Xylanase - the enzyme which makes the difference in nutrition

Nutrition plays a critical role in health status both for humans and animals, comprising very complex processes starting with the ingestion of food or feed and ending with the excretion. But in this whole chain of processes, one of the main elements is the enzyme system without which digestion could not be possible. Xylanase is one of the enzymes that can make the difference in the digestion and absorption of some carbohydrates, like the digestion and absorption of fibers, used in various fields - food processing, feed additives, biofuel production, paper and pulp industry, textile industry, as well as waste management. Biochemically, xylanase is an enzyme that breaks down xylan, a complex polysaccharide found in plant cell walls. This paper presents some characteristics of xylanase, the important role in biotechnology, and also some applications of this enzyme

Therapeutic properties and phenantrens compounds of Tamus communis

From ancient in folk medicine the plants were used with lots of trust and efficiency. In time, people learn how to cultivate, to sample, and preserve different anatomical parts of the plant for medical purposes. Thus, also Tamus communis became an interesting plant, being used in different pharmaceutical formulations to treat different medical conditions. The extracts of root of T. communis is just one phyto-pharmaceutical form that demonstrated to have antiinflammatory and antioxidant effect. Different anatomical parts of T. communis, various extraction methods using various solvents, were used in experimental researches to test the efficiency as cytotoxic and antiviral activities, antioxidant activity, anti-inflammatory and analgesic activity. Extracts of T. communis are used for preparation of poultice, ointments, and also for infusions, tincture preparation with very good pharmacologic activity. This paper only made a very succinct presentation of the possibilities to used Tamus communis as a main ingredient of different natural pharmacological preparation with applicability in human and veterinary medicine

Histomorphometry of Deldoid Muscle (Musculus Deltoideus) at the Hybrids of Mouflon (Ovis Ammon Musimon Pal.) and Domestic Sheep

The aim of this study was to establish production performances upon slaughter in the hybrids obtained by crossbreeding of the mouflon (Ovis ammon musimon Pal.) and domestic sheep. The morphological and histomorphometric study was carried out on fragments deltoid muscles (Musculus deltoideus) sampled from seven MM x FT hybrids and seven MT x FM hybrids, respectively. To do so, muscle fragments were fixed in a 10% formalin solution and histological preparations were stained through the Mallory trichromic method. Histomorphometric analysis reveals that the MM x FT hybrids have thicker muscular fibbers and smaller amounts of inter-fibrillar connective tissue than the MT x FM hybrids (p0.05). Results of our study show that using mouflon (Ovis ammon musimon Pal.) and local Tigaie sheep in the development of new genotypes could be a good solution for both the conservation of these threatened species and production of high quality mutton

Antiproliferative Effects of Tetrabuthylammonium Chloride Ionic Liquid on HCT 8 Human Colon Carcinoma Cells

The ionic liquids have attracted a great of attention in the scientific community due to their potential pharmaceutical such as antimicrobial. In this paper, the main objective was the assessment of the cytotoxic effect of tetrabutylammonium chloride against HCT 8 human colon carcinoma cell line. The cells were cultured in 75 cm2 culture flasks  using RPMI medium supplemented with 10% inactivated fetal bovine serum (FBS), penicillin (100 IU/mL) and streptomycin (100 μg/mL) and maintained at 37 °C and 5% CO2. Before achieving viability test, the cells were harvested using trypsin solution (0.25%). Then, the cells were seeded in 24 – well plates at a density of 5 x 105 cells/mL in 100 µL medium/well in order to reach confluence. After 24 h, the medium was replaced with fresh medium containing different concentrations of ionic liquid, respectively, 0.085, 0.17, 0.34, 0.68 and 1.36 mg /mL. Control group contained cells without treatment. Cell proliferation kinetics have been studied at 24 and 48 h after IL treatment, following trypsinization and counting total cells per plate by using a Trypan blue dye and a hemocytometer. Data obtained from the growth kinetics assay shows that the tetrabutylammonium chloride (TBAC) had an inhibitory effect on the growth of cells in a concentration dependent manner. The maximum inhibitory effect on HCT 8 cells it was obtained at 1.36 mg TBAC/mL

Comparative Studies on Slaughter Performances of the European Mouflon (Ovis orientalis musimon Lin.) and the Hybrids Obtained by Crossbreeding between Mouflon and Sheep Breed Tigaie

The aim of this work was to determine slaughter performances and carcass characteristics at European Mouflon (Ovis orientalis musimon Lin.). Also, for studied characteristics were determined the differences between European Mouflon (Ovis orientalis musimon Lin.) and hybrids (F1) obtained by crossbreeding between European Mouflon (Ovis ammon musimon Pal.) and the Romanian sheep breed Tigaie, raised under identical conditions. First generation hybrids (F1) were obtained by crossbreeding between females of sheep breed Tigaie and males of mouflon (FT x MM). The studied characteristics were slaughter yield on hot and chilled carcass, the participation percentage of the cut parts from live weight and from the whole carcass structure. At the age of 90 days 10 lambs were sacrificed (5 mouflons and 5 hybrids FTxMM). For slaughter performances, superior values were obtained from the hybrid lambs. Thus, slaughter yield calculated had higher at FTxMM (P<0.05) as compared with values obtained at mouflon. The participation percentage of the cut parts from the live weight and from the whole carcass structure had the lower values at mouflon lambs compared to FTxMM hybrids. Results from this study showed that hybrid genotype significantly influenced the studied characteristics and have revealed the superiority of the hybrids lambs against mouflon lambs