Lysine requirements of pre-lay broiler breeder pullets: Determination by indicator amino acid oxidation

The indicator amino acid oxidation (IAAO) method allows the determination of amino acid requirements under conditions of low growth rate as found in pre-laying broiler breeder pullets. Cobb 500 breeder pullets (20 wk old; 2290 +/- 280 g, n = 4) were adapted (6 d) to a pelleted, purified control diet containing all nutrients at greater than or equal to 110% of NRC recommendations. After recovery from surgery for implantation of a jugular catheter, each bird was fed, in random order, test diets containing one of nine levels of lysine (0.48, 0.96, 1.92, 2.88, 3.84, 4.80, 7.68, 9.60 and 14.40 g/kg of diet). Indicator oxidation was determined during 4-h primed (74 kBq/kg body), constant infusions (44 kBq (.) h(-1) (.) kg body(-1)) of L-[1-C-14]phenylalanine. Using the breakpoint of a one-slope broken-line model, the lysine requirement was determined to be 4.88 +/- 0.96 g/kg of diet or 366 +/- 72 mg (.) hen(-1) (.) d(-1) with an upper 95% Cl of 6.40 g/kg of diet or 480 mg (.) hen(-1) (.) d(-1). IAAO allows determination of individual bird amino acid requirements for specific ages and types of birds over short periods of time and enables more accurate broiler breeder pullet diet formulation

Free availability of high-energy foods led to energy over-ingestion and protein under-ingestion in choice-fed broilers

The objective of this study was to compare energy and protein content of the diet selected by choice-fed broilers with thatof broilers fed a balanced diet. One hundred and eighty 1-day-old male broilers were randomly assigned in groups of 10to one of three experimental treatments (n = 6). Control broilers were fed a standard balanced diet, whereas choice-fedbroilers were fed three foods which were more concentrated (Choice C+ treatment) or less concentrated (Choice C?treatment) in protein, carbohydrate or fat. We evaluated food intake behavior, nutrient intake, and performance param-eters of broilers from 2 to 7 weeks of age. Choice C+ broilers showed enhanced preference for the high-fat food, whichled to higher energy intake and lower protein intake than those of control broilers at 2 to 4 weeks of age. Body weight,weight gain and feed conversion efficiency were negatively affected by diet selection of Choice C+ broilers. Choice C?broilers selected a balanced diet, and showed performance parameters similar to those of control broilers. Our results supported the hypothesis that free availability of high-energy foods bias ingestive behavior of choice-fed broilers toward selecting a diet with higher energy and lower protein than needed for normal growth.Fil: Catanese, Francisco Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Rodríguez Ganduglia, Héctor. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Villalba, Juan Jose. State University Of Utah; Estados UnidosFil: Distel, Roberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentin