Public Bikesharing in North America During a Period of Rapid Expansion: Understanding Business Models, Industry Trends & User Impacts, MTI Report 12-29

Public bikesharing—the shared use of a bicycle fleet—is an innovative transportation strategy that has recently emerged in major cities around the world, including North America. Information technology (IT)-based bikesharing systems typically position bicycles throughout an urban environment, among a network of docking stations, for immediate access. Trips can be one-way, round-trip, or both, depending on the operator. Bikesharing can serve as a first-and-last mile connector to other modes, as well as for both short and long distance destinations. In 2012, 22 IT-based public bikesharing systems were operating in the United States, with a total of 884,442 users and 7,549 bicycles. Four IT-based programs in Canada had a total of 197,419 users and 6,115 bicycles. Two IT-based programs in Mexico had a total of 71,611 users and 3,680 bicycles. (Membership numbers reflect the total number of short- and long-term users.) This study evaluates public bikesharing in North America, reviewing the change in travel behavior exhibited by members of different programs in the context of their business models and operational environment. This Phase II research builds on data collected during our Phase I research conducted in 2012. During the 2012 research (Phase I), researchers conducted 14 expert interviews with industry experts and public officials in the United States and Canada, as well as 19 interviews with the manager and/or key staff of IT-based bikesharing organizations. For more information on the Phase I research, please see the Shaheen et al., 2012 report Public Bikesharing in North America: Early Operator and User Understanding. For this Phase II study, an additional 23 interviews were conducted with IT-based bikesharing organizations in the United States, Canada, and Mexico in Spring 2013. Notable developments during this period include the ongoing expansion of public bikesharing in North America, including the recent launches of multiple large bikesharing programs in the United States (i.e., Citi Bike in New York City, Divvy in Chicago, and Bay Area Bike Share in the San Francisco Bay Area). In addition to expert interviews, the authors conducted two kinds of surveys with bikesharing users. One was the online member survey. This survey was sent to all people for whom the operator had an email address.The population of this survey was mainly annual members of the bikesharing system, and the members took the survey via a URL link sent to them from the operator. The second survey was an on-street survey. This survey was designed for anyone, including casual users (i.e., those who are not members of the system and use it on a short-term basis), to take “on-street” via a smartphone. The member survey was deployed in five cities: Montreal, Toronto, Salt Lake City, Minneapolis-Saint Paul, and Mexico City. The on-street survey was implemented in three cities: Boston, Salt Lake City, and San Antonio

Noncovariant gauge fixing in the quantum Dirac field theory of atoms and molecules

Starting from the Weyl gauge formulation of quantum electrodynamics (QED), the formalism of quantum-mechanical gauge fixing is extended using techniques from nonrelativistic QED. This involves expressing the redundant gauge degrees of freedom through an arbitrary functional of the gauge-invariant transverse degrees of freedom. Particular choices of functional can be made to yield the Coulomb gauge and Poincar\'{e} gauge representations. The Hamiltonian we derive therefore serves as a good starting point for the description of atoms and molecules by means of a relativistic Dirac field. We discuss important implications for the ontology of noncovariant canonical QED due to the gauge freedom that remains present in our formulation.Comment: 8 pages, 0 figure

Simultaneous mapping of membrane voltage and calcium in zebrafish heart in vivo reveals chamber-specific developmental transitions in ionic currents

The cardiac action potential (AP) and the consequent cytosolic Ca2+ transient are key indicators of cardiac function. Natural developmental processes, as well as many drugs and pathologies change the waveform, propagation, or variability (between cells or over time) of these parameters. Here we apply a genetically encoded dual-function calcium and voltage reporter (CaViar) to study the development of the zebrafish heart in vivo between 1.5 and 4 days post fertilization (dpf). We developed a high-sensitivity spinning disk confocal microscope and associated software for simultaneous three-dimensional optical mapping of voltage and calcium. We produced a transgenic zebrafish line expressing CaViar under control of the heart-specific cmlc2 promoter, and applied ion channel blockers at a series of developmental stages to map the maturation of the action potential in vivo. Early in development, the AP initiated via a calcium current through L-type calcium channels. Between 90 and 102 h post fertilization (hpf), the ventricular AP switched to a sodium-driven upswing, while the atrial AP remained calcium driven. In the adult zebrafish heart, a sodium current drives the AP in both the atrium and ventricle. Simultaneous voltage and calcium imaging with genetically encoded reporters provides a new approach for monitoring cardiac development, and the effects of drugs on cardiac function

