Zanthoxylum bungeanum seed oil inhibits tumorigenesis of human melanoma A375 by regulating CDC25A/CyclinB1/CDK1 signaling pathways in vitro and in vivo

Background:Zanthoxylum bungeanum seed oil (ZBSO) is extracted from the seeds of the traditional Chinese medicine Z. bungeanum Maxim, which has been shown to have anti-melanoma effects. However, the specific mechanisms are not illustrated adequately.Aims: To further investigate the mechanism by which ZBSO inhibits melanoma and to provide scientific evidence to support ZBSO as a potential melanoma therapeutic candidate.Methods: CCK-8 assays were used to detect the function of ZBSO on A375 cells. Based on transcriptomics analyses, Western blot analysis was applied to determine whether an association existed in ZBSO with the CDC25A/CyclinB1/CDK1 signaling pathway. In addition, RT-qPCR and immunohistochemistry analysis validated that ZBSO has the anti-melanoma effect in a nude mouse xenograft model of human melanoma. Then, 16S rRNA sequencing was used to detect the regulation of gut microbes.Results: Cellular assays revealed that ZBSO could inhibit A375 cell viability by regulating the cell cycle pathway. Further studies presented that ZBSO could constrain CDC25A/CyclinB1/CDK1 signaling pathway in vitro and in vivo models of melanoma. ZBSO did not produce toxicity in mice, and significantly reduced tumor volume in xenotransplants of A375 cells. Genome analysis indicated that ZBSO successfully altered specific gut microbes.Conclusion: ZBSO inhibited the growth of A375 cells by regulating CDC25A/cyclinB1/CDK1 signaling pathway both in vitro and in vivo, suggesting that ZBSO may be a novel potential therapeutic agent

Marsdenia tenacissima enhances immune response of tumor infiltrating T lymphocytes to colorectal cancer

IntroductionTumor-infiltrating T lymphocytes in the tumor microenvironment are critical factors influencing the prognosis and chemotherapy outcomes. As a Chinese herbal medicine, Marsdenia tenacissima extract (MTE) has been widely used to treat cancer in China. Its immunoregulatory effects on tumor-associated macrophages is well known, but whether it regulates tumor-infiltrating T-cell functions remains unclear.MethodWe collected 17 tumor samples from MTE-administered colorectal cancer patients, 13 of which showed upregulation of CD3+/CD8+ tumor-infiltrating T cells. Further in vitro and in vivo experiments were performed to investigate the regulatory effects of MTE on tumor-infiltrating T cells and immune escape of tumors.ResultsUnder single and co-culture conditions, MTE inhibited TGF-β1 and PD-L1 expression in the colorectal cancer (CRC) cell lines HCT116 and LoVo. In Jurkat cells, MTE inhibited FOXP3 and IL-10 expression, increased IL-2 expression, but had no effect on PD-1 expression. These findings were confirmed in vitro using subcutaneous and colitis-associated CRC mouse models. MTE also increased the density of CD3+/CD8+ tumor-infiltrating T cells and exhibited considerable tumor-suppressive effects in these two tumor mouse models.ConclusionsOur findings suggested that MTE inhibits the immune escape of cancer cells, a precipitating factor increasing the immune response of T lymphocytes

Experimental study of the application of micro-PIV on the flow characteristics detection of micro-gap rotational flow field

For a micro-gap rotational flow field with a large horizontal extent, tiny gap and fast flow velocity, the two-dimensional images shot by the micro-scale Particle ImageVelocimetry(Micro-PIV) technique are not sufficient for the study of local or whole flow characteristics. In this paper, by establishing a test bench of a rotational flow field with the functions of driving, positioning, adjustment and sensing, all the local states of the micro-gap rotational flow field can be obtained by horizontally moving the rotating axis to observe point by point. While measuring some local flow fields, two-dimensional pictures are taken by adjusting the focusing height of the objective lens, and then superposed and interpolated according to their shooting order to obtain a quasi-three-dimensional distribution image of the local flow fields, thus obtaining the flow condition of the vertical section of the flow field. The position of the focusing plane and mutual distance are adjusted to realize the measurement of wall shear force in the flow field, providing a feasible reference method for detecting the rheological property of the gap flow field and the effect of surface drag reduction

Impact of Laser Intensity Noise on Dual-Comb Absolute Ranging Precision

Noise in mode-locked lasers has been a central issue for dual-comb metrological applications. In this work, we investigate the laser intensity noise on dual-comb absolute ranging precision. Two different dual-comb schemes based on linear optical sampling (LOS) and nonlinear asynchronous optical sampling (ASOPS) have been constructed. In the LOS scheme, the ranging precision deteriorates with the increase in laser relative intensity noise (RIN). This effect can be corrected by implementing a balanced photo-detection (BPD). In the ASOPS scheme, the experiment shows that the conversion from laser RIN to dual-comb ranging precision is negligible, making a balanced detection unnecessary for ranging precision improvement. The different manners of RIN’s impact on absolute ranging precision are attributed to the distinct cross-correlation signal patterns and the underlying time-of-flight (TOF) extraction algorithms

