6 research outputs found
Anti-hyperglycemic effects of aqueous Lenzites betulina extracts from the Philippines on the blood glucose levels of the ICR mice (Mus musculus)
AbstractObjectiveTo examine the anti-hyperglycemic effects of aqueous Lenzites betulina (L. betulina) extracts on normoglycemic glucose-loaded mice.MethodsDifferent doses of aqueous extract from L. betulina were administered to 45 ICR mice (Mus musculus) to determine whether there was an effect of L. betulina extracts on the blood glucose level of the ICR mice. Aqueous extracts of L. betulina were orally gavaged to mice using oral glucose tolerance test. A total of five groups were used to determine the effect of the fungi on blood glucose of the mice. Group A (positive control) was given 16.7 μg/kg glimepiride; Group B (negative control) was given distilled water; Group C (low dosage) was given 200 mg/kg aqueous extract; Group D (mid dosage) was given 400 mg/kg aqueous extract and Group E (high dosage) was given 800 mg/kg aqueous extract. Baseline blood glucose value was firstly acquired before induction of hyperglycemia through d-glucose, after which another check on blood glucose was made after 0.5 h. Immediately, after the acquisition of hyperglycemic blood glucose level, the individual administration of treatments were done. After that, three blood collections were done spanning 3 h with 1 h interval.ResultsThe low dose (200 mg/kg) and the mid dose (400 mg/kg) of L. betulina extracts were significantly different (P < 0.05) from their respective baseline values throughout the whole experiment with the latter surpassing its baseline value during the 3rd hour. On the other hand, the high dose (800 mg/kg) during the 1st hour after administration was not significantly different (P > 0.05) from its corresponding baseline value, acting faster than the positive control (glimepiride), which only became significantly different (P < 0.05) at the 2nd hour.ConclusionsAqueous L. betulina extract is able to produce hypoglycemic effects on the mice with all doses, which are able to normalize blood glucose levels at varying times
Isolation and characterization of hypoglycemic andanticancer glycans from Daedalea quercina (L) Fr.
Hypoglycemic and anticancer glycans were extracted and fractionated from the fruiting bodies of wild mushroom Daedalea quercina (L) Fr. These were obtained from sequential extraction with hot water, 1% Ammonium oxalate and 5% Sodium hydroxide solution followed by ethanol or acidic precipitation affording crude extracts DQW1, DQA1, DQN1 and DQN2 Purification of the crude extracts by dialysis through membrane (MWCO 12 400) resulted to semi-purified extracts DQW1AP, DQA1AP and DQN1AP. Separation by charge and size were done using DEAE-cellulose column and Sephadex G-100 or Sepharose CL-4B. This resulted to water soluble neutral (DQW1AP-1A) and acidic (DQW1AP-2A), ammonium oxalate soluble (DQA1AP-1) and two alkali soluble (DQN1AP-1 and DQN1AP-2) glycan fractions.
Characterization of the hydrolyzed samples using HPAEC-PAD and MALDI TOF MS showed glucan-rich heteroglycans (DQW1AP-1A and DQW1AP-2A) galactan-rich heteroglycan (DQA1AP-1) and glucan and mannan rich heteroglycan, (DQN1AP-1). The hydrolysates had approximately 4-5 degree of polymerization with molecular weights ranging from 689.680 to 851.788 Da. The hydrolysate of fraction DQN1AP-2 is a glucan-rich heteroglycan which also contain N-acetyl glucosamine units. It is composed of polymers with 4-5 hexose units as well as N-acetyl glucosamine containing polymers of 6-8 hexose units with molecular weight range of 689.799 to 851.915 Da and 1055.718 to 1379.584 Da respectively
PhD chemistry graduate seminar series on medicinal chemistry
The Chemistry Department College of Science invited the academic community to the PhD Chemistry Graduate Seminar Series on Medicinal Chemistry on Wednesday, September 22, 2021 1800-1940H
Title Speaker
6:10-6:50 Virtual Screening of Natural Products Library for M2(S31N) Inhibitor
Melody Clerigo (PhD Chemistry Student)
6:50-7:30 Use of peptidomimetics to develop potential SARS-CoV-2 therapeutics
Sarah Diane C. Ong (PhD Chemistry Student
Extraction, isolation and MALDI-QTOF MS/MS analysis of β-D-Glucan from the fruiting bodies of Daedalea quercina
© 2016 Elsevier B.V. We report for the first time the extraction, isolation, and the proposed structure of a polysaccharide from the fruiting bodies of Daedalea quercina. The monosaccharide composition of D. quercina isolate (DQW1Pa1) was mainly glucose as identified using GC–MS. FTIR-ATR spectroscopy and absolute configuration studies showed that this polysaccharide is a β-D-glucan. Its average molecular weight obtained using size exclusion chromatography was 1.6 × 104 Da, consistent with glucans derived from the order Polyporaceae. MALDI-QTOF MS/MS was carried out to identify the linkage and connectivity of the glucose units. Collision Induced Dissociation (CID) of selected parent ions of different oligosaccharide lengths showed the presence of characteristic glycosidic bond cleavages Bn/Cn, the linear backbone by 1-6 linkage, and the cross-ring fragment, 0,3An. Presence of branching unit was identified from high intensity 0,3A4 fragment and verified from diagnostic ion of [D] and [D-H2O] types. To confirm the linkage assignment obtained using MALDI-QTOF MS/MS, DQW1Pa1 was subjected to methylation analysis. Results showed the presence of 1-3, 1-6, 1- and 1-3-6 linked glucose in the order of decreasing abundance, respectively. The repeating unit of isolate DQW1Pa1 was deduced as 1-3 linked linear glucose backbone with branches composed of three 1-3 linked glucose units connected to backbone by 1-6 linkage
Anti-hyperglycemic effects of aqueous Lenzites betulina extracts from the Philippines on the blood glucose levels of the ICR mice (Mus musculus)
Objective: To examine the anti-hyperglycemic effects of aqueous Lenzites betulina (L. betulina) extracts on normoglycemic glucose-loaded mice. Methods: Different doses of aqueous extract from L. betulina were administered to 45 ICR mice (Mus musculus) to determine whether there was an effect of L. betulina extracts on the blood glucose level of the ICR mice. Aqueous extracts of L. betulina were orally gavaged to mice using oral glucose tolerance test. A total of five groups were used to determine the effect of the fungi on blood glucose of the mice. Group A (positive control) was given 16.7 μg/kg glimepiride; Group B (negative control) was given distilled water; Group C (low dosage) was given 200 mg/kg aqueous extract; Group D (mid dosage) was given 400 mg/kg aqueous extract and Group E (high dosage) was given 800 mg/kg aqueous extract. Baseline blood glucose value was firstly acquired before induction of hyperglycemia through d-glucose, after which another check on blood glucose was made after 0.5 h. Immediately, after the acquisition of hyperglycemic blood glucose level, the individual administration of treatments were done. After that, three blood collections were done spanning 3 h with 1 h interval. Results: The low dose (200 mg/kg) and the mid dose (400 mg/kg) of L. betulina extracts were significantly different (P \u3c 0.05) from their respective baseline values throughout the whole experiment with the latter surpassing its baseline value during the 3rd hour. On the other hand, the high dose (800 mg/kg) during the 1st hour after administration was not significantly different (P \u3e 0.05) from its corresponding baseline value, acting faster than the positive control (glimepiride), which only became significantly different (P \u3c 0.05) at the 2nd hour. Conclusions: Aqueous L. betulina extract is able to produce hypoglycemic effects on the mice with all doses, which are able to normalize blood glucose levels at varying times. © 2016 Hainan Medical University