Retrospective study of 149 cases of salivary gland carcinoma in a Spanish hospital population

The clinical and histological characteristics of salivary gland tumors vary widely, complicating their diagnosis and management, and major differences have been recorded in the distribution of histopathological diagnoses among different countries. This retrospective study reviewed the demographic (age, sex) and clinicopathological (pathology diagnosis and localization) characteristics of cases diagnosed with primary SGC between June 1992 and May 2014 in the Pathology Department of the 12 de Octubre Hospital of Madrid. Diagnoses were recorded according to the 2005 WHO classification. The study included 149 SCG patients, aged between 11 and 94 yrs, with mean age at onset of 55.56 yrs and peak incidence in the eighth decade of life. The male:female ratio was 1.01. The parotid gland was the most frequently involved (75.2%). The most frequent carcinoma was mucoepidermoid carcinoma (24.2%), followed by acinic cell carcinoma (15.4%). The demographic and histopathological characteristics of patients with salivary gland carcinomas in Spain, reported here for the first time, are broadly similar to those found in other countries

Extramedullary plasmacytoma in the maxillofacial region: A review of the literature and case report

Extramedullary Plasmacytoma is an uncommon cell dyscrasia, representing 1% of all head and neck tumours. Characteristically, EMP is a slowly growing neoplasm with pattern of growth that delays its diagnosis. Secondary to this evolution, most patients present symptoms related to the location of the mass.We present a case of EMP in the maxillofacial region in a 59 years old male, who was treated by our department.Treatment approach is generally personalized to each case. These tumours often appear in complicated anatomic locations. EMPs are radiosensitive, and radiotherapy is often used as a first-line treatment to avoid disfiguring results and potentially eliminate the need for further invasive surgical procedures, but surgery combined with Radiotherapy has demonstrated good results in preventing local recurrence

A comparative study of the expression of cyclin D1, COX-2, and KI-67 in odontogenic keratocyst vs. ameloblastoma vs. orthokeratinized odontogenic cyst

Se trata de un RCT (ensayo clínico aleatorizado) sobre tumores odontgénicos mas prevalentes de los maxilares. Los queratoquistes odontogénicos (OKC) y los quistes odontogénicos ortoqueratinizados (OOC) son entidades clinicopatológicas distintas. El OKC parece comportarse de una manera más similar a la de una neoplasia, como el ameloblastoma (AB). El objetivo de este estudio es comparar la influencia de Ki-67, ciclina D1 y COX-2 en el diagnóstico y patogénesis de OKC, OOC y AB. Se realizó un estudio observacional transversal de 41 muestras organizadas en 3 grupos: (1) OKC n=22; (2) AB n=13 y (3) OOC n=6. Los bloques de parafina se seccionaron y se tiñeron con hematoxilina y eosina (H&E). Se realizó un estudio inmunohistoquímico utilizando el kit de detección Bond Polymer Refine Red, Leica, Wetzlar, Alemania, para los siguientes anticuerpos: Ki-67, ciclina D1 y COX-2. La inmunotinción doble ciego se cuantificó subjetivamente. La expresión de Ki-67 fue mayor en el grupo OKC que en el grupo AB (p<0,05). La ciclina D1 mostró una mayor expresión en OKC vs. OOC y OKC vs. AB (p<0.05). Finalmente, la expresión de COX-2 fue mayor en OKC vs AB (p<0.05).La COX-2, el Ki-67 y la ciclina D1 muestran diferencias estadísticamente significativas entre los grupos, lo que sugiere que podrían ser herramientas útiles en el diagnóstico diferencial entre OKC y OOC y un indicador predictivo de su comportamiento biológico. Las mayores expresiones de estos 3 marcadores de OKC vs AB ponen de manifiesto una vez más el comportamiento agresivo de esta lesión quística ahora reconsiderada. Estos marcadores podrían resultar útiles en la elección de un tratamiento quirúrgico más agresivo en los OKC a medida que se presenta su comportamiento.Depto. de Especialidades Clínicas OdontológicasFac. de OdontologíaTRUEpu

Med Oral Patol Oral Cir Bucal-AHEAD OF PRINT -ARTICLE IN PRESS Salivary gland carcinoma in Madrid Retrospective study of 149 cases of salivary gland carcinoma in a Spanish hospital population

Abstract Background: The clinical and histological characteristics of salivary gland tumors vary widely, complicating their diagnosis and management, and major differences have been recorded in the distribution of histopathological diagnoses among different countries. Results: The study included 149 SCG patients, aged between 11 and 94 yrs, with mean age at onset of 55.56 yrs and peak incidence in the eighth decade of life. The male:female ratio was 1.01. The parotid gland was the most frequently involved (75.2%). The most frequent carcinoma was mucoepidermoid carcinoma (24.2%), followed by acinic cell carcinoma (15.4%). Conclusions: The demographic and histopathological characteristics of patients with salivary gland carcinomas in Spain, reported here for the first time, are broadly similar to those found in other countries

Loss of the Mitochondrial Bioenergetic Capacity Underlies the Glucose Avidity of Carcinomas