Simulation study on clogging of suspended particles in in-situ leaching of uranium at different concentrations and flow velocity

Clogging problem has become one of the key problems restricting the mining efficiency of in-situ leaching of uranium, and the related research on the law and mechanism of physical clogging has not been reported. In order to identify and understand the complicated law and mechanism, experimental device is established to simulate the physical clogging caused by suspended particles in the uranium process, the physical clogging law and mechanism under different concentrations and velocity of flow are studied. The experimental results show that with the concentration of suspended particles increasing from 100, 200, 300 to 400 mg/L, the permeability of porous media gradually decreases, and the clogging phenomenon becomes more and more obvious. When the size of suspended particles is small and the velocity is 15 mL/min, the porous medium will not appear clogging, while the velocity is 25mL/min, the whole porous medium will slowly appear internal deposition clogging. When the size of suspended solids is larger and the flow rate is 9, 12, 15mL/min, the higher the velocity, the faster the clogging will be, and backwash can alleviate the surface clogging but cannot change the final clogging result. According to the experiment and actual situation, the physical clogging in in-situ leaching of uranium is mainly surface clogging and filter clogging

AgCu/SiC-powder: A highly stable and active catalyst for gas-phase selective oxidation of alcohols

Highly active and selective bimetallic AgCu/SiC-powder catalysts have been prepared by a facile method for the gas-phase selective oxidation of alcohols (such as 99.3% benzyl alcohol conversion and 99.6% benzyl aldehyde selectivity). The catalytic activity can maintain at least 200 h compared with Cu/SiC (only within 10 h). The formation of Ag-Cu2O ensembles brings about enhanced improvement both in conversion and stability. The XPS results reveal that large amounts of Ag0-Cu2O active sites formed after pre-activation process and Cu2O sites play a dominant role in benzyl alcohol oxidation reaction. Dehydrogenation reaction process has also been confirmed by O2-TPD

Catalytic ethanolysis and gasification of kraft lignin into aromatic alcohols and H2-rich gas over Rh supported on La2O3/CeO2?ZrO2

Efficient catalytic ethanolysis and gasification of kraft lignin were conducted over a versatile supported catalyst Rh/La2O3/CeO2–ZrO2 to give high-value aromatic alcohols and H2-rich gas. The removal of phenolic hydroxyl group was the most prevalent reaction, and importantly, almost no phenols, undesired char and saturating the aromatic ring were detected. Meanwhile, the feedstock and solvent both played key roles in H2 generation that contributed to the hydrodeoxygenation of liquid components and made the whole catalytic process out of H2 supply. Reusability tests of catalyst indicated that the crystalline phase transition and agglomeration of support, the loss of noble metal Rh and carbon deposition were the possible reasons for its deactivation in supercritical ethanol. Comparing with water, methanol and isopropanol system, ethanol was the only effective solvent for the depolymerization process

Fungus–Fungus Association of <i>Boletus griseus</i> and <i>Hypomyces chrysospermus</i> and Cadmium Resistance Characteristics of Symbiotic Fungus <i>Hypomyces chrysospermus</i>

Fungi bioaccumulation of heavy metals is a promising approach to remediate polluted soil and water. Boletus griseus could accumulate high amounts of Cd, even in a natural habitat with low Cd contents. This study found a symbiotic association of B. griseus with a fungus. The symbiotic fungus was isolated and identified as Hypomyces chrysospermus. The isolated strain had a strong ability to tolerate Cd. The minimum inhibitory concentration of Cd of fungal growth was 200 mg·L−1. The Cd bioaccumulation capacity of the fungus reached 10.03 mg·g−1. The biomass production of the fungus was promoted by 20 mg·L−1 Cd. However, high concentrations of Cd suppressed fungal growth and significantly altered the morphology and fine texture of fungal hyphae and chlamydospores. The immobilization effects of the cell wall and acid compounds and antioxidant enzymes were employed by the fungus to alleviate the toxic effects of Cd. The results not only demonstrate a new insight into the Cd bioconcentration mechanisms of B. griseus but also provide a potential bioremediation fungus for Cd contamination

Fixed-Bed and Mobile-Bed Resistance of Channels with Steep Gradients in Mountainous Areas

Flood discharge and sediment transport are closely linked to channel resistance in steep mountain streams. Previous research has mainly focused on the resistance of fixed-bed channels with steep gradients and mobile-bed channels in alluvial rivers. The present study performs an experiment and establishes a calculation method for the fixed-bed resistance of mountain channels. The basic expression of the mobile-bed resistance of steep mountain channels is derived by determining the controlling factors of the bed load movement on the riverbed resistance. The proposed formula can accurately predict the variation of the bed load resistance. The results of the present research improve the understanding of fluid dynamics and sediment transport in steep mountain channels