Contrast‐Aided Diagnostic Ultrasound Does Not Enhance Lung Metastasis in a Mouse Melanoma Tumor Model

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/135357/1/jum2005243349.pd

Lithotripter Shockwave-Induced Enhancement of Mouse Melanoma Lung Metastasis: Dependence on Cavitation Nucleation

Purpose: To confirm a previous report of metastasis enhancement by lithotripter shockwaves (LSW) and to test the hypothesis that this effect is attributable to cavitation. Materials and Methods: The metastatic B16-D5 melanoma cell line was implanted on the hind legs of female C57/b16 mice 12 days before tumor treatment. The tumors were treated with 500 LSW in a waterbath arrangement. The effect of augmented cavitation nucleation was tested by intratumor injection of air bubbles or ultrasound contrast agent gas bodies (UCAGB). The primary tumor was surgically removed on day 1 after treatment. The six groups of mice were sham, LSW, sham + air bubbles, LSW + air bubbles, sham + UCAGB, and LSW + UCAGB. Data were collected for the 113 mice that survived at least 25 days. Lung evaluations were performed blind after 2 weeks of bleaching in Fekete's solution. Results: The outcomes of the three sham groups were very similar and indicated that the simple injection of material into the tumor did not increase metastasis. In comparison with the pooled shams, both the LSW + air bubbles and LSW + UCAGB groups had statistically significant increases in metastasis counts. Only the LSW + UCAGB group had a significant increase in incidence of metastasis relative to the pooled shams. The LSW + UCAGB also had significantly reduced survival. Conclusion: Shockwaves can enhance metastasis from tumors, and this effect is attributable to cavitation.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/63207/1/end.2004.18.925.pd

Anesthetic Techniques Influence the Induction of Pulmonary Capillary Hemorrhage During Diagnostic Ultrasound Scanning in Rats

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/135630/1/jum2015342289.pd

Cardiac Arrhythmia and Injury Induced in Rats by Burst and Pulsed Mode Ultrasound With a Gas Body Contrast Agent

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/135652/1/jum200928111519.pd

Contrast‐Enhanced Diagnostic Ultrasound Causes Renal Tissue Damage in a Porcine Model

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/135402/1/jum201029101391.pd

Hepatocyte Injury Induced by Contrast‐Enhanced Diagnostic Ultrasound

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/149493/1/jum14883_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/149493/2/jum14883.pd

Do Anesthetic Techniques Influence the Threshold for Glomerular Capillary Hemorrhage Induced in Rats by Contrast‐Enhanced Diagnostic Ultrasound?

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/135638/1/jum2016352373.pd

Pulmonary Capillary Hemorrhage Induced by Acoustic Radiation Force Impulse Shear Wave Elastography in Ventilated Rats

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/151830/1/jum14950.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/151830/2/jum14950_am.pd

Quantitative assessment of damage during MCET: a parametric study in a rodent model

Abstract Background Myocardial cavitation-enabled therapy (MCET) has been proposed as a means to achieve minimally invasive myocardial reduction using ultrasound to produce scattered microlesions by cavitating contrast agent microbubbles. Methods Rats were treated using burst mode focused ultrasound at 1.5 MHz center frequency and varying envelope and pressure amplitudes. Evans blue staining indicated lethal cardiomyocytic injury. A previously developed quantitative scheme, evaluating the histologic treatment results, provides an insightful analysis for MCET treatment parameters. Such include ultrasound exposure amplitude and pulse modulation, contrast agent dose, and infusion rate. Results The quantitative method overcomes the limitation of visual scoring and works for a large dynamic range of treatment impact. Macrolesions are generated as an accumulation of probability driven microlesion formations. Macrolesions grow radially with radii from 0.1 to 1.6 mm as the ultrasound exposure amplitude (peak negative) increases from 2 to 4 MPa. To shorten treatment time, a swept beam was investigated and found to generate an acceptable macrolesion volume of about 40 μL for a single beam position. Conclusions Ultrasound parameters and administration of microbubbles directly influence lesion characteristics such as microlesion density and macrolesion dimension. For lesion generation planning, control of MCET is crucial, especially when targeting larger pre-clinical models.http://deepblue.lib.umich.edu/bitstream/2027.42/115462/1/40349_2015_Article_39.pd

The influence of octyl beta-D-glucopyranoside on cell lysis induced by ultrasonic cavitation

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/98658/1/JAS003482.pd