Article available at http://dx.doi.org/10.1158/0008-5472.CAN-07-1678The down-regulation of the catalytic subunit of the mitochondrial H+-ATP synthase (ß-F1-ATPase) is a hallmark of most human carcinomas. This characteristic of the cancer cell provides a proteomic signature of cellular bioenergetics that can predict the prognosis of colon, lung, and breast cancer patients. Here we show that the in vivo tumor glucose uptake of lung carcinomas, as assessed by positron emission tomography in 110 patients using 2-deoxy-2-[18F]fluoro-D-glucose as probe, inversely correlates with the bioenergetic signature determined by immunohistochemical analysis in tumor surgical specimens. Further, we show that inhibition of the activity of oxidative phosphorylation by incubation of cancer cells with oligomycin triggers a rapid increase in their rates of aerobic glycolysis. Moreover, we show that the cellular expression level of the ß-F1-ATPase protein of mitochondrial oxidative phosphorylation inversely correlates (P < 0.001) with the rates of aerobic glycolysis in cancer cells. The results highlight the relevance of the alteration of the bioenergetic function of mitochondria for glucose capture and consumption by aerobic glycolysis in carcinomasThis work was supported by grants from the Ministerios de Sanidad (PI041255) y Educación y Ciencia (SAF05-4001), Comunidad de Madrid (S-GEN-0269), Indas Biotech S.L., Fundación Investigación Biomédica Hospital Universitario 12 de Octubre and Fundación Mutua Madrileña.Peer reviewe

Oral pulse granuloma and oral pulse granuloma associated with odontogenic keratocyst: Two clinical cases and a review of the literature

En este artículo se presentan 2 casos de granuloma oral pulse (GOP) o granuloma vegetal, uno de ellos asociado a un queratoquiste odontogénico. Esta rara entidad está caracterizada por la presencia de estructuras hialinas en anillo que incluyen vasos, células gigantes, otras células inflamatorias y haces de fibras de colágeno. Sobre su origen todavía se barajan 2 hipótesis: una en la que se sospecha que se producen como reacción a estructuras vegetales como legumbres (de donde toma el nombre de «pulse» o semilla comestible de una leguminosa), y otra en la que se trataría de un cambio degenerativo de las paredes vasculares, resultado de una vasculitis localizada. Debido a la apariencia engañosa del GOP es fácil que a los patólogos les suponga un esfuerzo su diagnóstico diferencial definitivo que mejore la actitud terapeutica. Se describe a continuación un hallazgo inusual de un GOP relacionado con un queratoquiste odontogénico.Depto. de Especialidades Clínicas OdontológicasFac. de OdontologíaTRUEpu

Absence of allelic imbalance involving EMSY, CAPNS, and PAK1 genes in papillary thyroid carcinoma

Papillary thyroid cancer (PTC) accounts for 80% of all thyroid malignancies, and genetic alterations associated to its etiology remain largely unknown. Chromosomal band 11q13 seems to be one of the most frequently amplified regions in human cancer, providing several candidate genes that need detailed characterization. The aim of our study was to investigate the existence of allelic imbalance at EMSY, CAPN5, and PAK1, as candidate genes within 11q13.5-q14 region using a single nucleotide polymorphism-based analysis. We selected a panel of 9 polymorphisms that were analyzed in 41 thyroid carcinoma samples, their contralateral non-pathological tissue and 178 controls from the general population. We did not detect allelic imbalance at these loci in our series. However, we observed a difference in the EMSY-haplotype distribution among PTC patients when compared to controls (odds ratio=2.00; p=0.02). We conclude that 11q13.5-q14 is not imbalanced in PTC, but there is evidence suggesting that EMSY might be of relevance in PTC etiology.Fundación de Investigación Biomédica Mutua Madrileña AutomovilistaMinisterio de Ciencia y TecnologíaDepto. de CirugíaFac. de MedicinaTRUEpu

Biochemical basis of a novel T-lymphocyte receptor immunodeficiency by immunohistochemistry: A possible CD3γ abnormality

Necropsic lymphoid tissues obtained from an infant with a novel type of immunodeficiency consisting of a peripheral blood T lymphocyte antigen receptor (TCR) surface expression defect, were analyzed by immunohistochemistry for the expression of various TCR-associated epitopes. The work was aimed to characterize the biochemical basis of this kind of disorder and confirm the defect in different lymphoid tissues. Within an assessed lymphoid depletion, the patient's tissues showed a normal expression of several TCR epitopes (those associated to CD3 epsilon, CD3 delta and the clonotypic -Ti- alpha and beta chains). In contrast, the expression of the epitopes recognized by the monoclonals OKT3, WT31, and BMA031 was severely diminished. Our results therefore support that CD3 epsilon, CD3 delta, Ti alpha and Ti beta are probably not involved in this type of immunodeficiency, and strongly suggest that CD3 gamma (forming part of the epitope recognized by OKT3) may rather be the affected chain giving rise to the defective surface T cell phenotype; however, alternative interpretations are not ruled out. The disrupted TCR thus formed, containing Ti alpha beta heterodimers and CD3 epsilon and CD3 delta subunits, but lacking normal CD3 gamma, would in this scheme lack the conformational framework determinants recognized by WT31 and BMA031.Depto. de Inmunología, Oftalmología y ORLFac. de MedicinaTRUEpu