NGS coverage and relative abundance of taxonomically assigned sequence reads obtained from koalas and ticks (<i>Ixodes holocyclus</i> and <i>Ixodes tasmani</i>).

<p>NGS coverage and relative abundance of taxonomically assigned sequence reads obtained from koalas and ticks (<i>Ixodes holocyclus</i> and <i>Ixodes tasmani</i>).</p

Increased genetic diversity and prevalence of co-infection with <i>Trypanosoma</i> spp. in koalas (<i>Phascolarctos cinereus</i>) and their ticks identified using next-generation sequencing (NGS) - Fig 4

<p>Characterisation of mixed trypanosome infections in koalas (A), <i>Ixodes holocyclus</i> (B) and <i>Ixodes tasmani</i> (C), based on the number of unique trypanosome species involved. Each doughnut chart shows proportions of the total number (n) of samples harbouring more than one <i>Trypanosoma</i> sp., based on the NGS analysis.</p

Comparison of prevalence estimates of <i>Trypanosoma</i> spp. obtained using NGS and Sanger sequencing in koalas and ticks (<i>Ixodes holocyclus</i> and <i>Ixodes tasmani</i>).

<p>Comparison of prevalence estimates of <i>Trypanosoma</i> spp. obtained using NGS and Sanger sequencing in koalas and ticks (<i>Ixodes holocyclus</i> and <i>Ixodes tasmani</i>).</p

Prevalence of <i>Trypanosoma</i> spp. detected in koalas, <i>Ixodes holocyclus</i> and <i>Ixodes tasmani</i> ticks, using the NGS method.

<p>Error bars represent 95% confidence intervals (95% CI).</p

Prevalence of single and mixed trypanosome infections in koalas, <i>Ixodes holocyclus</i> and <i>Ixodes tasmani</i>, determined by NGS.

<p>Error bars represent 95% confidence intervals (95% CI).</p

Prevalence of known genotypes of <i>T</i>. <i>copemani</i> and <i>T</i>. <i>vegrandis</i> in koalas and ticks (<i>Ixodes holocyclus</i> and <i>Ixodes tasmani)</i>, determined by NGS at the 18S rRNA locus.

<p>Prevalence of known genotypes of <i>T</i>. <i>copemani</i> and <i>T</i>. <i>vegrandis</i> in koalas and ticks (<i>Ixodes holocyclus</i> and <i>Ixodes tasmani)</i>, determined by NGS at the 18S rRNA locus.</p