Genetic recombination is targeted towards gene promoter regions in dogs

The identification of the H3K4 trimethylase, PRDM9, as the gene responsible for recombination hotspot localization has provided considerable insight into the mechanisms by which recombination is initiated in mammals. However, uniquely amongst mammals, canids appear to lack a functional version of PRDM9 and may therefore provide a model for understanding recombination that occurs in the absence of PRDM9, and thus how PRDM9 functions to shape the recombination landscape. We have constructed a fine-scale genetic map from patterns of linkage disequilibrium assessed using high-throughput sequence data from 51 free-ranging dogs, Canis lupus familiaris. While broad-scale properties of recombination appear similar to other mammalian species, our fine-scale estimates indicate that canine highly elevated recombination rates are observed in the vicinity of CpG rich regions including gene promoter regions, but show little association with H3K4 trimethylation marks identified in spermatocytes. By comparison to genomic data from the Andean fox, Lycalopex culpaeus, we show that biased gene conversion is a plausible mechanism by which the high CpG content of the dog genome could have occurred.Comment: Updated version, with significant revision

Afriflu2—Second international workshop on influenza vaccination in the African continent—8 November 2012, Cape Town (South Africa)

AbstractThe second meeting of the Afriflu conferences took place in Cape Town, South Africa, with over 60 participants from 15 countries in Africa and also outside the continent. Significant progress in surveillance has been made in better understanding the illness burden of influenza on the continent, which limited evidence suggests is greater than that in the developed world. In southern Africa HIV and TB coinfections play a major role in increasing hospitalisation and mortality, while elsewhere in Africa other cofactors still need to be determined.There is currently no indigenous vaccine production in sub-Saharan Africa and only one facility, based in South Africa, capable of filling imported bulk. Innovative vaccine strategies will need to be explored, such as maternal immunisation, and also the possibility of other influenza vaccine options, such as live attenuated influenza vaccine for young children. Sustained indigenous vaccine production is essential for the continent to have vaccine security in the event of a pandemic even though establishing local production faces considerable challenges especially ensuring adequate markets on the continent. There is an urgent need to develop effective communication messages for decision makers as well as healthcare workers addressing the importance of influenza even in the face of the major competing health burdens of the continent

Bright and Fast Multicoloured Voltage Reporters via Electrochromic FRET

Genetically encoded fluorescent reporters of membrane potential promise to reveal aspects of neural function not detectable by other means. We present a palette of multicoloured brightly fluorescent genetically encoded voltage indicators with sensitivities from 8–13% ΔF/F per 100 mV, and half-maximal response times from 4–7 ms. A fluorescent protein is fused to an archaerhodopsin-derived voltage sensor. Voltage-induced shifts in the absorption spectrum of the rhodopsin lead to voltage-dependent nonradiative quenching of the appended fluorescent protein. Through a library screen, we identify linkers and fluorescent protein combinations that report neuronal action potentials in cultured rat hippocampal neurons with a single-trial signal-to-noise ratio from 7 to 9 in a 1 kHz imaging bandwidth at modest illumination intensity. The freedom to choose a voltage indicator from an array of colours facilitates multicolour voltage imaging, as well as combination with other optical reporters and optogenetic actuators.Chemistry and Chemical BiologyEngineering and Applied SciencesPhysic

Using Synchronization for Prediction of High-Dimensional Chaotic Dynamics

We experimentally observe the nonlinear dynamics of an optoelectronic time-delayed feedback loop designed for chaotic communication using commercial fiber optic links, and we simulate the system using delay differential equations. We show that synchronization of a numerical model to experimental measurements provides a new way to assimilate data and forecast the future of this time-delayed high-dimensional system. For this system, which has a feedback time delay of 22 ns, we show that one can predict the time series for up to several delay periods, when the dynamics is about 15 dimensional.Comment: 10 pages, 4 figure

A Smirnov-Bickel-Rosenblatt theorem for compactly-supported wavelets

In nonparametric statistical problems, we wish to find an estimator of an unknown function f. We can split its error into bias and variance terms; Smirnov, Bickel and Rosenblatt have shown that, for a histogram or kernel estimate, the supremum norm of the variance term is asymptotically distributed as a Gumbel random variable. In the following, we prove a version of this result for estimators using compactly-supported wavelets, a popular tool in nonparametric statistics. Our result relies on an assumption on the nature of the wavelet, which must be verified by provably-good numerical approximations. We verify our assumption for Daubechies wavelets and symlets, with N = 6, ..., 20 vanishing moments; larger values of N, and other wavelet bases, are easily checked, and we conjecture that our assumption holds also in those cases