546 research outputs found
DENDRITIC CELL DIFFERENTIATION BLOCKED BY PRIMARY EFFUSION LYMPHOMA-RELEASED FACTORS IS PARTIALLY RESTORED BY INHIBITION OF P38 MAPK
To better understand the molecular mechanisms underlying the dendritic cell (DC) defects in cancer, we analyzed which signaling pathway is implicated in the abnormal monocyte differentiation into DC determined by the presence of Primary effusion lymphoma (PEL) released factors. Our results indicate that the DC, obtained in this condition, together with phenotypic abnormalities and reduced allostimulatory function, showed hyperphosphorylation of signal transducer and activator of transcription 3 (STAT3) and p38 mitogen-activated protein kinase (MAPK) molecules, in comparison to the DC differentiated in the absence of PEL-released factors. The inhibition of p38 MAPK but not of STAT3 phosphorylation, with specific inhibitors, was able to revert the effect of the PEL-released factors on the DC phenotype. This study suggests that p38 MAPK signaling pathway is an important contributor to the abnormal differentiation of DC in PEL
Collective decoherence of cold atoms coupled to a Bose-Einstein condensate
We examine the time evolution of cold atoms (impurities) interacting with an
environment consisting of a degenerate bosonic quantum gas. The impurity atoms
differ from the environment atoms, being of a different species. This allows
one to superimpose two independent trapping potentials, each being effective
only on one atomic kind, while transparent to the other. When the environment
is homogeneous and the impurities are confined in a potential consisting of a
set of double wells, the system can be described in terms of an effective
spin-boson model, where the occupation of the left or right well of each site
represents the two (pseudo)-spin states. The irreversible dynamics of such
system is here studied exactly, i.e., not in terms of a Markovian master
equation. The dynamics of one and two impurities is remarkably different in
respect of the standard decoherence of the spin - boson system. In particular
we show: i) the appearance of coherence oscillations, i) the presence of super
and sub decoherent states which differ from the standard ones of the spin boson
model, and iii) the persistence of coherence in the system at long times. We
show that this behaviour is due to the fact that the pseudospins have an
internal spatial structure. We argue that collective decoherence also prompts
information about the correlation length of the environment. In a one
dimensional configuration one can change even stronger the qualitative
behaviour of the dephasing just by tuning the interaction of the bath.Comment: 18 pages, 6 figures, two references adde
Casimir-Polder interatomic potential between two atoms at finite temperature and in the presence of boundary conditions
We evaluate the Casimir-Polder potential between two atoms in the presence of
an infinite perfectly conducting plate and at nonzero temperature. In order to
calculate the potential, we use a method based on equal-time spatial
correlations of the electric field, already used to evaluate the effect of
boundary conditions on interatomic potentials. This method gives also a
transparent physical picture of the role of a finite temperature and boundary
conditions on the Casimir-Polder potential. We obtain an analytical expression
of the potential both in the near and far zones, and consider several limiting
cases of interest, according to the values of the parameters involved, such as
atom-atom distance, atoms-wall distance and temperature.Comment: 11 page
Chloroquine supplementation increases the cytotoxic effect of curcumin against Her2/neu overexpressing breast cancer cells in vitro and in vivo in nude mice while counteracts it in immune competent mice
Autophagy is usually a pro-survival mechanism in cancer cells, especially in the course of chemotherapy, thus autophagy inhibition may enhance the chemotherapy-mediated anti-cancer effect. However, since autophagy is strongly involved in the immunogenicity of cell death by promoting ATP release, its inhibition may reduce the immune response against tumors, negatively influencing the overall outcome of chemotherapy. In this study, we evaluated the in vitro and in vivo anti-cancer effect of curcumin (CUR) against Her2/neu overexpressing breast cancer cells (TUBO) in the presence or in the absence of the autophagy inhibitor chloroquine (CQ). We found that TUBO cell death induced by CUR was increased in vitro by CQ and slightly in vivo in nude mice. Conversely, CQ counteracted the Cur cytotoxic effect in immune competent mice, as demonstrated by the lack of in vivo tumor regression and the reduction of overall mice survival as compared with CUR-treated mice. Immunohistochemistry analysis revealed the presence of a remarkable FoxP3 T cell infiltrate within the tumors in CUR/CQ treated mice and a reduction of T cytotoxic cells, as compared with single CUR treatment. These findings suggest that autophagy is important to elicit anti-tumor immune response and that autophagy inhibition by CQ reduces such response also by recruiting T regulatory (Treg) cells in the tumor microenvironment that may be pro-tumorigenic and might counteract CUR-mediated anti-cancer effects
Glucose restriction induces cell death in parental but not in homeodomain-interacting protein kinase 2-depleted RKO colon cancer cells: molecular mechanisms and implications for tumor therapy.
Tumor cell tolerance to nutrient deprivation can be an important factor for tumor progression, and may depend on deregulation of both oncogenes and oncosuppressor proteins. Homeodomain-interacting protein kinase 2 (HIPK2) is an oncosuppressor that, following its activation by several cellular stress, induces cancer cell death via p53-dependent or -independent pathways. Here, we used genetically matched human RKO colon cancer cells harboring wt-HIPK2 (HIPK2(+/+)) or stable HIPK2 siRNA interference (siHIPK2) to investigate in vitro whether HIPK2 influenced cell death in glucose restriction. We found that glucose starvation induced cell death, mainly due to c-Jun NH2-terminal kinase activation, in HIPK2(+/+)cells compared with siHIPK2 cells that did not die. (1)H-nuclear magnetic resonance quantitative metabolic analyses showed a marked glycolytic activation in siHIPK2 cells. However, treatment with glycolysis inhibitor 2-deoxy-D-glucose induced cell death only in HIPK2(+/+) cells but not in siHIPK2 cells. Similarly, siGlut-1 interference did not re-establish siHIPK2 cell death under glucose restriction, whereas marked cell death was reached only after zinc supplementation, a condition known to reactivate misfolded p53 and inhibit the pseudohypoxic phenotype in this setting. Further siHIPK2 cell death was reached with zinc in combination with autophagy inhibitor. We propose that the metabolic changes acquired by cells after HIPK2 silencing may contribute to induce resistance to cell death in glucose restriction condition, and therefore be directly relevant for tumor progression. Moreover, elimination of such a tolerance might serve as a new strategy for cancer therapy
Casimir-Polder potentials as entanglement probe
We have considered the interaction of a pair of spatially separated two-level
atoms with the electromagnetic field in its vacuum state and we have analyzed
the amount of entanglement induced between the two atoms by the non local field
fluctuations. This has allowed us to characterize the quantum nature of the non
local correlations of the electromagnetic field vacuum state as well as to link
the induced quantum entanglement with Casimir-Polder potentials.Comment: Published on Europhysics Letters 78 (2007) 3000
Excitations of attractive 1-D bosons: Binding vs. fermionization
The stationary states of few bosons in a one-dimensional harmonic trap are
investigated throughout the crossover from weak to strongly attractive
interactions. For sufficient attraction, three different classes of states
emerge: (i) N-body bound states, (ii) bound states of smaller fragments, and
(iii) gas-like states that fermionize, that is, map to ideal fermions in the
limit of infinite attraction. The two-body correlations and momentum spectra
characteristic of the three classes are discussed, and the results are
illustrated using the soluble two-particle model.Comment: 7 pages, 5 figure
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