Estimation of body density in adolescent athletes

National samples of 141 male and 133 female highly-trained adolescent athletes were studied to derive anthropometric-based equations predicting body density. Anthropometric measures included skinfold thicknesses at seven sites, circum- ferences at 14 sites, and diameters at nine sites. Criterion measures of body density were determined by underwater weighing with corrections for residual lung vol- ume based on the oxygen dilution method. Variable selection procedures included factor analysis followed by forward-stepping regression and polynomial analysis. For both the male and female samples, two quadratic equations utilizing either the sum of three or seven skinfold measures were derived. Within the male sample, high validity coefficients (R = 0.81 - 0.82) and low standard errors (SEE = 0.0055 - 0.0056 g-ml !) were shown with these equations. Similar results were demon- strated with the equations for females (R = 0.82 and SEE = 0.0060 g-ml~ l). Cross- validation on independent samples of male (n = 66) and female (n = 46) adolescent athletes further confirmed these findings. In the cross-validation sample of males, predicted scores were highly correlated with actual body density (r = 0.86 - 0.87) and the total error of prediction ranged from 0.0057 to 0.0061 g-ml~ l. Among the females, these values were r = 0.82 - 0.83 and total error = 0.0058 to 0.0063 g*ml-1. These results indicate that within reasonable limits of error, the sum of three or seven skinfolds may be used to make estimates of the body density of adolescent male or female athlete

Streptococcal Receptor Polysaccharides: Recognition Molecules for Oral Biofilm Formation

<p>Abstract</p> <p>Background</p> <p>Strains of viridans group <it>streptococci </it>that initiate colonization of the human tooth surface typically coaggregate with each other and with <it>Actinomyces naeslundii</it>, another member of the developing biofilm community. These interactions generally involve adhesin-mediated recognition of streptococcal receptor polysaccharides (RPS). The objective of our studies is to understand the role of these polysaccharides in oral biofilm development.</p> <p>Methods</p> <p>Different structural types of RPS have been characterized by their reactions with specific antibodies and lectin-like adhesins. Streptococcal gene clusters for RPS biosynthesis were identified, sequenced, characterized and compared. RPS-producing bacteria were detected in biofilm samples using specific antibodies and gene probes.</p> <p>Results</p> <p>Six different types of RPS have been identified from representative viridans group <it>streptococci </it>that coaggregate with <it>A. naeslundii</it>. Each type is composed of a different hexa- or heptasaccharide repeating unit, the structures of which contain host-like motifs, either GalNAcβ1-3Gal or Galβ1-3GalNAc. These motifs account for RPS-mediated recognition, whereas other features of these polysaccharides are more closely associated with RPS antigenicity. The RPS-dependent interaction of <it>S. oralis </it>with <it>A. naeslundii </it>promotes growth of these bacteria and biofilm formation in flowing saliva. Type specific differences in RPS production have been noted among the resident streptococcal floras of different individuals, raising the possibility of RPS-based differences in the composition of oral biofilm communities.</p> <p>Conclusion</p> <p>The structural, functional and molecular properties of streptococcal RPS support a recognition role of these cell surface molecules in oral biofilm formation.</p

Environmental movements in space-time: the Czech and Slovak republics from Stalinism to post-socialism

To demonstrate the role of space and time in social movements, the paper analyses the evolution and context of the environmental movement in the Czech and Slovak republics from 1948 to 1998. It shows that the movement's identity was formed under socialism and that political opportunity and resource availability changed markedly over time, as did its organisational and spatial structure. The movement played a significant part in the collapse of the socialist regime, but in the 1990s was marginalised in the interests of building a market economy and an independent Slovakia. Nevertheless a diverse and flexible range of groups existed by the late 1990s. The successive space-times allow analysis of the multiple and changing variables that influence the geography of social movements

Lack of the Delta Subunit of RNA Polymerase Increases Virulence Related Traits of Streptococcus mutans

The delta subunit of the RNA polymerase, RpoE, maintains the transcriptional specificity in Gram-positive bacteria. Lack of RpoE results in massive changes in the transcriptome of the human dental caries pathogen Streptococcus mutans. In this study, we analyzed traits of the ΔrpoE mutant which are important for biofilm formation and interaction with oral microorganisms and human cells and performed a global phenotypic analysis of its physiological functions. The ΔrpoE mutant showed higher self-aggregation compared to the wild type and coaggregated with other oral bacteria and Candida albicans. It formed a biofilm with a different matrix structure and an altered surface attachment. The amount of the cell surface antigens I/II SpaP and the glucosyltransferase GtfB was reduced. The ΔrpoE mutant displayed significantly stronger adhesion to human extracellular matrix components, especially to fibronectin, than the wild type. Its adhesion to human epithelial cells HEp-2 was reduced, probably due to the highly aggregated cell mass. The analysis of 1248 physiological traits using phenotype microarrays showed that the ΔrpoE mutant metabolized a wider spectrum of carbon sources than the wild type and had acquired resistance to antibiotics and inhibitory compounds with various modes of action. The reduced antigenicity, increased aggregation, adherence to fibronection, broader substrate spectrum and increased resistance to antibiotics of the ΔrpoE mutant reveal the physiological potential of S. mutans and show that some of its virulence related traits are increased

Cloning and nucleotide sequence of a gene for Actinomyces naeslundii WVU45 type 2 fimbriae.

A genomic library of Actinomyces naeslundii WVU45 DNA in Escherichia coli was screened for antigen expression with rabbit antibody against A. naeslundii fimbriae. Western blotting (immunoblotting) of one recombinant clone carrying a 13.8-kilobase-pair insert revealed a 59-kilodalton (kDa) immunoreactive protein. A protein of similar electrophoretic mobility was detected from the isolated fimbrial antigen. Expression of the 59-kDa cloned protein in E. coli was directed by a promoter from the insert. The DNA sequence of the subunit gene was determined, and an open reading frame of 1,605 nucleotides was identified which was preceded by a putative ribosome-binding site and followed by two inverted repeats of 14 and 17 nucleotides, respectively. The reading frame encoded a protein of 534 amino acids (calculated molecular weight, 57,074), and the N-terminal sequence resembled that of a signal peptide. The presence of a 32-amino-acid signal peptide was indicated by amino-terminal sequencing of the fimbriae from A. naeslundii. The sequence, as determined by Edman degradation, was identical to that deduced from the DNA sequence beginning at predicted residue 33 of the latter sequence. Moreover, the amino acid composition of the predicted mature protein was similar to that of the isolated fimbriae from A. naeslundii. Thus, the cloned gene encodes a subunit of A. naeslundii fimbriae