2 research outputs found
Biophysical Measurements of Cells, Microtubules, and DNA with an Atomic Force Microscope
Atomic force microscopes (AFMs) are ubiquitous in research laboratories and
have recently been priced for use in teaching laboratories. Here we review
several AFM platforms (Dimension 3000 by Digital Instruments, EasyScan2 by
Nanosurf, ezAFM by Nanomagnetics, and TKAFM by Thorlabs) and describe various
biophysical experiments that could be done in the teaching laboratory using
these instruments. In particular, we focus on experiments that image biological
materials and quantify biophysical parameters: 1) imaging cells to determine
membrane tension, 2) imaging microtubules to determine their persistence
length, 3) imaging the random walk of DNA molecules to determine their contour
length, and 4) imaging stretched DNA molecules to measure the tensional force.Comment: 29 page preprint, 7 figures, 1 tabl