visitor feels and imagines, or to study phenomena like fascination and immersion.&nbsp

Cholesterol, Bile Acid, And Lipoprotein Metabolism In Two Strains Of Hamster, One Resistant, The Other Sensitive (LPN) To Sucrose-Induced Cholelithiasis

A comprehensive study of cholesterol, bile acid, and lipoprotein metabolism was undertaken in two strains of hamster that differed markedly in their response to a sucrose-rich/low fat diet. Under basal conditions, hamsters from the LPN strain differed from Janvier hamsters by a lower cholesterolemia, a higher postprandial insulinemia, a more active cholesterogenesis in both liver [3- to 4-fold higher 3-hydroxy 3-methylglutaryl coenzyme A reductase (HMG-CoAR) activity and mRNA] and small intestine, and a lower hepatic acyl-coenzyme A:cholesterol acyltransferase activity. Cholesterol saturation indices in the gallbladder bile were similar for both strains, but the lipid concentration was 2-fold higher in LPN than in Janvier hamsters. LPN hamsters had a lower capacity to transform cholesterol into bile acids, shown by the smaller fraction of endogenous cholesterol converted into bile acids prior to fecal excretion (0.34 vs. 0.77). In LPN hamsters, the activities of cholesterol 7 -hydroxylase (C7OHase) and sterol 27-hydroxylase (S27OHase), the two rate-limiting enzymes of bile acid synthesis, were disproportionably lower (by 2-fold) to that of HMG-CoAR. When fed a sucrose-rich diet, plasma lipids increased, dietary cholesterol absorption improved, hepatic activities of HMG-CoA reductase, C7Ohase, and S27OHase were reduced, and intestinal S27OHase was inhibited in both strains. Despite a similar increase in the biliary hydrophobicity index due to the bile acid enrichment in chenodeoxycholic acid and derivatives, only LPN hamsters had an increased lithogenic index and developed cholesterol gallstones (75% incidence), whereas Janvier hamsters formed pigment gallstones (79% incidence). These studies indicate that LPN hamsters have a genetic predisposition to sucrose-induced cholesterol gallstone formation related to differences in cholesterol and bile acid metabolism