Surface acoustic wave-driven pumpless flow for sperm rheotaxis analysis

Sperm rheotaxis, the phenomenon where sperm cells swim against the direction of fluid flow, is one of the major guiding mechanisms for long-distance sperm migration within the female reproductive tract. However, current approaches to study this pose challenges in dealing with rare samples by continuously introducing extra buffer. Here, we developed a device utilising acoustic streaming, the steady flow driven by an acoustic perturbation, to drive a tuneable, well-regulated continuous flow with velocities ranging from 40 μm s−1 to 128 μm s−1 (corresponding to maximum shear rates of 5.6 s−1 to 24.1 s−1) in channels of interest - a range suitable for probing sperm rheotaxis behaviour. Using this device, we studied sperm rheotaxis in microchannels of distinct geometries representing the geometrical characteristics of the inner-surfaces of fallopian tubes, identified sperm dynamics with the presence of flow in channels of various widths. We found a 28% higher lateral head displacement (ALH) in sufficiently motile rheotactic sperm in a 50 μm channel in the presence of acoustically-generated flow as well as a change in migration direction and a 52% increase in curvilinear velocity (VCL) of sufficiently motile sperm in a 225 μm channel by increasing the average flow velocity from 40 μm s−1 to 130 μm s−1. These results provided insights for understanding sperm navigation strategy in the female reproductive tract, where rheotactic sperm swim near the boundaries to overcome the flow in the female reproductive tract and reach the fertilization site. This surface acoustic wave device presents a simple, pumpless alternative for studying microswimmers within in vitro models, enabling the discovery of new insights into microswimmers' migration strategies, while potentially offering opportunities for rheotaxis-based sperm selection and other flow-essential applications.</p

Trapping and patterning of large particles and cells in a 1D ultrasonic standing wave

Acoustic forces can be used to pattern single particles; here, the required relationship between wavelength and particle radius is explored.</p

Acoustic tweezing of particles using decaying opposing travelling surface acoustic waves (DOTSAW)

Surface acoustic waves offer a versatile and biocompatible method of manipulating the location of suspended particles or cells within microfluidic systems.</p

On-demand sample injection: combining acoustic actuation with a tear-drop shaped nozzle to generate droplets with precise spatial and temporal control

An on-demand droplet injection method for controlled delivery of nanolitre-volume liquid samples to scientific instruments for subsequent analysis is presented.</p

The role of channel height and actuation method on particle manipulation in surface acoustic wave (SAW)-driven microfluidic devices

AbstractSurface acoustic wave (SAW) micromanipulation offers modularity, easy integration into microfluidic devices and a high degree of flexibility. A major challenge for acoustic manipulation, however, is the existence of a lower limit on the minimum particle size that can be manipulated. As particle size reduces, the drag force resulting from acoustic streaming dominates over acoustic radiation forces; reducing this threshold is key to manipulating smaller specimens. To address this, we investigate a novel excitation configuration based on diffractive-acoustic SAW (DASAW) actuation and demonstrate a reduction in the critical minimum particle size which can be manipulated. DASAW exploits the inherent diffractive effects arising from a limited transducer area in a microchannel, requiring only a travelling SAW (TSAW) to generate time-averaged pressure gradients. We show that these acoustic fields focus particles at the channel walls, and further compare this excitation mode with more typical standing SAW (SSAW) actuation. Compared to SSAW, DASAW reduces acoustic streaming effects whilst generating a comparable pressure field. The result of these factors is a critical particle size with DASAW (1 \upmu μ m) that is significantly smaller than that for SSAW actuation (1.85 \upmu μ m), for polystyrene particles and a given $$\lambda _{\text {SAW}}$$ λ SAW = 200 \upmu μ m. We further find that streaming magnitude can be tuned in a DASAW system by changing the channel height, noting optimum channel heights for particle collection as a function of the fluid wavelength at which streaming velocities are minimised in both DASAW and SSAW devices.</jats:p

Batch process particle separation using surface acoustic waves (SAW):Integration of travelling and standing SAW

Acoustic fields are described incorporating travelling and standing wave components to perform size-deterministic particle sorting. This is achieved without the need for fluid flow allowing application to very small volumes in a batch-wise system.</p